Localization of 5-HT2A receptor in rat cerebral cortex and olfactory system revealed by immunohistochemistry using two antibodies raised in rabbit and chicken

Molecular Brain Research

Volumn 54, Issue 2, 1998, Pages 199-211

  Hamada, Shun ^a   Senzaki, Kouji ^a   Hamaguchi Hamada, Kayoko ^a   Tabuchi, Katsuhiko ^a   Yamamoto, Hideko ^b   Yamamoto, Toshifumi ^c   Yoshikawa, Shingo ^a   Okano, Hideyuki ^a,d,e   Okado, Nobuo ^a  

^a UNIVERSITY OF TSUKUBA   (Japan)

^b TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE   (Japan)

^c YOKOHAMA CITY UNIVERSITY   (Japan)

^d OSAKA UNIVERSITY MEDICAL SCHOOL   (Japan)

^e JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

Antibody; Immunocytochemistry; Rat; Receptor; Serotonin

Indexed keywords

GLIAL FIBRILLARY ACIDIC PROTEIN; GLUTATHIONE TRANSFERASE; POLYCLONAL ANTIBODY; SEROTONIN 2A RECEPTOR;

AMINO TERMINAL SEQUENCE; ANIMAL TISSUE; ARTICLE; BRAIN CORTEX; CARBOXY TERMINAL SEQUENCE; CHICKEN; CLAUSTRUM; FEMALE; IMMUNOELECTRON MICROSCOPY; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; MALE; MAMMILLARY BODY; MOTONEURON NUCLEUS; NEOCORTEX; NEUROPIL; NONHUMAN; OLFACTORY BULB; OLFACTORY SYSTEM; PRIORITY JOURNAL; PYRIFORM CORTEX; RABBIT; RAT; RED NUCLEUS;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES; AUTORADIOGRAPHY; CELL LINE; CEREBRAL CORTEX; CHICKENS; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; MALE; MICE; MOLECULAR SEQUENCE DATA; OLFACTORY PATHWAYS; RABBITS; RATS; RATS, WISTAR; RECEPTOR, SEROTONIN, 5-HT2A; RECEPTORS, SEROTONIN; SUBCELLULAR FRACTIONS; TRANSFECTION;

ANIMALIA; GALLUS GALLUS; ORYCTOLAGUS CUNICULUS;

EID: 0032032619     PISSN: 0169328X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0169-328X(97)00322-7     Document Type: Article

References (42)

1. Adams J.C. Heavy metal intensification of DAB-based HRP reaction product. J. Histochem. Cytochem. 29:1981;775.
2. Altar C.A., Kim H., Marshall J.F. Computer imaging and analysis of dopamine (D2) and serotonin (S2) binding sites in rat basal ganglia or neocortex labeled by [3H]spiroperidol. J. Pharmacol. Exp. Ther. 233:1985;527-538.
3. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. Basic local alignment search tool. J. Mol. Biol. 215:1990;403-410.
4. Arranz M., Collier D., Sodhi M., Ball D., Roberts G., Price J., Sham P., Kerwin R. Association between clozapine response and allelic variation in 5-HT2A receptor gene. Lancet. 346:1995;281-282.
5. Blin J., Baron J.C., Dubois B., Crouzel C., Fiorelli M., Attar-Levy D., Pillon B., Fournier D., Vidallhet M., Agid Y. Loss of brain 5-HT2 receptors in Alzheimer's disease. Brain. 116:1993;497-510.
6. Blue M.E., Yagaloff K.A., Mamounas L.A., Hartig P.R., Molliver M.E. Correspondence between 5-HT2 receptors and serotonergic axons in rat neocortex. Brain Res. 453:1988;315-328.
7. Carlin R.K., Grab D.J., Cohen R.S., Siekevitz P. Isolation and characterization of postsynaptic densities from various brain regions: Enrichment of different types of postsynaptic densities. J. Cell Biol. 86:1980;831-843.
8. Cross A.J. Serotonin in Alzheimer-type dementia and other dementing illnesses. Ann. N.Y. Acad. Sci. 600:1990;405-415.
9. Cross A.J., Crow T.J., Johnson J.A., Perry E.K., Perry R.H., Blessed G., Tomlinson B.E. Studies on neurotransmitter receptor systems in neocortex and hippocampus in senile dementia of the Alzheimer-type. J. Neurol. Sci. 64:1984;109-117.
10. Deecher D.C., Wilcox B.D., Dave V., Rossman P.A., Kimelberg H.K. Detection of 5-hydroxytryptamine-2 receptors by radioligand binding, northern blot analysis, and Ca2+ responses in rat primary astrocyte cultures. J. Neurosci. Res. 35:1993;246-256.
11. Dournaud P., Gu Y.Z., Schonbrunn A., Mazella J., Tannenbaum G.S., Beaudet A. Localization of the somatostatin receptor SST2A in rat brain using a specific anti-peptide antibody. J. Neurosci. 16(14):1996;4468-4478.
12. Duinkerke S.J., Botter P.A., Jansen A.A.I., Van Dongen P.A.M., Van Haaften A.J., Boom A.J., Van Laarhoven J.H.M., Busard H.L.S.M. Ritanserin, a selective 5-HT2/1C antagonist, and negative symptoms in schizophrenia: a placebo-controlled double-blind trial. Br. J. Psychiat. 163:1993;451-455.
13. Garlow S.J., Morilak D.A., Dean R.R., Roth B.L., Ciaranello R.D. Production and characterization of a specific 5-HT2 receptor antibody. Brain Res. 615:1993;113-120.
14. F.G. Graeff, The periaqueductal gray, 5-HT and anxiety, in: P.B. Bradley, S.L. Handley, S.J. Cooper, B.J. Key, N.M. Barnes, J.H. Coote (Eds.), Serotonin, CNS Receptors and Brain Function, Advances in the Biosciences, vol. 85, Pergamon, Oxford, 1992, pp. 219-233.
15. Hunt C.A., Schenker L.J., Kennedy M.B. PSD-95 is associated with the postsynaptic density and not with the presynaptic membrane at forebrain synapses. J. Neurosci. 16(4):1996;1380-1388.
16. Iyo M., Yamasaki T. The detection of age-related decrease of dopamine D1, D2 and serotonin 5-HT2 receptors in living human brain. Prog. Neuro-Psychopharmpharmacol. Biol. Psychiat. 17:1993;415-421.
17. Johnson D.G., De C Nogueria Araujo G.M. A simple method of reducing the fading of immunofluorescence during microscopy. J. Immunol. Methods. 43:1981;349-350.
18. Julius D., Huang K.N., Livelli T.J., Axel R., Jessell T.M. The 5-HT2 receptor defines a family of structurally distinct but functionally conserved serotonin receptors. Proc. Natl. Acad. Sci. USA. 87:1990;928-932.
19. Kornau H.C., Schenker L.T., Kennedy M.B., Seeburg P.H. Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95. Science. 269:1995;1737-1740.
20. Mann J.J., Arango V., Underwood M.D. Serotonin and suicidal behaviour. Ann. N.Y. Acad. Sci. 600:1990;476-484.
21. Marek G.J., Aghajanian G.K. Serotonergic amplification of apically-derived EPSPs in neocortical pyramidal cells via a persistent sodium current. Soc. Neurosci. Abstr. 22(2):1996;525.9.
22. Martin G.R., Humphrey P.P.A. Classification Review. Receptors for 5-hydroxytyptamine: current perspectives on classification and nomenclature. Neuropharmacology. 33(3/4):1994;261-273.
23. McLean J.H., Darby-King A., Paterno G.D. Localization of 5-HT2A receptor mRNA by in situ hybridization in the olfactory bulb of the postnatal rat. J. Comp. Neurol. 353:1995;371-378.
24. Mengod G., Pompeiano M., Martinez-Mir M.I., Palacios J.M. Localization of the mRNA for the 5-HT2 receptor by in situ hybridization histochemistry. Correlation with the distribution of receptor sites. Brain Res. 524:1990;139-143.
25. Morilak D.A., Garlow S.J., Ciaranello R.D. Immunocytochemical localization and description of neurons expressing serotonin-2 receptors in the rat brain. Neuroscience. 54(3):1993;701-717.
26. Nabeshima T., Kawashima K., Kameyama T. Effect of minaprine on cycloheximide-induced amnesia in mice. Eur. J. Pharmacol. 169:1989;249-257.
27. Nakada M.T., Wieczorek C.M., Rainbow T.C. Localization and characterization by quantitative autoradiography of [125I]LSD binding sites in rat brain. Neurosci. Lett. 49:1984;13-18.
28. Niitsu Y., Hamada S., Hamaguchi K., Mikuni M., Okado N. Regulation of synapse density by 5-HT2A receptor agonist and antagonist in the spinal cord of chicken embryo. Neurosci. Lett. 195:1995;159-162.
29. Ntakarutimana V., Demedts P., van Sande M., Scharpe S. A simple and economical strategy for downstream processing of specific antibodies to human transferrin from egg yolk. J. Immunol. Methods. 153:1992;133-140.
30. Ohno M., Yamamoto T., Watanabe S. Blockade of 5-HT2 receptors protects against impairment of working memory following transient forebrain ischemia in the rat. Neurosci. Lett. 129:1991;185-188.
31. Pazos A., Cortes R., Palacios J.M. Quantitative autoradiographic mapping of serotonin receptors in the rat brain. II. Serotonin-2 receptors. Brain Res. 346:1985;231-249.
32. Pompeiano M., Palacios J.M., Mengod G. Distribution of the serotonin 5-HT2 receptor family mRNAs: comparison between 5-HT2A and 5-HT2C receptors. Mol. Brain Res. 23:1994;163-178.
33. Saiki R.K., Gelfand D.H., Stoffel S., Scharf S.J., Higuchi R., Horn G.T., Mullis K.B., Erlich H.A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 239:1988;487-491.
34. Schneider I. Cell lines derived from late embryonic stages of Drosophila melanogaster. J. Embryol. Exp. Morph. 27(2):1972;353-365.
35. Smith D.B., Johnson K.S. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. 67:1988;31-40.
36. Tam J.P. Synthetic peptide vaccine design: synthesis and properties of a high-density multiple antigenic peptide system. Proc. Natl. Acad. Sci. USA. 85:1988;5409-5413.
37. Thummel C.S., Pirrotta V. New CaSpeR P-element vectors. Drosophila Info. Newslett. 2:1991.
38. 125I] AZIK). FEBS Lett. 213(2):1987;359-364.
39. Wright D.E., Seroogy K.B., Lundgren K.H., Davis B.M., Jennes L. Comparative localization of serotonin 1A, 1C and 2 receptor subtype mRNAs in rat brain. J. Comp. Neurol. 351:1995;357-373.
40. Yang W., Chen K., Lan N.C., Gallaher T.K., Shih J.C. Gene structure and expression of the mouse 5-HT2 receptor. J. Neurosci. Res. 33:1992;196-204.
41. Yates M., Leake A., Candy J.M., Fairbairn A.F., McKeith I.G., Ferrier I.N. 5-HT2 receptor changes in major depression. Biol. Psychiat. 27:1990;489-496.
42. Zamboni L., DeMartino C. Buffered picric acid-formaldehyde: a new rapid fixation for electron microscopy. J. Cell. Biol. 35:1967;148A.