Low energy loss electron microscopy of chromophores

Micron

Volumn 29, Issue 2-3, 1998, Pages 97-104

  Barfels, Melanie M G ^a   Jiang, Xungao ^a   Heng, Yew Meng ^b   Arsenault, A Larry ^b   Ottensmeyer, F Peter ^c  

^a UNIVERSITY OF TORONTO   (Canada)

^b MCMASTER UNIVERSITY   (Canada)

^c PRINCESS MARGARET HOSPITAL   (Canada)

Author keywords

Alcian blue; Cartilage; EELS; ESI; Hematin; Histochemistry; Proteoglycans; Resolution; Sensitivity

Indexed keywords

ALCIAN BLUE; COLORING AGENT; GLYCOSAMINOGLYCAN; HEMIN;

ANIMAL; ARTICLE; ELECTRON MICROSCOPY; GROWTH PLATE; INSTRUMENTATION; METHODOLOGY; RAT; SENSITIVITY AND SPECIFICITY; SPECTROPHOTOMETRY; ULTRASTRUCTURE;

ALCIAN BLUE; ANIMALS; COLORING AGENTS; GLYCOSAMINOGLYCANS; GROWTH PLATE; HEMIN; MICROSCOPY, ELECTRON; RATS; SENSITIVITY AND SPECIFICITY; SPECTROPHOTOMETRY;

ANIMALIA;

EID: 0032053267     PISSN: 09684328     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0968-4328(98)00009-2     Document Type: Article

References (46)

1. Adamson-Sharpe K. M., Ottensmeyer F. P., 1981. Spatial resolution and detection sensitivity in microanalysis by electron energy loss selected imaging. J. Microscopy, 122, 309-314.
2. Arsenault A. L., Ottensmeyer F. P., 1983. Quantitative spatial distribution of calcium, phosphorus and sulfur in calcifying epiphysis by high resolution spectroscopic imaging. Proc. Natl Acad. Sci. USA, 80, 1322-1326.
3. Atkins E. D. T., Phelps C. F., Sheehan K. J., 1972. The conformation of mucopolysaccharides. Biochem. J., 128, 1255-1263.
4. Batson P. E., 1986. High resolution energy loss spectrometer for the scanning transmission electron microscope. Rev. Sci. Instrum., 57, 43-48.
5. Batson P. E., 1988. Parallel detection for high-resolution electron energy loss studies in the scanning transmission electron microscope. Rev. Sci. Instrum., 59, 1132-1138.
6. Bauer R., 1988. Electron spectroscopic imaging: and advanced technique for imaging and analysis in transmission electron microscopy. Meth. Microbiol., 20, 113-145.
7. Bazett-Jones D. P., Ottensmeyer F. P., 1981. Phosphorus distribution in the nucleosome. Science, 211, 169-170.
8. Bazett-Jones D. P., Ottensmeyer F. P., 1982. DNA organization in nucleosomes. Can. J. Biochem., 60, 364-370.
9. Bendayan M. B., Barth R. F., Gingras D., Londoño I., Robinson P. T., Alam F., Adams D. M., Mattiazzi L., 1989. Electron spectroscopic imaging of high-resolution immunocytochemistry: use of boronated protein A. J. Histochem. Cytochem., 37, 573-580.
10. Castaing R., Henry L., 1962. Filtrage magnétique des vitesses en microscopie électronique. Compt. Rend. Acad. Sci. Paris, B, 255, 76-78.
11. Ciliax B. J., Kenneth L. K., Leapman R. D., 1993. Radiation damage of fluorinated organic compounds measured by parallel electron energy loss spectroscopy. Ultramicroscopy, 48, 13-25.
12. Crozier P. A., 1995. Quantitative elemental mapping of materials by energy-filtered imaging. Ultramicroscopy, 58, 157-174.
13. Egerton, R. F., Philips, J. G. and Whelan, M. J., 1974. Application of energy analysis in a transmission electron microscope. In Electron Microscopy, eds. J. V. Sanders and D. J. Goodchild, Vol. I, pp. 137-140. Australian Acad. Sci., Canberra.
14. Egerton, R. F. (1996) Electron Energy-Loss Spectroscopy in the Electron Microscope. New York, Plenum Press.
15. Fryer J. R., McConnell C. H., Zemlin F., Dorset D. L., 1992. Effect of temperature on radiation damage to aromatic organic molecules. Ultramicroscopy, 40, 163-169.
16. Glaeser, R. M., 1975. In Physical Aspects of Electron Microscopy and Microbeam Analysis, eds. B. M. Siegel and D. R. Beaman, p. 205. Wiley, New York.
17. Hainfeld J., Isaacson M., 1978. The use of electron energy loss spectroscopy for studying membrane architecture: a preliminary report. Ultramicroscopy, 3, 87-96.
18. Heinegård D., Oldberg Å., 1989. Structure and biology of cartilage and bone matrix noncollagenous macromolecules. FASEB J., 3, 2042-2051.
19. Henderson R., 1995. The potential and limitations of neutrons, electrons and x-rays for atomic resolution microscopy of unstained biological molecules. Q. Rev. Biophys., 28, 171-193.
20. Henkelman R. M., Ottensmeyer F. P., 1974. An energy filter for biological electron microscopy. J. Microsc., 102, 79-94.
21. Hunt J. A., Disko M. M., Behal S. K., Leapman R. D., 1995. Electron energy-loss chemical imaging of polymer phases. Ultramicroscopy, 58, 55-64.
22. Isaacson M., Johnson D., Crewe A. V., 1973. Electron beam excitation and damage of biological molecules: its implications for specimen damage in electron microscopy. Radiation Res., 55, 205-224.
23. Isaacson M., Langmore J. P., Rose H., 1974. Determination of the non-localization of the inelastic scattering of electrons by electron microscopy. Optik, 41, 92-96.
24. Isaacson, M., 1977. Specimen damage in the electron microscope. In Principles and Techniques of Electron Microscopy: Biological Applications, ed. M. A. Hayat, Vol. 7, pp. 1-78. VanNostrand Reinhold, New York.
25. Jiang X.-G., Ottensmeyer F. P., 1993. Optimization of a prism-mirror-prism imaging energy filter for high resolution electron microanalysis. Optik, 94, 88-95.
26. Kohl H., Rose H., 1985. Theory of image formation by inelastically scattered electrons in the electron microscope. Adv. Electronics Electron. Phys., 65, 173-227.
27. Korn A. P., Spitnik-Elson P., Elson D., Ottensmeyer F. P., 1983. Specific visualization of ribosomal RNA in the intact ribosome by electron spectroscopic imaging. Eur. J. Cell Biol., 31, 334-340.
28. Krivanek O. L., Gubbens A. L., Dellby N., Meyer C. E., 1992. Design and first application of a post-column imaging filter. Microsc. Microanal. Microstruct., 2, 187-199.
29. Lanio S., Rose H., Krahl D., 1986. Test and improved design of a corrected imaging magnetic energy filter. Optik, 73, 56-68.
30. Lindahl U., Höök M., 1978. Glycosaminoglycans and their binding to biological macromolecules. Ann. Rev. Biochem., 47, 385-417.
31. Maitland M. E., Arsenault A. L., 1989. Freeze-substitution staining of rat growth plate cartilage with Alcian blue for electron microscopic study of proteoglycans. J. Histochem. Cytochem., 37, 383-387.
32. Muller D. A., Silcox J., 1995. Delocalization in inelastic scattering. Ultramicroscopy, 59, 195-213.
33. Ottensmeyer F. P., Andrew J. W., 1980. High resolution microanalysis of biological specimens by electron energy loss spectroscopy and electron spectroscopic imaging. J. Ultrastruct. Res., 72, 336-348.
34. Ottensmeyer F. P., 1982. Scattered electrons in microscopy and microanalysis. Science, 215, 461-466.
35. Ottensmeyer, F. P., 1984. Electron spectroscopic imaging: parallel energy filtering and microanalysis in the fixed beam electron microscope. J. Ultrastruct. Res., 88, 121-134.
36. Ottensmeyer F. P., 1986. Elemental mapping by electron energy filtration: advantages, limitations and compromises. Ann. New York Acad. Sci., 483, 339-353.
37. Reimer, L., 1995. Energy-filtering Transmission Electron Microscopy. Springer, Berlin.
38. Rose H., 1987. The retarding Wien filter as a high-performance imaging filter. Optik, 77, 26-34.
39. Rosenberg L., Hellmann W., Kleinschmidt A. K., 1975. Electron microscopic studies of proteoglycan aggregates from bovine articular cartilage. J. Biol. Chem., 250, 1877-1983.
40. Schattschneider, P. 1986. Fundamentals of Inelastic Electron Scattering. Springer, Vienna.
41. Simon, G. T. and Heng, Y. M., 1986. Standards for quantification of elemental analysis by energy dispersive spectroscopy (EDS) and electron spectroscopic imaging (ESI). In Proc. 11th Intl. Congr. X-ray Optics and Microanal., eds. Brown, J. D. and Packwood, R. H., Graphic Services, University of Western Ontario, London, Canada, pp. 416-419.
42. Spurr A. R., 1969. A low viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res., 26, 31.
43. Sun S. Q., Shi S.-L., Hunt J. A., Leapman R. D., 1994. Quantitative water mapping of cryosectioned cells by electron energy-loss spectroscopy. J. Microsc., 177, 18-30.
44. Uhlemann S., Rose H., 1994. The mandoline filter - a new high-performance imaging filter for sub-eV EFTEM. Optik, 96, 163-178.
45. Varlot K., Martin J. M., Quet C., Kihn Y., 1997. Towards sub-nanometer scale EELS analysis of polymers in the TEM. Ultramicroscopy, 68, 123-133.
46. Wang Z. L., 1996. Valence electron excitations and plasmon oscillations in thin firms surfaces interfaces and small particles. Micron, 27, 265-299.