In vivo electron paramagnetic resonance imaging of tumor heterogeneity and oxygenation in a murine model

Cancer Research

Volumn 58, Issue 7, 1998, Pages 1562-1568

  Kuppusamy, Periannan ^a   Afeworki, Mobae ^b   Shankar, Ravi A ^a   Coffin, Deborah ^b   Krishna, Murali C ^b   Hahn, Stephen M ^c   Mitchell, James B ^b   Zweier, Jay L ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

^c HOSPITAL OF THE UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; DIAGNOSTIC IMAGING; ELECTRON SPIN RESONANCE; FEMALE; MOUSE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE IMAGING; PRIORITY JOURNAL; THREE DIMENSIONAL IMAGING; TUMOR; TUMOR CELL;

ANIMALS; ELECTRON SPIN RESONANCE SPECTROSCOPY; FEMALE; IMAGE PROCESSING, COMPUTER-ASSISTED; KINETICS; MICE; MICE, INBRED C3H; NEOPLASMS, EXPERIMENTAL; NITROGEN OXIDES; OXIDATION-REDUCTION; OXYGEN;

EID: 0032054824     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (53)

1. Alexander, P., and Charlesby, A. Physico-chemical methods of protection against ionizing radiations. Radiobiol. Symp., 49: 1954.
2. Bacq, Z. M. Chemical protection against ionizing radiation, pp. 8-12. Springfield, IL: Charles C. Thomas, Inc., 1965.
3. Heppner, G. H. Tumor heterogeneity. Cancer Res., 44: 2259-2265, 1984.
4. Moulder, J. E., and Rockwell, S. Hypoxic fractions of solid tumors: experimental techniques, methods of analysis, and a survey of existing data. Int. J. Radiat. Oncol. Biol. Phys., 10: 695-712, 1984.
5. Brown, J. M, Hall, E. J., Hirst, D. G., Kinsella, T. J., Kligerman, M. M., Mitchell. J. B., Travis, E. J., and Valeriote, F. Chemical modification of radiation and chemotherapy. Am. J. Clin. Oncol., 11: 288-303, 1988.
6. Kennedy, K. A, Teicher, B. A., Rockwell, S., and and Sartorelli, A. C. The hypoxic tumor cell: a target for selective chemotherapy, Biochem. Pharmacol., 29: 1-8 1980.
7. Maltempo, M. M., Eaton, S. S., and Eaton, G. R. Spectral-spatial two-dimensional imaging. J. Magn. Reson., 72: 449-455, 1987.
8. Berliner, L. J., Fujii, H., Wan, X., and Lukiewicz, S. J. Feasibility study of imaging a murine tumor by electron paramagnetic resonance. Magn. Res. Med., 4: 380-384, 1987.
9. Woods, R. K., Dobrucki, J. W., Glockner, J. D., Morse, P. D., II, and Swartz, H. M. Spectral-spatial EPR imaging as a method for non-invasive biological oximetry. J. Magn. Reson., 85: 50-59, 1989.
10. Halpern, H. J., Spencer, D. P., Polen, J. V., Bowman, M. K., Nelson, A. C., Dowey, E. M., and Teicher, B. A. Imaging radio frequency electron spin resonance spectrometer with high resolution and sensitivity for in vivo measurements. Rev. Sci. Instrum., 60: 1040-1050, 1989.
11. Kuppusamy, P., Chzhan, M., Vij, K., Shteynbuk, M., Gianella, E., Lefer, D. J., and Zweier, J. L. Three dimensional spectral-spatial EPR imaging of free radicals in the heart: a technique for imaging tissue metabolism and oxygenation. Proc. Natl. Acad. Sci. USA, 91: 3388-3392, 1994.
12. Kuppusamy, P., Chzhan, M., and Zweier, J. L. Development and optimization of three-dimensional spatial EPR imaging for biological organs and tissues. J. Magn. Reson. B, 106: 122-130, 1995.
13. Kuppusamy, P., Chzhan, M., Samouilov, A., Wang, P., and Zweier, J. L. Mapping the spin density and lineshape distribution of free radicals using 4D spectral-spatial EPR imaging. J. Magn. Reson. B, 707: 116-125, 1995.
14. Halpern, H. J., Yu, C., Peric, M., Barth, E., Grdina, D. J., and Teicher, B. A. Oxymetry deep in tissues with low-frequency electron paramagnetic resonance. Proc. Natl. Acad. Sci. USA, 91: 13047-13051, 1994.
15. Kuppusamy, P., Shankar, R. A., and Zweier, J. L. In vivo measurement of arterial and venous oxygenation in the rat using 3D spectral-spatial electron paramagnetic resonance imaging. Phys. Med. Biol., in press, 1998.
16. Berliner, L. J. Spin Labeling: Theory and Applications, Vols. 1 and 2. New York: Academic Press, 1976.
17. Kocherginsky, N., and Swartz, H. M. Nitroxide Spin Labels. Reactions in Biology and Chemistry. Boca Raton, FL: CRC Press, 1995.
18. Couet, W. R., Brasch, R. C., Sosnovsky, G., and Tozer, T. N. Factors affecting nitroxide reduction in ascorbate solution and tissue homogenates. Magn. Reson. Imaging, 3: 83-88, 1985.
19. Swartz, H. M. Principles of the metabolism of nitroxides and their implication for spin trapping. Free Radical Res. Commun., 9: 399-405, 1990.
20. Ishida, S., Kumashiro, H., Tsushihashi, N., Ogata, T., Ono, M., Kamada, H., and Yoshida, E. In vivo analysis of nitroxide radicals injected into small animals by L-band esr technique. Phys. Med. Biol., 34: 1317-1323, 1989.
21. Berliner, L. J., and Wan, X. M. In vivo pharmacokinetics by electron magnetic resonance spectroscopy. Magn. Reson. Med., 9: 430-434, 1989.
22. Utsumi, H., Ichikawa, K., and Takeshita, K. In vivo ESR measurements of free radical reactions in living mice. Toxicol. Lett., 82/83: 561-565, 1995.
23. Kuppusamy, P., Wang, P., Zweier, J. L., Krishna, C. M., Mitchell, J. B., Ma, L., Trimble, C., and Hsia, C. J. EPR imaging of rat heart with nitroxide and a polynitroxylated albumin. Biochemistry, 35: 7051-7057, 1996.
24. Halpern, H. J., Peric, M., Cheng, Y., Barth, E. D., Chandramouli, G. V. R., Makinen, M., and Rosen, G. M. In vivo spin label murine pharmacodynamics using low-frequency electron paramagnetic resonance imaging. Biophys. J., 71: 403-409, 1996.
25. Chen, K., Glockner, J. F., Morse, P. D., II, and Swartz, H. M. Effects of oxygen on the metabolism of nitroxide spin labels in cells. Biochemistry, 28: 2496-2501, 1989.
26. Subczynski, W. K., Lukiewicz, S. J., and Hyde, J. S. Murine in vivo L-band spin-label oximetry with a loop-gap resonator. Magn. Reson. Med., 3: 747-754, 1986.
27. Zweier, J. L., and Kuppusamy, P. Electron paramagnetic resonance measurements of free radicals in the intact beating heart: a technique for detection and characterization of free radicals in whole biological tissues. Proc. Natl. Acad. Sci. USA 85: 5703-5707, 1988.
28. Bacic, G., Goda, F., Jiang, J., O'Hara, J. A, Dunn, J. F., and Swartz, H. M. Use of nitroxides for assessing perfusion, oxygenation, and viability of tissues: in vivo EPR and MRI studies. Magn. Reson. Med., 35: 97-106, 1996.
29. Mitchell, J. B., Samuni, A., Krishna, M. C., DeGraff, W. G., Ahn, M. S., Samuni, U., and Russo, A. Biologically active metal-independent Superoxide dismutase mimics. Biochemistry, 29: 2802-2807, 1990.
30. Samuni, A., Krishna, M. C., Mitchell, J. B., Collins C. R., and Russo, A. Superoxide reaction with nitroxides. Free Radical Res. Commun., 9: 241-249, 1990.
31. Samuni, A., Mitchell, J. B., DeGraff, W. G., Krishna, M. C., Samuni, U., and Russo, A. Nitroxide SOD-mimics. modes of action. Free Radical Res. Commun., 12-13: 187-194, 1991.
32. Samuni, A., Winkelsburg, D., Pinson, A., Hahn, S. M., Mitchell, J. B., and Russo, A. Nitroxide stable radicals protect beating cardiomyocytes against oxidative damage. J. Clin. Invest., 87: 1526-1530, 1991.
33. Krishna, M. C., Samuni, A., Taira, J., Goldstein, S., Mitchell, J. B., and Russo, A. Stimulation by nitroxides of catalase-like activity of hemeproteins. J. Biol. Chem., 271: 26018-26025, 1996.
34. Krishna, M. C., Russo, A., Mitchell, J. B., Goldstein, S., Dafni, H., and Samuni, A. Do nitroxide antioxidants act as scavengers of Superoxide or as SOD mimics? J. Biol. Chem., 271: 26026-26031, 1996.
35. Mitchell, J. B., DeGraff, W., Kaufman, D., Krishna, M. C., Samuni, A., Finkelstein, E., Ahn, M. S., Hahn, S. M., Gamson, J., and Russo, A. Inhibition of oxygen pendent radiation induced damage by the nitroxide Superoxide mimic. Arch. Biochem. Biophys., 289: 62-70, 1991.
36. Hahn, S. M., Wilson, L., Krishna, M. C., Liebmann, J., DeGraff, W. G., Gamson, J., Samuni, A., Venzon, D., and Mitchell, J. B. Identification of nitroxide radioprotectors. Radiat. Res., 132: 87-93, 1992.
37. Hahn, S. M., Tochner, Z., Krishna, M. C., Glass, J., Wilson, L., Samuni, A., Sprague, M., Venzon, D., Gladstein, E., Mitchell, J. B., and Russo, A. Tempol, a stable free radical is a novel murine radiation protector. Cancer Res., 52: 1750-1753, 1992.
38. Hahn, S. M., Sullivan, F. J., DeLuca, A. M., Krishna, C. M., Wersto, N., Venzon, D., Russo, A., and Mitchell, J. B. Evaluation of Tempol radioprotection in a murine tumor model. Free Radical Biol. Med., 22: 1211-1216, 1997.
39. Kuppusamy, P., Wang, P., and Zweier, J. L. Three-dimensional spatial EPR imaging of the rat heart. Magn. Reson. Med., 34: 99-105, 1995.
40. Walczak, T., and Swartz, H. M. A GHz in vivo ESR spectrometer with a surface probe. Physica Medica, 5: 195-202, 1989.
41. Kuppusamy, P., and Zweier, J. L. A forward subtraction procedure for removing hyperfine artifacts in electron paramagnetic resonance imaging. Magn. Reson. Med., 35: 316-322, 1996.
42. Marr, R. B., Chen, C. N., and Lauterbur, P. C. On two approaches to 3D reconstruction in NMR zeugmatography. in G. T. Herman and F. Natterer (eds.), Mathematical Aspects of Computerized Tomography, Vol. 8, pp. 225-240. New York: Springer-Verlag, 1981.
43. Woods, R. K., Hyslop, W. B., Maar, R. B., and Lauterbur, P. C. Image reconstruction. In: G. R. Eaton, S. S. Eaton, and K. Ohno (eds.), EPR Imaging and in Vivo EPR, pp. 91-117. Boca Raton, FL: CRC Press, 1991.
44. Belkin, S., Mehlhorn, R. J., Hideg, K., Hankovsky, O., and Packer, L. Reduction and destruction rates of nitroxide spin probes. Arch. Biochem. Biophys., 256: 232-243, 1987.
45. . Kuppusamy, P., Wang, P., and Zweier, J. L. Evaluation of nitroxides for the study of myocardial metabolism and oxygenation. Magn. Reson. Chem., 33: S123-S128, 1995.
46. 2 in response to perfluorocarbon/carbogen in FSa and NFSa murine fibrosarcoma with low frequency electron paramagnetic resonance oxymetry. Radiat. Res., 145: 610-618, 1996.
47. Krishna, M. C., Grahame, D. A., Samuni, A., Mitchell, J. B., and Russo, A. Oxoammonium cation intermediate in the nitroxides-catalyzed dismutation of superoxide. Proc. Natl. Acad. Sci. USA, 89: 5537-5541, 1992.
48. Miura, Y., Utsumi, H., and Hamada, A. Effects of inspired oxygen concentration on in vivo redox reaction of nitroxide radicals in whole mice. Biochem. Biophys. Res. Commun., 182: 1108-1114, 1992.
49. Quaresima, V., Ursini, C. L., Gualtieri, G., Sotgiu, A., and Ferrari, M. Oxygen-dependent reduction of a nitroxide free radical by electron paramagnetic resonance monitoring of circulating rat blood. Biochim. Biophys. Acta, 1182: 115-118, 1993.
50. Dobrucki, J. W., Demsar, F., Walczak, T., Woods, R. K., Bacic, G., and Swartz, H. M. Electron spin resonance microscopy of an in vitro tumor model. Br. J. Cancer 61: 221-224, 1990.
51. Dobrucki, J. W, Sutherland, R. M., and Swartz, H. M. Nonperturbing test for cytotoxicity in isolated cells and spheroids, using electron paramagnetic resonance. Magn. Reson. Med., 19: 42-55, 1991.
52. 2-histography. Int. J. Radiat. Oncol. Biol. Phys., 19: 953-961, 1990.
53. Kuppusamy, P., Wang, P., and Zweier, J. L. High-resolution electron paramagnetic resonance imaging of biological samples with a single line paramagnetic label. Magn. Reson. Med. 37: 479-483, 1997.