Cell proliferation, migration and CAM-dependent neurite outgrowth as developmental in vitro endpoints for screening teratogenic potential: Application to valproic acid and related analogues of varying potency

Toxicology in Vitro

Volumn 12, Issue 2, 1998, Pages 101-109

  Bacon, C L ^a   Berezin, V ^b   Bode, G ^c   Bock, E ^b   Bojic, U ^d   Doherty, P ^e   Ehlers, K ^d   Ellerbeck, U ^d   Julien, P ^c   Kawa, A ^b   Maar, T E ^b   Nau, H ^d   Pirovano, R ^f   Regan, C M ^a   Schousboe, A ^b   Spezia, F ^c   Walsh, F S ^e   Williams, E J ^e  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c SANOFI   (France)

^d UNIVERSITY OF VETERINARY MEDICINE HANNOVER   (Germany)

^e GUY S HOSPITAL   (United Kingdom)

^f Inst di Richerche Biomediche A M   (Italy)

Author keywords

Aggregation; C6 glioma; L1; Limb bud cells; N cadherin; Primary neurons

Indexed keywords

2 PROPYL 2 PENTENOIC ACID; 2 PROPYL 4 PENTYNOIC ACID; CALGRANULIN; CELL ADHESION MOLECULE; NERVE CELL ADHESION MOLECULE; UNCLASSIFIED DRUG; VALPROIC ACID;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CARTILAGE CELL; CELL AGGREGATION; CELL CYCLE G1 PHASE; CELL DIFFERENTIATION; CELL MIGRATION; CELL PROLIFERATION; CELL STRAIN; CONTROLLED STUDY; DOSE RESPONSE; DRUG SCREENING; EMBRYO; ENANTIOMER; FEMALE; GLIOMA CELL; IN VITRO STUDY; INTRAPERITONEAL DRUG ADMINISTRATION; LIMB BUD; MOUSE; NERVE FIBER GROWTH; NONHUMAN; RAT; TECHNIQUE; TERATOGENICITY;

EID: 0032055470     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0887-2333(97)00100-8     Document Type: Article

References (54)

1. Ahmed M., Barley G. C., Hearn M. T. W., Jones E. R. H., Thaller V. and Yates J. A. (1974) Natural acetylenes XLIII. Polyacetylenes from cultures of the fungus Fistulina pallida. Journal of the Chemical Society Perkin Transactions 1, 1981-1987.
2. Andrews J. E., Ebron-McCoy M., Bojic U., Nau H. and Kavlock R. J. (1995) Validation of an in vitro teratology system using chiral substances: Stereoselective teratogenicity of 4-yn-valproic acid in cultured mouse embryos. Toxicology and Applied Pharmacology 132, 310-316.
3. Berezin V., Kawa A., Bojic U., Foley A., Nau H., Regan C. and Bock E. (1996) Teratogenic potency of valproate analogues evaluated by quantitative estimation of cellular morphoiogy in vitro. Toxicology in Vitro 10, 585-594.
4. 2+-dependent and independent cell adhesion molecules. Proceedings of the National Academy of Sciences of the U.S.A. 84, 2555-2559.
5. Braun A. G., Buckner C. A., Emerson D. J. and Nichinson B. B. (1982) Quantitative correspondence between the in vivo and in vitro activity of teratogenic agents. Proceedings of the National Academy of Sciences of the U.S.A. 79, 20:56-2060.
6. Bronner-Fraser M., Wolf J. J. and Murray B. A. (1992) Effects of antibodies against N-cadherin and NCAM on the cranial neural crest and neural tube migration. Developments in Biology 153, 291-301.
7. Brown N. A. and Fabro S. (1983) The value of animal teratogenicity testing for predicting human risk. Clinical Obstetrics and Gynecology 26, 467-477.
8. Cerbai G., Turbanti L., Tellini N., Fabrizi P. and Dell'Omodarme G. (1871) Derivati acetilenici ad attivita antispastica Nota II - Aminoesteri dell'acido propilpropargilacetico. Farmaco Edizione Scientifica 27, 217-234.
9. Copp A. J., Brook F. A. and Roberts H. J. (1988) A cell-type-specific abnormality of cell proliferation in mutant (curly tail) mouse embryos developing spinal neural tube defects. Development 104, 285-295.
10. Courage-Maguire C, Bacon C. L., Regan C. M. and Nau H. (1996) Correlation of in vitro anti-proliferative potential with in vivo teratogenicity in a series of valproate analogues. International Journal of Developmental Neuroscience 15, 693-694.
11. Daston G. P. and d'Amato R. A. (1989) In vitro techniques in teratology. Toxicology and Industrial Health 5, 555-585.
12. de Moura Capos M. and do Amaral L. (1965) Neighboring group participation in addition reactions VI. Lactonisation of 4-pentenoic acid by iodine. Archives of Pharmacology 298, 92-100.
13. Doherty P., Ashton S., Moore S. E. and Walsh F. S. (1991) Morphoregulatory activities of NCAM and N-cadherin can be accounted for by G-protein dependent activation of L- and N-type neuronal calcium channels. Cell 67, 21-33.
14. Doherty P., Fruns M., Seaton P., Dickson G., Baron C. H., Sears T. A. and Walsh F. S. (1990) A threshold effect of the major isoforms of NCAM on neurite outgrowth. Nature 343, 464-466.
15. Doherty P., Skaper S., Moore S. E., Leon A. and Walsh F. S. (1992) A developmentally regulated switch in neuronal responsiveness to NCAM and N-cadherin in the rat hippocampus. Development 115, 885-892.
16. Doherty P. and Walsh F. S. (1994) Signal transduction events underlying neurite outgrowth stimulated by cell adhesion molecules. Current Opinions in Neurobiology 4, 49-55.
17. Ehlers K., Sturje H., Merker H. J. and Nau H. (1992) Valproic acid-induced spina bifida: a mouse model. Teratology 45, 145-154.
18. Elmazar M. M. A., Hauck R. S. and Nau H. (1993) Anticonvulsant and neurotoxic activities of twelve analogues of valproic acid. Journal of Pharmaceutical Science 82, 1255-1253.
19. Evans D. A. and Weber A. E. (1986) Asymmetric glycine enolate aldol reactions: Synthesis of cyclosporine's unusual amino acid, MeBmt. Journal of the American Chemical of Society 108, 6757-6761.
20. Fisher J. E., Jr, Acuff-Smith K. D., Schilling M. A., Nau H. and Vorhees C. V. (1994) Trans-2-ene valproic acid is less behaviourally teratogenic than an equivalent dose of valproic acid in rats. Teratology 49, 479-486.
21. Flint O. P. (1986) An in vitro test for teratogens: its practical application. Food and Chemical Toxicologv 24, 627-631.
22. Fox G. B., O'Connell A. W., Murphy K. J. and Regan C. M. (1995) Memory consolidation induces a transient and time-dependent increase in the frequency of NCAM polysialylated cells in the adult rat hippocampus. Journal of Neurochemistry 65, 2796-2799.
23. Geitz H., Handt S. and Zwingenberger R. (1996) Thalidomide selectively modulates the density of cell surface molecules involved in the adhesion cascade. Immunopharmacology 31, 213-221.
24. Hassell J. R. and Horigan E. A. (1982) Chondrogenesis: a model developmental system for measuring potential teratogenic compounds. Teratogenesis. Carcinogenesis and Mutagenesis 2, 325-331.
25. Hauck R. S., Elmazar M. M. A., Plum C. and Nau H. (1992) The enantioselective teratogenicity of 2-n-propyl-4-pentynoic acid (4-yn-VPA) is due to stereoselective intrinsic activity and not differences in pharmacokinetics. Toxicology Letters 60, 145-153.
26. Hauck R.-S. and Nau H. (1989) Asymmetric synthesis and enantioselective teratogenicity of 2-n-propyl-4-pentenoic acid (4-en-VPA), an active metabolite of the anticonvulsant drug, valproic acid. Toxicology Letters 49, 41-48.
27. Hauck R.-S. and Nau H. (1992) The enantiomers of the valproic acid analogue 2-n-propyl-4-pentynoic acid (4-yn-VPA): Asymmetric synthesis and highly stereoselective teratogenicity in mice. Pharmaceutical Research 9, 850-855.
28. Lammer E. J., Sever L. E, and Oakley G. P., Jr (1987) Teratogen update: valproic acid. Teratology 35, 465-473.
29. Landmesser L., Dahn L., Tang J. C. and Rutishauser U. (1990) Polysialic acid as a regulator of tramuscular nerve branching during embryonic development. Neuron 4, 655-667.
30. Maar T., Moran J., Schousboe A. and Rasantes-Morales H. (1995) Taurine deficiency in dissociated mouse cerebella cultures affects neuronal migration. International Journal of Developmental Neurosciences 13, 491-502.
31. Maguire C. and Regan C. M. (1991) In vitro screening for anticonvulsant-induced teratogenesis: drug alteration of cell adhesivity. International Journal of Developmental Neurosciences 9, 581-586.
32. Martin M. L., Breen K. C. and Regan C. M. (1988) Perturbations of cellular functions integral to neural tube formation by the putative teratogen sodium valproate. Toxicology in Vitro 2, 43-48.
33. Martin M. L. and Regan C. M. (1988) The anticonvulsant sodium valproate specifically induces the expression of a rat glial heat shock protein which is identified as the collagen type IV receptor. Brain Research 459, 131-137.
34. Martin M. L. and Regan C. M. (1991) The anticonvulsant valproate teratogen restricts the glial cell cycle at a defined point in the mid G1 phase. Brain Research 554, 223-228.
35. Moase C. E. and Trasler D. G. (1991) NCAM alterations in splotch neural tube defect mouse embryos. Development 113, 1049-1058.
36. Murphy K. J., O' Connell A. W. and Regan C. M. (1996) Repetitive and transient increases in hippocampal neural cell adhesion molecule polysialyiation state following multi-trial spatial training. Journal of Neurochemistry 67, 1268-1274.
37. Nau H. (1986) Species differencies in pharmacokinetics and drug teratogenesis. Environmental Health Perspectives 70, 113-129.
38. Nau H., Hauck R. S. and Ehlers K. (1991) Valproic acid-induced neural tube defects in mouse and human: aspects of chirality, alternative drug development, pharmacokinetics and possible nechanisms. Pharmacology and Toxicology 69, 310-321.
39. Nau U. and Loscher W. (1986) Pharmacologie evaluation of various metabolites and analogues of valproic acid: teratogenic potencies in mice. Fundamental and Applied Toxicology 6, 669-676.
40. Nau H. and Scott W. J. (1986) Weak acids may act as teratogens by accumulating in the basic milieu of the early mammalian embryo. Nature 323, 276-278.
41. Neubert D. and Chahoud I. (1985) Significance of species and strain differences in pre- and perinatal toxicology. Archives of Histochemistry 31 (Suppl.), 23-35.
42. Neubert R., Nogueira A. C and Neubert D. (1993) Thalidomid derivatives and the immune system. I Changes in the pattern of integrin receptors and other surface markers on T lymphocyte subpopulations of marmoset blood. Archives of Toxicology 67, 1-17.
43. Paulsen D. P. and Solursch M. (1988) Microtiter micromass cultures of limb-bud mesenchymal cells. In Vitro Cellular and Developmental Biology 24, 138-147.
44. Peters P. W. J. and Piersma A. H. (1990) In vitro embryotoxicity and teratogenicity studies. Toxicology in Vitro 4, 570-576.
45. Renault J. Y., Melcion C. and Cordier A. (1989) Limb bud cell cultures for in vitro teratogen screening: validation of an improved assessment method using 51 compounds. Teratogenesis. Carcinogenesis and Mutagenesis 9, 83-96.
46. Robert E. (1988) Valproic acid as a human teratogen. Congenital Anomalies 28 (Suppl.), S71-S80.
47. Sanford K. K., Earle W. R., Evens B. J., Waltz H. K. and Shannon J. E. (1951) The measurement of proliferation in tissue culture by enumeration of cell nuclei. Journal of the National Cancer Institute 11, 773-795.
48. Schardein J. L., Schweiz B. A. and Kenel M. F. (1985) Species sensitivities and prediction of teratogenic potential. Environmental Health Perspectives 61, 55-67.
49. Scott R. E., Florine D. L., Wille J. J., Jr and Yun K. (1982) Coupling of growth arrest and differentiation at a distinct state in the G1 phase of the cell cycle: GD. Proceedings of the National Academy of Sciences of the U.S.A. 79, 845-849.
50. Scott W. J., Wilson J. G., Riiter E. J. and Fradkin R. (1978) Further studies on distribution of teratogenic drugs in pregnant rats and rhesus monkeys. In Role of Pharmacokinetics in Prenatal and Perinatal Toxicology. Edited by D. Neubert, H. J. Merker. H. Nau and J. Langman, pp. 499-505. Georg Thieme. Stuttgart.
51. Voorhees C. V. (1987) Behavioral teratogenicity of valproic acid: Selective effects on behavior after prenatal exposure to rats. Psychopharmacology 92, 173-179.
52. Welsch F. (1992) In vitro approaches to the elucidation of mechanisms of chemical teratogenesis. Teratology 46, 3-14.
53. 2+ /calmodulin kinase inhibitor. KN-62, inhibits neurite outgrowth stimulated by CAMs and FGF. Molecular Cell Neuroscience, 69-79.
54. Williams E. J., Walsh F. S. and Doherty P. (1994) Tyrosine kinase inhibitors can differentially inhibit integrin and CAM stimulated neurite outgrowth. Journal of Cell Biology 124, 1029-1037.