Expression in Escherichia coli of the putative N-acetylneuraminate lyase gene (nanA) from Haemophilus influenzae: Overproduction, purification, and crystallization

Protein Expression and Purification

Volumn 12, Issue 3, 1998, Pages 295-304

  Lilley, Glenn G ^a   Barbosa, João A R G ^a,b   Pearce, Lesley A ^a  

^a CSIRO   (Australia)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Crystallizati on; Escherichia coli; Expression; Gene; Haemophilus influenzae; Lyase; N acetylneuraminate; Overproduction; Purification

Indexed keywords

CRYSTALLIZATION; ENZYME ACTIVITY; ENZYME CONFORMATION; EXPRESSION VECTOR; N ACETYLNEURAMINATE LYASE; N ACETYLNEURAMINIC ACID ALDOLASE; PROTEIN PURIFICATION; SIALIC ACID ALDOLASE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HAEMOPHILUS INFLUENZAE; PROKARYOTA; TRIXIS;

EID: 0032053303     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1997.0841     Document Type: Article

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