Alpha- and beta-scorpion toxins evoke glutamate release from rat cortical synaptosomes with different effects on [Na+](i) and [Ca2+](i)

Neuropharmacology

Volumn 37, Issue 3, 1998, Pages 289-297

  Massensini, André R ^a   Moraes Santos, Tasso ^a   Gomez, Marcus V ^a   Romano Silva, Marco A ^a  

^a FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

Author keywords

Glutamate release; Scorpion toxins; Sodium channels; Synaptosomes

Indexed keywords

CALCIUM ION; GLUTAMATE DEHYDROGENASE; GLUTAMIC ACID; NEUROTRANSMITTER RECEPTOR; SCORPION VENOM; SODIUM; SODIUM CHANNEL;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN CORTEX; BRAIN SYNAPTOSOME; CONTROLLED STUDY; DRUG RECEPTOR BINDING; NEUROPHARMACOLOGY; NEUROTRANSMITTER RELEASE; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALS; CALCIUM; CALCIUM CHANNEL BLOCKERS; CEREBRAL CORTEX; GLUTAMIC ACID; MALE; RATS; SCORPION VENOMS; SODIUM; SYNAPTOSOMES;

EID: 0032029234     PISSN: 00283908     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0028-3908(98)00025-2     Document Type: Article

References (33)

1. + channel α subunit with novel gating properties. Neuron. 1:1988;449-461.
2. Bablito J., Jover E., Couraud F. Activation of the voltage-sensitive sodium channel by a β-scorpion toxin in rat brain nerve-ending particles. J. Neurochem. 46:1986;1763-1770.
3. Barhanin J., Giglio J.R., Schmid A., Sampaio S.V., Lazdunski M. Tityus serrulatus venom contains two classes of toxins. Tityus γ toxin is a new tool with very high affinity for studying the Na channel. J. Biol. Chem. 257:1982;12553-12558.
4. Barhanin J., Pauron D., Lombert A. et al. Electrophysiological characterization solubilization and purification of the Tityus γ toxin receptor associated with the gating components of the Na channel from rat brain. EMBO J. 2:1983;915-920.
5. Broadie K.S. Genetic dissection of the molecular mechanisms of transmitter vesicle release during synaptic transmission. J. Physiol. Paris. 89:1995;59-70.
6. 3H]acetylcholine from brain cortical slices. Neuropharmacology. 34:1995;599-603.
7. Catterall W.A. Cellular and molecular biology of voltage-gated sodium channels. Physiol. Rev. 72:1992;S15-S48.
8. Couraud F., Jover E., Dubois J.M., Rochat H. Two types of toxin receptor sites, one related to the activation, the other to the inactivation of the action potential sodium channel. Toxicon. 20:1982;9-16.
9. +-binding benzofuran isophtalate: the effect of veratridine, ouabain and alpha-latrotoxin. J. Neurochem. 60:1993;1065-1072.
10. Drumond Y.A., Couto A.S., Moraes-Santos T., Almeida A.P., Freire-Maia L. Effects of Ts-γ and tityustoxin purified from Tityus serrulatus scorpion venom on isolated rat atria. Comp. Biochem. Physiol. 111C:1995;183-190.
11. Duncan D.B. Multiple range and multiple T Tests. Biometrics. 11:1955;1-42.
12. Dunkley P.R., Heath J.W., Harrison S.M., Jarvie P.E., Glenfield P.J., Rostas J.A.P. A rapid Percoll gradient procedure for isolation of synaptosomes directly from an S1 fraction: homogeneity and morphology of subcellular fractions. Brain Res. 441:1988;59-71.
13. Dunlap K., Luebke J.L., Turner J.L. Exocytotic calcium channels in mammalian central neurons. Trends Neurosci. 18:1995;89-98.
14. Gautron S., Dos Santos G., Pinto-Henrique D., Koulakoff A., Gros F., Berwald-Netter Y. The glial voltage-gated sodium channel: Cell- and tissue-specific mRNA expression. Proc. Natl. Acad. Sci. USA. 89:1992;7272-7276.
15. Gomez M.V., Diniz C.R. Separation of toxic components from the Brazilian scorpion- Tityus serrulatus-venom. Mem. Inst. Butantan Simp. Int. 33:1966;899-902.
16. Gomez M.V., Romano-Silva M.A., Prado M.A.M. Effects of tityustoxin on central nervous system. J. Toxicol. -Toxin Rev. 14:1995;437-456.
17. 2+ indicators with greatly improved fluorescence properties. J. Biol. Chem. 260:1985;3440-3450.
18. Kayano T., Noda M., Flockerzi V., Takahashi H., Numa S. Primary structure of rat brain sodium channel III deduced from cDNA sequence. FEBS Lett. 228:1988;187-194.
19. Llinás R., Sugimori M., Silver R.B. Microdomains of high calcium concentration in a presynaptic terminal. Science. 256:1992;677-679.
20. + channels. Circulation Res. 80:1997;363-369.
21. 2+. J. Neurochem. 56:1991;86-94.
22. McMahon H.T., Foran P., Dolly J.O., Verhage M., Wiegant V.M., Nicholls D.G. Tetanus toxin and botulinum toxins type A and B inhibit glutamate, γ-aminobutyric acid aspartate and met-enkephalin release from synaptosomes. J. Biol. Chem. 267:1992;21338-21343.
23. Nicholls D.G., Sihra T.S., Sanchez-Prieto J. Calcium-dependent and independent release of glutamate from synaptosomes monitored by continuous fluorometry. J. Neurochem. 49:1987;50-57.
24. Noda M., Ikeda T., Suzuki H., Takeshima H., Takahashi T., Kuno M., Numa S. Expression of functional sodium channels from cloned cDNA. Nature. 322:1986;826-828.
25. Randall A.D., Tsien R.W. Pharmacological dissection of multiple types of calcium channel currents in rat cerebellar neurons. J. Neurosci. 15:1995;2995-3012.
26. Roettger V., Lipton P. Mechanism of glutamate release from rat hippocampal slices during in vivo ischemia. Neuroscience. 75:1996;677-685.
27. 2+ entry linked to rapid glutamate release: studies using the Phoneutria nigriventer toxin PhTX2 and potassium depolarization. Biochem. J. 296:1993;313-319.
28. 2+-dependent glutamate release from synaptosomes. Neurosci. Lett. 169:1994;90-92.
29. Sampaio S.V., Laure C.J., Giglio J.R. Isolation and characterization of toxic proteins from the venom of the Brazilian scorpion Tityus serrulatus. Toxicon. 21:1983;265-277.
30. Schaller K.L., Krzemien D.M., Yarowski P.J., Krueger B.K., Caldwell J.H. A novel abundant sodium channel expressed in neurons and glia. J. Neurosci. 15:1995;3231-3242.
31. Turner TJ, Lampe RA, Dunlap K Characterization of presynaptic calcium channels with omega-Conotoxin MVIIC and omega-grammotoxin SIA: role for a resistant calcium channel type in neurosecretion. Mol. Pharmacol. 47(2):1995;348-353.
32. + channels: a rational and effective strategy against ischemic brain damage. Pharmacol. Rev. 48:1996;21-67.
33. Vijverberg H.P.M., Pauron D., Lazdunski M. The effect of Tityus serrulatus scorpion toxin γ on Na channels in neuroblastoma cells. Pflügers Arch. 401:1984;297-303.