Dihydroorotate (dho(ut) and orotate (oro(ut)) utilizer mutants in yeast: Identification of the dho(ut) mutation and allelism of the DHO and URE2 genes;MUTANTS UTILISATEURS DE DIHYDROOROTATE ET D'OROTATE CHEZ LA LEVURE. CLONAGE DU GENE CORRESPONDANT A DHO(UT) ET RELATIONS D ALLELISME AVEC URE2

Comptes Rendus de l'Academie des Sciences - Serie III

Volumn 321, Issue 4, 1998, Pages 267-274

  Bloch, Jean Claude ^a   Pfeiffer, Pierre ^b   Exinger, Françoise ^c  

^a Inst de Biol Molec des P ^*  (France)

^b INST DE BIOL MOLEC DES PLANTES   (France)

^c IRCAD   (France)

Author keywords

5 fluoroorotate; Nitrogen regulation; Pyrimidine biosynthesis; Saccharomyces cerevisiae; URE2

Indexed keywords

FLUOROOROTIC ACID; OROTIC ACID; OROTIC ACID DERIVATIVE; 4,5 DIHYDROOROTIC ACID; 4,5-DIHYDROOROTIC ACID; DRUG DERIVATIVE; FUNGAL PROTEIN; PYRIMIDINE DERIVATIVE; SACCHAROMYCES CEREVISIAE PROTEIN; URE2 PROTEIN, S CEREVISIAE;

ALLELE; ARTICLE; GENE MUTATION; GENETIC COMPLEMENTATION; NONHUMAN; PYRIMIDINE SYNTHESIS; RADIATION MUTAGENESIS; SACCHAROMYCES CEREVISIAE; ULTRAVIOLET RADIATION; YEAST; BIOSYNTHESIS; CHROMOSOME MAP; GENETICS; METABOLISM; MOLECULAR CLONING; MUTAGENESIS; PRION;

SACCHAROMYCES CEREVISIAE;

ALLELES; CHROMOSOME MAPPING; CLONING, MOLECULAR; FUNGAL PROTEINS; MUTAGENESIS; OROTIC ACID; PRIONS; PYRIMIDINES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; ULTRAVIOLET RAYS;

EID: 0032034250     PISSN: 07644469     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0764-4469(98)80050-4     Document Type: Article

References (33)

1. [1] Lacroute F., Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae, J. Bacteriol. 95 (1968) 824-832.
2. [2] Lacroute F., The use of mutants in metabolic studies, in: Methods in Cell Biology, Vol. XI, Academic Press, New York, 1975, pp. 235-245.
3. [3] Losson R., Fuchs R.P.P., Lacroute F., Yeast promoters URA1 and URA3: Examples of positive control, J. Mol. Biol. 185 (1985) 65-81.
4. [4] Potier S., Lacroute F., Hubert J.C., Souciet J.L., Studies on transcription of the yeast URA2 gene, FEMS Microbiol. Lett. 72 (1990) 215-220.
5. [5] Drillien R., Lacroute F., Ureidosuccinic acid uptake in yeast and some aspects of its regulation, J. Bacteriol. 109 (1972) 203-208.
6. [6] Grenson M., Dubois E., Piotrowka M., Drillien R., Aigle M., Ammonia assimilation in Saccharomyces cerevisiae as mediated by the two glutamate dehydrogenases: evidence for the gdhA locus being a structural gene for the NADP-dependent glutamate dehydrogenase, Molec. Gen. Genet. 128 (1974) 73-85.
7. e S. T III (1962).
8. [8] Boeke J.D., Lacroute F., Fink G.R., A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoroorotic acid resistance, Mol. Gen. Genet. 197 (1984) 345-346
9. [9] Coshigano P.W., Magasanik B., The URE2 gene product of Saccharomyces cerevisiae plays an important role in the cellular response to the nitrogen source and has homology to glutathione S-transferases, Mol. Cell. Biol. 11 (1991) 822-832.
10. [10] Wickner R.B., [URE3] as an altered URE2 protein: Evidence for a prion analog in Saccharomyces cerevisiae, Science 264 (1994) 566-569.
11. [11] Masison D.C., Wickner R.B., Prion-inducing domain of yeast Ure2p and protease resistance of Ure2p in prion-containing cells, Science 270 (1995) 93-95.
12. [12] Mortimer R.K., Hawthorne D.C., Genetic mapping in Saccharomyces, Genetics 53 (1966) 165-173.
13. [13] Olempska-Beer Z., Bautz-Freese E.B., Optimal extraction conditions for high-performance liquid chromatographic determination of nucleotides in yeast, Anal. Biochem. 140 (1984) 236-245.
14. [14] Prescott L.M., Jones M.E., Modified methods for the determination of carbamyl phosphate, Anal. Biochem. 32 (1969) 408-419.
15. [15] Wolcott J.H., Ross C., Orotidine-5′-phosphate decarboxylase from higher plants, Biochim. Biophys. Acta 122 (1966) 532-534.
16. [16] Hupe D.J., Behrens N.D., A method for assaying orotate phosphoribosyltransferase and measuring phosphoribosyl pyrophosphate, Anal. Biochem. 161 (1987) 20-25.
17. [17] Clewell D.B., Nature of colE-1 plasmid replication in Escherichia coli in the presence of chloramphenicol, J. Bacteriol. 110 (1972) 667-676.
18. [18] Holmes S.D., Quigley M., A rapid boiling method for the preparation of bacterial plasmids, Anal. Biochem. 114 (1981) 193-197.
19. [19] Struhl K., Stinchcomb D.T., Scherer S., Davis R.W., High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules, Proc. Nat. Acad. Sci. USA. 76 (1979) 1035-1039.
20. [20] Hoffman C.S., Winston F., A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli, Gene 57 (1987) 267-272.
21. [21] Hinnen A., Hicks J.B., Fink G.R., Transformation of yeast, Proc. Natl. Acad. Sci. USA 75 (1978) 1929-1933.
22. [22] Chevallier M.R., Bloch J.C., Lacroute F., Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeast, Gene 11 (1980) 11-19.
23. [23] Lai E., Birren B.W., Clark S.M., Simon M.I., Hood L., Pulsed field gel electrophoresis, Biotechniques 7 (1989) 34-42.
24. [24] Gustavson G.D., Milner J.J., McFarland J.E., Pedersen K., Larkins B.A., Jackson A.O., Investigation of the complexity of barley stripe mosaic virus RNAs with recombinant DNA clones, Virology 120 (1982) 182-193.
25. [25] Bonneaud N., Ozier-Kalogeropoulos O., Li G., Labouesse M., Minvielle-Sebastia L., Lacroute F., A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors, Yeast 7 (1991) 609-615.
26. [26] Vernet T., Dignard D., Thomas D.Y., A family of yeast expression vectors containing the phage f1 intergenic region, Gene 52 (1991) 225-233.
27. [27] Orr-Weaver T.L., Szostak J.W., Rothstein R.J., Genetic applications of yeast transformation with linear and gapped plasmid, in: Part C.R., Wu L., Grossman, Moldave K. (Eds.), Methods in Enzymology, Vol. 101, Recombinant DNA, Academic Press, New York, 1983, pp. 228-245.
28. [28] Fuchs M., Pinck M., Serghini M.A., Ravelanandro M., Walter B., Pinck L., The nucleotide sequence of satellite RNA in grapevine fanleaf virus, strain F13, J. Gen. Virol. 70 (1989) 955-962.
29. [29] Hinnebusch A.G., Mechanisms of gene regulation in the general control of aminoacid biosynthesis in Saccharomyces cerevisiae, Microbiol. Rev. 52 (1988) 248-273.
30. [30] Cooper T.G., Nitrogen metabolism in Saccharomyces cerevisiae, in: Strathern J.N. et al. (Eds.), The Biology of the Yeast Saccharomyces. Metabolism and Gene Expression, Cold Spring Harbor Laboratory, New York, 1982, p. 39.
31. [31] Turoscy V., Cooper T.G., Ureidosuccinate is transported by the allantoate transport system in Saccharomyces cerevisiae, J. Bacteriol. 169 (1987) 2598-2600
32. [32] Cooper T.G., Ferguson D., Rai R., Bysani N., The GLN3 gene product is required for transcriptional activation of allantoin system gene expression in Saccharomyces cerevisiae, J. Bacteriol. 172 (1990) 1014-1018.
33. [33] Magasanik B., in: Jones E.W., Pringle J.R. (Eds.), The Molecular and Cellular Biology of the Yeast Saccharomyces cerevisiae, Cold Spring Harbor Laboratory Press, Plainview, NY, 1992, pp. 283-317.