Fluorescence microscopy of etched methacrylate sections improves the study of mitosis in plant cells

Microscopy Research and Technique

Volumn 40, Issue 5, 1998, Pages 369-376

  Hoffman, John C ^a   Vaughn, Kevin C ^a   Mullins, J Michael ^b  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b CATHOLIC UNIVERSITY OF AMERICA   (United States)

Author keywords

Anti mitotic antibody; Microtubule; Oat root; Serial sections; Spermatogenous cell

Indexed keywords

ANTIBODIES; CYTOLOGY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; TISSUE;

ANTI-MITOTIC ANTIBODY; CYTOSKELETONS; FOCAL PLANE; INTRACELLULAR STRUCTURES; MICROTUBULES; OAT ROOT; PLANT TISSUES; PLANTS CELLS; SERIAL SECTION; SPERMATOGENOUS CELL;

CELLS;

ARTICLE; CYTOSKELETON; FLUORESCENCE MICROSCOPY; MITOSIS; NONHUMAN; OAT; PLANT CELL; PLANT ROOT; PRIORITY JOURNAL;

STAPHYLOCOCCUS PHAGE 187;

EID: 0032031976     PISSN: 1059910X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0029(19980301)40:5<369::AID-JEMT4>3.0.CO;2-M     Document Type: Article

References (25)

1. Baskin, T.I., Busby, C.H., Fowke, L.C., Sammut, M., and Gubler, F. (1992) Improvements in immunostaining samples embedded in methacrylate: Localization of microtubules and other antigens throughout developing organs in plants of diverse taxa. Planta, 187:405-413.
2. Baskin, T.I., Wilson, J.E., Cork, A., and Williamson, R.E. (1994) Morphology and microtubule organization in Arabidopsis roots exposed to oryzalin and taxol. Plant Cell Physiol., 35:935-942.
3. Brown, R.C., Lemmon, B.E., and Mullinax, J.B. (1989) Immunofluorescent staining of microtubules in plant tissue: Improved embedding and sectioning techniques using polethylene glycol (PEG) and Steedman's wax. Bot. Acta., 102:54-61.
4. Davis, F.M., Tsao, T., Fowler, S., and Rao, P. (1983) Monoclonal antibodies to mitotic cells. Proc. Natl. Acad. Sci. U.S.A., 80:2926-2930.
5. Franke, W.W., Seib, E., Osborn, M., Weber, K., Herth, W., and Falk, H. (1977) Tubulin-containing structures in the anastral mitotic apparatus of endosperm cells of the plant Leucojum aestivum as revealed by immunofluorescence microscopy. Cytobiologie, 15:24-48.
6. Gubler, F. (1989) Immunofluorescence localization of microtubules in plant root tips embedded in butyl-methyl methacrylate. Cell Biol. Int. Rep., 13:137-145.
7. Hoffman, J.C., and Mullins, J.M. (1990) Nuclear and mitotically enhanced epitope. Cell Motil. Cytoskeleton, 16:68-79.
8. Hoffman, J.C., and Vaughn, K.C. (1994a) Griseofulvin destabilizes plant microtubules. Cytobios, 77:253-258.
9. Hoffman, J.C., and Vaughn, K.C. (1994b) Mitotic disrupter herbicides act by a single mechanism but vary in efficacy. Protoplasma, 179:16-25.
10. Hoffman, J.C., and Vaughn, K.C. (1995a) Using the developing spermatogenous cells of Ceratopteris to unlock the mysteries of the plant cytoskeleton. Int. J. Plant Sci. 156:346-358.
11. Hoffman, J.C., and Vaughn, K.C. (1995b) Post-translational tubulin modifications in spermatogenous cells of the pteridophyte Ceratopteris richardii. Protoplasma, 186:169-182.
12. Hoffman, J.C., Vaughn, K.C., and Joshi, H.C. (1994) Structural and immunocytochemical characterization of microtubule organizing centers in pteridophyte spermatogenous cells. Protoplasma, 179:46-60.
13. Hogeteu, T., and Oshima, Y. (1986) Immunofluorescence microscopy of microtubule arrangement in root cells of Pisum sativum L. var Alaska. Plant Cell Physiol., 27:939-945.
14. Lloyd, C.W., Slabas, A.R., Powell, A.J., MacDonald, G., and Badley, R.A. (1979) Cytoplasmic microtubules of higher plant cells visualized with anti-tubulin antibodies. Nature (Lond.), 279:239-241.
15. McCurdy, D.W., Sammut, M., and Gunning, B.E.S. (1988) Immunofluorescent visualization of arrays of transverse cortical actin microfilaments in wheat root-tip cells. Protoplasma, 147:204-206.
16. Osborn, M., and Weber, K. (1982) Immunofluorescence and immunocytochemical procedures with affinity purified antibodies: Tubulin. Methods Cell Biol. 24:97-132.
17. Pickett-Heaps, J.D., and Northcote, D.H. (1966) Organization of microtubules and endoplasmic reticulum during mitosis and cytokinesis in wheat meristems. J. Cell Sci., 1:109-120.
18. Steeves, T.A., and Sussex, I.M. (1972) In: Patterns in Plant Development. Prentice-Hall, Inc., Englewood Cliffs, NJ, pp. 170-190.
19. Tiwari, S.C., Wick, S.M., Williamson, R.E., and Gunning, B.E.S. (1984) The cytoskeleton and integration of cellular function in cells of higher plants. J. Cell Biol., 99:63s-69s.
20. van der Valk, P., Rennie, P.J., Connolly, J.A., and Fowke, L.C. (1980) Distribution of cortical microtubules in tobacco protoplasts. An immunofluorescence microscopic and ultrastructural study. Protoplasma, 105:27-43.
21. Vaughn, K.C., Sherman, T.D., and Renzaglia, K.S. (1993) A centrin homologue is a component of the multilayered structure in bryophytes and pteridophytes. Protoplasma, 175:58-66.
22. Warne, T.R., Warker, G.L., and Hickok, L.G. (1986) A novel method for surface sterilizing fern spores. Am. Fern J., 76:187-188.
23. Weber, K., Bibring, T., and Osborn, M. (1975) Specific visualization of tubulin-containing structures in tissue culture cells by immunofluorescence. Exp. Cell Res., 95:111-120.
24. Wick, S.M. (1993) Immunolabelling of antigens in plant cells. Methods Cell Biol., 37:171-201.
25. Wick, S.M., Seagull, R.W., Osborn, M., Weber, K., and Gunning, B.E.S. (1981) Immunofluorescence microscopy of organized microtubule arrays in structurally stabilized meristematic plant cells. J. Cell Biol., 89:685-690.