Recombinant fusion proteins for the industrial production of disulfide bridge containing peptides: Purification, oxidation without concatamer formation, and selective cleavage

Protein Expression and Purification

Volumn 12, Issue 3, 1998, Pages 404-414

  Döbeli, Heinz ^a   Andres, Herbert ^a   Breyer, Nicola ^a   Draeger, Nicholas ^a   Sizmann, Dorothea ^a   Zuber, Maria Tomás ^a   Weinert, Brian ^a   Wipf, Beat ^a  

^a Preclinical Research   (Switzerland)

Author keywords

Human immunodeficiency virus (HIV); Selective cleavage of recombinant fusion proteins; Suppression of concatamer formation

Indexed keywords

PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; RECOMBINANT FUSION PROTEIN;

ESCHERICHIA COLI; HUMAN IMMUNODEFICIENCY VIRUS;

EID: 0032053024     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1997.0844     Document Type: Article

References (12)

1. Kudryavtseva E. V., Sidorova M. V., Ovchinnikov M. V., Bespalova Z. D., Bushuev V. N. Comparative evaluation of different methods for disulfide bond formation in synthesis of the HIV-2 antigenic determinant. J. Pept. Res. 49:1997;52-58.
2. Poumbourios P., McPhee D. A., Kemp B. E. Antibody epitopes sensitive to the state of human immunodeficiency virus type 1 gp41 oligomerization map to a putative alpha-helical region. AIDS Res. Hum. Retroviruses. 8:1992;2055-2062.
3. Chan D. C., Fass D., Berger J. M., Kim P. S. Core structure of gp41 from the HIV envelope glycoprotein. Cell. 89:1997;263-273.
4. De Jong J., Simon F., Van der Groen G., Baan E., Saragosti S., Brun-Vezinet F., Goudsmit J. V3 loop sequence analysis of seven HIV type 1 group O isolates phenotyped in peripheral blood mononuclear cells and MT-2 cells. AIDS Res. Hum. Retroviruses. 12:1996;1503-1507.
5. Hochuli E., Bannwarth W., Döbeli H., Gentz R., Stüber D. Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent. Bio/Technology. 6:1988;1321-1325.
6. Stüber D., Matile H., Garotta G. System for high-level production inEscherichia coli. Immunol. Methods. 4:1990;121-152.
7. Döbeli H., Draeger N., Huber G., Jakob P., Schmidt D., Seilheimer B., Stüber D., Wipf B., Zulauf M. A biotechnological method provides access to aggregation competent monomeric Alzheimer's 1-42 residue amyloid peptide. Bio/Technology. 13:1995;988-993.
8. Andres, H. Baumann, A. Baumann, E. A. Bürgisser, P. Döbeli, H. Erb, P. Frei, P. Gassner, D. Schönbrunner, R. Sizmann, D. Stieger, M. Verdon, B. Wanger, M. Wernli, M. 1994, Performance of a New, Fully Automated Double Antigen Sandwich (DAGS) Assay for the Detection of HIV-1/HIV-2 Antibodies, 1994.
9. Kane J. F. Effects of rare codons on high-level expression of heterologous proteins inEscherichia coli. Curr. Opin. Biotechnol. 6:1995;494-500.
10. Petit M. A., Maillard P., Capel F., Pillot J. Immunochemical structure of the hepatitis B surface antigen vaccine. II. An analysis of antibody response in human sera against the envelope proteins. Mol. Immunol. 23:1986;511-523.
11. Hochuli E., Döbeli H., Schacher A. New metal chelate adsorbent selective for proteins and peptides containing neighboring histidine residues. J. Chromatogr. 411:1987;177-184.
12. Nakane P. K., Kawaoi A. Peroxidase-labeled antibody: A new method of conjugation. J. Histochem. Cytochem. 22:1974;1084-1091.