Expression, purification, and characterization of recombinant human interleukin-13 from NS-0 cells

Protein Expression and Purification

Volumn 12, Issue 2, 1998, Pages 239-248

  Cannon Carlson, Susan ^a   Varnerin, Jeff ^a   Tsarbopoulos, Anthony ^a   Jenh, Chung Her ^a   Cox, Mary Ann ^a   Chou, Chuan Chu ^a   Connelly, Nancy ^a   Zavodny, Paul ^a   Tang, John C T ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID SEQUENCE; B LYMPHOCYTE; CYTOKINE; DISULFIDE BOND; GEL PERMEATION CHROMATOGRAPHY; GENE EXPRESSION SYSTEM; HYDROXYAPATITE; INTERLEUKIN 13; MACROPHAGE; MASS SPECTROMETRY; MONOCYTE; PURIFICATION; Q SEPHAROSE; RECOMBINANT PROTEIN; SDS POLYACRYLAMIDE GEL ELECTROPHORESIS; T LYMPHOCYTE;

EID: 0032029744     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1997.0835     Document Type: Article

References (37)

1. Minty A., Chalon P., Derocq J.-M., Dumont X., Guillemont J.-C., Kaghad M., Labit C., Leplatois P., Liauzun P., Miloux B., Minty C., Casellas P., Loison G., Lupker J., Shire D., Ferrara P., Caput D. Interleukin-13 is a new human lymphokine regulating inflammatory and immune responses. Nature. 362:1993;248-250.
2. McKenzie A. N. J., Li X., Largaespada D., Sato A., Kaneda A., Zurawski S., Doyle E., Milatovich A., Francke U., Copeland N., Jenkins N., Zurawski G. Structural comparison and chromosomal localization of the human and mouse IL-13 gene. J. Immunol. 150:1993;5346-5444.
3. Magazin M., Guillemot J., Vita N., Ferrara P. Interleukin 13 is a monocyte chemoattractant. Eur. Cytokine Network. 5:1994;397-400.
4. McKenzie A., Culpepper J., De Waal Malefyt R., Briere F., Punnonen J., Aversa G., Sato A., Dang W., Cocks B. G., Menon S., De Vries J., Banchereau J., Zurawski G. Interleukin-13, a T-cell derived cytokine that regulates human monocytes and B-cell function. Immunology. 90:1993;3735-3739.
5. De Vries J., Punnonen J., Cocks B., Aversa G. The role of T/B cell interactions and cytokines in the regulation of human IgE synthesis. Semin. Immunol. 5:1993;431-439.
6. DeFrance T., Carayon P., Billian G., Guillemot J.-C., Minty A., Caput D., Ferrara P. Interleukin-13 is a B cell stimulating factor. J. Exp. Med. 179:1994;135-143.
7. LeGros G., Ben-Saaon S. Z., Seeder R. A., Finkelman F. D., Paul W. J. Exp. Med. 181:1990;537-546.
8. Mikovitz J. A., Meyers A. M., Ortaldo J. R., Minty A., Caput D., Ferrara P., Ruscetti F. W. IL-4 and IL-13 have overlapping but distinct effects on HIV production in monocytes. J. Leukocyte Biol. 56:1994;340-346.
9. Denis M., Ghadirian E. Interleukin 13 and interleukin 4 protect bronchoalveolar macrophages from productive infection with human immunodeficiency virus type I. AIDS Res. Hum. Retroviruses. 10:1994;795-802.
10. De Waal Malefyt R., Figdor C., Huijbnes R., Mohan-Peterson S., Bennett B., Culpepper J., Dang W., Zurawski G., De Vries J. Effects of IL-13 on phenotype, cytokine production and cytotoxic function of human monocytes. J. Immunol. 151:1993;6370-6381.
11. Zurawski G., De Vries J. Interleukin-13, an interleukin-4-like cytokine that acts on monocytes and B cells, but not on T-cells. Immunol. Today. 15:1994;19-26.
12. Morgan J., Dolganov G., Robbins S., Hinton L., Lovett M. The selective isolation of novel cDNAs encoded by the regions surrounding the human interleukin 4 and 5 genes. Nucleic Acids Res. 20:1992;5173-5179.
13. Zurawski G., De Vries J. Interleukin 13 elicits a subset of the activities of its close relative interleukin 4. Stem Cells. 12:1994;169-174.
14. Zurawski S., Vega F. Jr., Huyghe B., Zurawski G. Receptors for interleukin 13 and interleukin 4 are complex and share a novel component that functions in signal transduction. EMBO J. 12:1993;2663-2670.
15. Kimmenade A., Bond M., Schumacher J., Laquoi C., Kastelein R. Expression, renaturation and purification of recombinant human interleukin 4 from Escherichia coli. Eur. J. Biochem. 173:1988;109-114.
16. Trotta P. Physicochemical and structural characteristics of interleukin-4. IL-4 Structure and Function. 1992;CRC Press, Boca Raton. p. 15-32.
17. Bazan J. F. Haemopoietic receptors and helical cytokines. Immunol. Today. 11:1990;350-354.
18. Bazan J. F. Unraveling the structure of IL-2. Science. 257:1992;410-413.
19. Wlodaver A., Pavlovsky A., Gustchina A. Hematopoietic cytokines: Similarities and differences in the structures, with implication for receptor binding. Protein Sci. 2:1993;1373-1382.
20. Wlodaver A., Pavlovsky A., Gustchina A. Crystal structure of human recombinant interleukin 4 at 2.25 angstrom resolution. FEBS Lett. 309:1992;59-64.
21. Bamborough P., Duncan D., Richards W. G. Predictive modeling of the 3-D structure of interleukin 13. Protein Eng. 7:1994;1077-1082.
22. Sambrook J., Fritsch E. F., Maniatis T. Molecular Cloning. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
23. McKenzie A., Culpepper J., de Waal Malefyt R., Briere F., Punnonen J., Aversa G., Sato A., Dang W., Cocks B. G., Menon S., deVries J. E., Banchereau J., Zurawski G. Interleukin-13, a T-cell-derived cytokine that regulates human monocyte and B-cell function. PNAS. 90:1993;3735-3739.
24. Kozak M. An analysis of 5′-non-coding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 15:1987;8125-8148.
25. Akashi M., Shaw G., Hachiya M., Elstner E., Suzuki G., Koeffler P. Number and location of AUUUA motifs: Role in regulating transiently expressed RNAs. Blood. 83:1994;3182-3187.
26. Galfre G., Milstein C. Preparation of monoclonal antibodies: Strategies and procedures. Methods Enzymol. 73:1981;3-46.
27. Bebbington C. R., Renner G., Thomson S., King D., Abrams D., Yarranton G. T. High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker. Bio/Technology. 10:1992;169-175.
28. Patterson M. K. Measurement of growth and viability of cells in culture. Methods Enzymol. 58:1979;141-152.
29. Grasetti D. R., Murray J. F. Jr. Sulfhydryl content determination by use of 4,4′-dithiodipyridine. Arch. Biochem. Biophys. 119:1967;41-49.
30. Tsarbopoulos A., Pramanik B., Labdon J., Reichert P., Giltin G., Patel S., Sardana V., Nagabhushan T. L., Trotta P. Isolation and characterization of a resistant core peptide of recombinant human GM-CSF: confirmation of the GM-CSF amino acid sequence by mass spectrometry. Protein Sci. 2:1993;1948-1958.
31. Siegal J. P., Mostowsky H. A bioassay for the measurement of human interleukin 4. J. Immunol. Methods. 132:1990;287-295.
32. Bhown, A. S. 1990, Handbook of Proteins, 40, 59, A&M Publications, Birmingham, AL.
33. McPherson A. Preparation and Analysis of Protein Crystals. 1982;Wiley, New York. p. 53.
34. Fenn J. B., Mann M., Meng C. K., Wong S. F., Whitehouse C. M. Electrospray ionization - Principles and practice. Mass Spectrom. Rev. 9:1990;37-70.
35. Johnson W. C. Secondary structure of proteins through circular dichroism spectroscopy. Annu. Rev. Biophys. Chem. 17:1988;145-166.
36. Smith L., Redfield C., Boyd J., Lawrence M., Edwards R., Smith R., Dobson C. Human interleukin 4: The solution structure of the four-helix bundle protein. J. Mol. Biol. 224:1992;899-904.
37. Windsor W., Syto R., Le H., Trotta P. Analysis of the conformation and stability ofEscherichia coli. Biochemistry. 30:1991;1259-1264.