Microscopic Analysis of NADH Fluorescence during Aerobic and Anaerobic Liver Preservation Conditions: A Noninvasive Technique for Assessment of Hepatic Metabolism

Cryobiology

Volumn 36, Issue 2, 1998, Pages 108-114

  Klauke, H ^a   Minor, T ^a   Vollmar, B ^b   Isselhard, W ^a   Menger, M D ^b  

^a UNIVERSITY OF COLOGNE   (Germany)

^b SAARLAND UNIVERSITY   (Germany)

Author keywords

ATP; Cold storage; Fluorescence microscopy; Liver; NADH fluorescence; University of Wisconsin solution

Indexed keywords

ADENOSINE; ALLOPURINOL; GLUTATHIONE; INSULIN; NICOTINAMIDE ADENINE DINUCLEOTIDE; OXYGEN; PRESERVATION SOLUTION; RAFFINOSE; UNIVERSITY OF WISCONSIN LACTOBIONATE SOLUTION; UNIVERSITY OF WISCONSIN-LACTOBIONATE SOLUTION;

AEROBIC METABOLISM; ANAEROBIC GROWTH; ANIMAL; ARTICLE; FLUORESCENCE; LIVER; ORGAN PRESERVATION; RAT; SPRAGUE DAWLEY RAT;

ADENOSINE; AEROBIOSIS; ALLOPURINOL; ANAEROBIOSIS; ANIMALS; FLUORESCENCE; GLUTATHIONE; INSULIN; LIVER; NAD; ORGAN PRESERVATION; ORGAN PRESERVATION SOLUTIONS; OXYGEN; RAFFINOSE; RATS; RATS, SPRAGUE-DAWLEY;

EID: 0032015041     PISSN: 00112240     EISSN: None     Source Type: Journal    
DOI: 10.1006/cryo.1997.2068     Document Type: Article

References (32)

1. Chance, B., and Schoener, B. A correlation of absorption and fluorescence changes in ischemia of the rat liver in vivo. Biochem. Z. 341, 340-345 (1965).
2. Chance, B., and Thorell, B. Localization and kinetics of reduced pyridine nucleotides in living cells by microfluorimetry. J. Biol. Chem. 234, 3044-3050 (1959).
3. Chance, B., Cohen, P., Jobsis, F., and Schoener, B. Intracellular oxidation-reduction states in vivo. The microfluorometry of pyridine nucleotide gives a continuous measurement of the oxidation state. Science 137, 499-508 (1962).
4. Chance, B., Schoener, B., Krejci, K., Russmann, W., Wesemann, W., Schnitger, H., and Bücher, T. Kinetics of fluorescence and metabolite changes in rat liver during a cycle of ischemia. Biochem. Z. 341, 325-333 (1965).
5. Fischer, J. H., Fuchs, M., Miyata, M., and Isselhard, W. Hypothermic liver preservation using different flush solutions and retrograde oxygen persufflation technique. Eur. Surg. Res. 12, 19-20 (1980). [Abstract]
6. Franke, H., Barlow, C. H., and Chance, B. Oxygen delivery in perfused rat kidney: NADH fluorescence and renal functional state. Am. J. Physiol. 231, 1082-1089 (1976).
7. Ince, C., Coremans, J. M. C. C., and Bruining, H. A. In vivo NADH fluorescence. Adv. Exp. Med. Biol. 317, 277-296 (1992).
8. Isselhard, W., and Merguet, H. Metabolite des Glykolysecyklus und des Adenylsäure-Phosphokreatin-Systems im schlagenden und durchbluteten Warmblüterherzen unter verschiedenen Versuchsbedingungen. Pflügers Arch. 276, 211-235 (1962).
9. Lübbers, D., Kessler, M., Scholz, R., and Bucher, Th. Cytochrome reflection spectra and fluorescence of the isolated, perfused, hemoglobin-free rat liver during a cycle of anoxia. Biochem. Z. 341, 346-350 (1965).
10. Menger, M. D., Marzi, I., and Messmer, K. In vivo fluorescence microscopy for quantitative analysis of the hepatic microcirculation in hamsters and rats. Eur. Surg. Res. 23, 158-169 (1991).
11. Minor, T., and Isselhard, W. Venous oxygen insufflation to prevent reoxygenation injury after ischemia of a solid organ. Transplantation 58, 121-123 (1994).
12. Minor, T., and Isselhard, W. Synthesis of high energy phosphates during cold ischemic rat liver preservation with gaseous oxygen insufflation. Transplantation 61, 20-22 (1996).
13. Minor, T., Isselhard, W., and Klauke, H. Reduction in nonparenchymal cell injury and vascular endothelial dysfunction after cold preservation of the liver by gaseous oxygen. Transplant. Int. 9, S425-S428 (1996).
14. Minor, T., Klauke, H., and Isselhard, W. Resuscitation of cadaveric livers from non-heart-beating donors after warm ischemic insult: A novel technique tested in the rat. Experientia 52, 661-664 (1996).
15. Minor, T., Klauke, H., Nagelschmidt, M., and Isselhard, W. Reduction of proteolysis by venous-systemic oxygen persufflation during rat liver preservation and improved functional outcome after transplantation. Transplantation 63, 365-368 (1997).
16. Minor, T., Klauke, H., Vollmar, B., Menger, M. D., and Isselhard, W. Rat liver transplantation after long-term preservation by venous systemic oxygen persufflation. Transplant. Proc. 29, 410-411 (1997).
17. Obi-Tabot, E. T., Hanrahan, L. M., Cachecho, R., Beer, E. R., Hopkins, S. R., Chan, J. C. K., Shapiro, J. M., and LaMorte, W. W. Changes in hepatocyte NADH fluorescence during prolonged hypoxia. J. Surg. Res. 55, 575-580 (1993).
18. Ozaki, N., Tokunaga, Y., Ikai, I., Zaima, M., Sakai, Y., Shimahara, Y., Kamiyama, Y., Yamaoka, Y., Ozawa, K., and Nakase, Y. Pyridine nucleotide fluorometry in preserved porcine liver with fluorocarbon emulsion. Transplantation 48, 198-201 (1989).
19. Renault, G., Raynal, E., Sinet, M., Berthier, J.-P., Godard, B., and Cornillault, J. A laser fluorimeter for cardiac metabolism investigation. Optics Laser Technol. 14, 143-148 (1982).
20. Rolles, K., Foreman, J., and Pegg, D. E. A pilot clinical study of retrograde oxygen persufflation in renal transplantation. Transplantation 48, 339-342 (1989).
21. Schnitger, H., Scholz, R., and Bucher, Th. Comparative fluorometric studies on rat liver in vivo and on isolated, perfused, hemoglobin-free liver. Biochem. Z. 341, 334-339 (1965).
22. Scholz, R., and Bucher, Th. Hemoglobin-free perfusion of rat liver. In "Control of Energy Metabolism" (B. Chance, R. W. Estabrook, and J. R. Williamson, Eds.), pp. 393-435. Academic Press, New York, 1965.
23. Suematsu, M., Suzuki, H., Ishii, H., and Tsuchiya, M. Paradox of oxygen-radical-dependent cell injury in the hypoxic liver microcirculation. Prog. Appl. Microcirc. 19, 127-138 (1993).
24. Thorniley, M. S., Simpkin, S., Fuller, B., Jenabzadeh, M. Z., and Green, C. J. Monitoring of surface mitochondrial NADH levels as an indication of ischemia during liver isograft transplantation. Hepatology 21, 1602-1609 (1995).
25. Tokunaga, Y., Ozaki, N., Wakashiro, S., Ikai, I., Morimoto, T., Shimahara, Y., Kamiyama, Y., Yamaoka, Y., Ozawa, K., and Nakase, Y. Fluorometric study for the noninvasive determination of cellular viability in perfused rat liver. Transplantation 44, 701-706 (1987).
26. Trautschold, I., Lamprecht, W., and Schweitzer, G. ATP: UV-method with hexokinase and glucose-6-phosphate dehydrogenase. In "Methods of Enzymatic Analysis" (H. U. Bergmeyer, Ed.), Vol. 7, pp. 346-357. Verlag Chemie, Weinheim, 1985.
27. Vollmar, B., Burkhardt, M., Minor, T., Klauke, H., and Menger, M. D. High-resolution microscopic determination of hepatic NADH fluorescence for in vivo monitoring of tissue oxygenation during hemorrhagic shock and resuscitation. Microvasc. Res. 54, 164-173 (1997).
28. Vollmar, B., Glasz, J., Post, S., Leiderer, R., and Menger, M. D. Hepatic microcirculatory perfusion failure is a determinant for liver dysfunction in warm ischemia-reperfusion. Am. J. Pathol. 145, 1421-1431 (1994).
29. Vollmar, B., Menger, M. D., Glasz, J., Leiderer, R., and Messmer, K. Impact of leukocyte-endothelial cell interaction in hepatic ischemia/reperfusion injury. Am. J. Physiol. 267, G786-G793 (1994).
30. Weber, D., and Tung, L. C. Konservierung von Schweinelebern durch retrograde Sauerstoff-Persufflation. Z. Exp. Chir. Transplant. Künstl. Organe 19, 217-220 (1986).
31. Wollenberger, A., Ristau, O., and Schoffa, G. Eine einfache Technik der extrem schnellen Abkühlung gröβerer Gewebestücke. Pflügers Arch. 270, 399-412 (1960).
32. Zeintl, H., Tompkins, W. R., Messmer, K., and Intaglietta, M. Static and dynamic microcirculatory video image analysis applied to clinical investigations. Prog. Appl. Microcirc. 11, 1-10 (1986).