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3843101168
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note
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Synthesis of amino acid-derivatized diacetylene lipids was based on a method described by W. Spevak. (Doctoral Thesis, University of California at Berkeley, 1993). In short, 10,12-pentacosadiynoic acid was converted to a succinimidyl ester by EDC and NHS, followed by reaction with amino acids in slightly basic THF/water solution. The product was extracted, filtered, dried, and allowed to pass through a silica gel column. Appropriate fractions were collected, and the sample was characterized by mass spectrometry. The liposomes were formed through sonication of lipids in pure water.
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8
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3843076008
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note
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Colorimetric response (CR) is defined in ref 2. In this study, CR is obtained as the percent change in the adsorption at 632 nm (blue color) with respect to the total absorption at 544 nm (red color) and 632 nm.
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