Elemental contents in vacuolar granules of ectomycorrhizal fungi measured by EELS and EDXS. A comparison of different methods and preparation techniques

Micron

Volumn 29, Issue 1, 1998, Pages 53-61

  Bucking H ^a   Beckmann, S ^b   Heyser, W ^a   Kottke, I ^b  

^a UNIVERSITY OF BREMEN   (Germany)

^b UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

Ectomycorrhiza; EDXS; EELS; Nitrogen; Phosphorus; Pisolithus tinctorius; Polyphosphate granules; Specimen preparation techniques; Suillus bovinus; Xerocomus badius

Indexed keywords

EID: 0032007794     PISSN: 09684328     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0968-4328(97)00059-0     Document Type: Article

References (43)

1. Agerer, R., 1987-1996. Colour Atlas of Ectomycorrhizae. Einhorn-Verlag, Schwäbisch-Gmünd.
2. Albertano, P., Canini, A. and Caiola, M. G., 1993. Sub-cellular distribution of nitrogen compounds in Azolla and Anabaena by ESI and EELS analysis. Protoplasma, 173, 158-169.
3. Ashford, A. E., Peterson, R. L., Dwarte, D. and Chilvers, G. A., 1986. Polyphosphate granules in eucalypt mycorrhizas: determination by energy dispersive X-ray microanalysis. Can. J. Bot., 64, 677-687.
4. Brun, A., Chalot, M., Duponnois, R., Botton, B. and Dexheimer, J., 1994. Immunogold localisation of glutamine synthetase (GS) and NADP-glutamate dehydrogenase of Laccaria laccata in Douglas fir ectomycorrhizas. Mycorrhiza, 5, 139-144.
5. Bucking, H., 1995. Untersuchungen zur Aufnahme, Speicherung und Verteilung von Phosphor in unmykorrhizierten und mykorrhizierten Sämlingen von Pinus sylvestris L. Ph.D. thesis, University of Bremen, Germany.
6. Bucking, H. and Heyser, W., 1997. Intracellular compartmentation of phosphorus in roots of Pinus sylvestris L. and the implications for transfer processes in ectomycorrhizae. In: Trees - Contributions to Modern Tree Physiology, eds H. Rennenberg, W. Eschrich and H. Ziegler. SPB Academic Publ., The Hague.
7. Cox, G., Sanders, F. E., Tinker, P. B. and Wild, J. A., 1975. Ultrastructural evidence relating to host-endophyte transfer in a vesicular-arbuscular mycorrhiza. In: Endomycorrhizas, eds F. E. Sanders, B. Mosse and P. B. Tinker, pp. 297-312. Academic Press, New York.
8. Cramer, C. L. and Davis, R. H., 1984. Polyphosphate-cation interaction in the amino acid-containing vacuole of Neurospora crassa. J. Biol. Chem., 259, 5152-5157.
9. Cramer, C. L., Vaughn, L. E. and Davis, R. H., 1980. Basic amino acids and inorganic polyphosphates in Neurospora crassa: independent regulation of vacuolar pools. J. Bacteriol., 142, 945-952.
10. Doonan, B. B., Crang, R. E., Jensen, T. E. and Baxter, M., 1979. In situ X-ray energy dispersive microanalysis of polyphosphate bodies in Aureobasidium pullulans. J. Ultrastruct. Res., 69, 232-238.
11. Franz, F. and Acker, G., 1995. Rhizomorphs of Picea abies ectomycorrhizae: ultrastructural aspects and elemental analysis (EELS and ESI) on hyphal inclusions. Nova Hedwigia, 60, 253-267.
12. Fritz, E., 1980. Microautoradiographic localization of assimilates in phloem: problems and new method. Ber. Dtsch. Bot. Ges., 93, 109-121.
13. Fritz, E., 1989. X-ray microanalysis of diffusible elements in plant cells after freeze-drying, pressure infiltration with ether and embedding in plastic. Scanning Microsc., 3, 517-526.
14. 31P-NMR study of meta-chromatic granules in birch mycorrhizas. New Phytol., 112, 49-54.
15. Hall, T. A. and Gupta, B. L., 1984. The application of EDXS to the biological sciences. J. Microsc., 136, 193-208.
16. Haug, L. and Pritsch, K., 1992. Ectomycorrhizal Types of Spruce (Picea abies (L.) Karst.) in the Black Forest. A Microscopical Atlas. Kernforschungszentrum, Karlsruhe.
17. Hofer, F. and Golob, P., 1987. New examples for near-edge fine structures in electron energy loss spectroscopy. Ultramicroscopy, 21, 379-384.
18. Karnovsky, M. J., 1965. A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell. Biol., 27, 137A-138A.
19. Klionsky, D. J., Herman, P. K. and Emr, S. D., 1990. The fungal vacuole: composition, function, and biogenesis. Microbiological Reviews, 54, 266-292.
20. Kottke, I., Guttenberger, M., Hampp, R. and Oberwinkler, S., 1987. An in vitro method for establishing mycorrhizae on coniferous tree seedlings. Trees, 1, 191-194.
21. Kottke, I., Holopainen, T., Alanen, E. and Tumau, K., 1995. Deposition of nitrogen in vacuolar bodies of Cenococcum geophilum Fr. mycorrhizas as detected by electron energy loss spectroscopy. New Phytol., 129, 411-416.
22. Kulaev, I. S., 1979. The Biochemistry of Inorganic Polyphosphates. J. Wiley, Chichester.
23. Leapman, R. D. and Hunt, J. A., 1991. Comparison of detection limits for EELS and EDXS. Microsc. Microanal. Microstruct., 2, 231-244.
24. 2+ and orthophosphate ions in cytoplasm of the yeast Saccharomyces carlsbergensis. Arch. Microbiol., 132, 289-293.
25. 31P nuclear magnetic resonance study. Planta, 194, 241-246.
26. Messenguy, F., 1987. Multiplicity of regulatory mechanisms controlling amino acid biosynthesis in Saccharomyces cerevisiae. Microbiological Sciences, 4, 150-153.
27. Miller, J. J., 1994. In vitro experiments concerning the state of polyphosphate in the yeast vacuole. Can. J. Microbiol., 30, 236-246.
28. Molina, R. and Palmer, J. G., 1982. Isolation, maintenance, and pure culture manipulation of ectomycorrhizal fungi. In: Methods and Principles of Mycorrhizal Research, ed. N. C. Schenck, pp. 115-129. The American Phytopathological Society, St. Paul.
29. Okorokov, L. A., Lichko, L. P. and Kulaev, I. S., 1980. Vacuoles: main compartments of potassium, magnesium, and phosphate ions in Saccharomyces carlsbergensis cells, J. Bacteriol., 144, 661-665.
30. Orlovich, D. A. and Ashford, A. E., 1993. Polyphosphate granules are an artefact of specimen preparation in the ectomycorrhizal fungus Pisolithus tinctorius. Protoplasma, 173, 91-102.
31. Orlovich, D. A., Ashford, A. E. and Cox, G. C., 1989. A reassessment of polyphosphate granule composition in the ectomycorrhizal fungus Pisolithus tinctorius. Aust. J. Plant. Physiol., 16, 107-115.
32. Peterson, R. L. and Howarth, M. J., 1991. Morphometric and energy dispersive X-ray analysis of polyphosphate distribution in the VAM fungus Glomus versiforme associated with leek. Symbiosis, 11, 63-71.
33. Plattner, H. and Zingsheim, H. P., 1987. Elektronenmikroskopische Methodik in der Zell- und Molekularbiologie, pp. 163-176. Gustav Fischer, Stuttgart.
34. Robinson, D. G., Ehlers, U., Herken, R., Herrmann, B., Mayer, F. and Schürmann, F.-W., 1985. Präparationsmethodik in der Elektronenmikroskopie, pp. 134-135. Springer Verlag.
35. Rosenberg, H., 1966. The isolation and identification of volutin granules from Tetrahymenta. Exp. Cell Res., 41, 397-410.
36. Spurr, A. R., 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res., 26, 31-43.
37. Stelzer, R. and Lehmann, H., 1993. Recent developments in electron microscopical techniques for studying ion localization in plant cells. Plant Soil, 155/156, 33-43.
38. Van Steveninck, R. F. M. and Van Steveninck, M. E., 1991. Microanalysis. In: Electron Microscopy of Plant Cells, eds J. E. Hall and C. Hawes, pp. 415-455. Academic Press, London.
39. Strullu, D. G., Gourret, J. P., Garrec, J. P. and Fourcy, A., 1981. Ultrastructure and electron-probe microanalysis of the meta-chromatic vacuolar granules occuring in Taxus mycorrhizas. New Phytol., 87, 537-545.
40. Turnau, K., Kottke, I. and Oberwinkler, F., 1993. Element localization in mycorrhizal roots of Pteridium aquilinum (L.) Kuhn collected from experimental plots treated with cadmium dust. New Phytol., 123, 313-324.
41. Turnau, K., Kottke, I. and Oberwinkler, F., 1993. Paxillus involutus-Pinus sylvestris mycorrhizae from heavily polluted forest. I. Element localization using electron energy loss spectroscopy and imaging. Bot. Acta, 106, 213-219.
42. Urech, K., Dürr, M., Boller, T. and Wiemken, A., 1978. Localization of polyphosphate in vacuoles of Saccharomyces cerivisiae. Arch. Microbiol., 116, 275-278.
43. White, J. A. and Brown, M. F., 1979. Ultrastructure and X-ray analysis of phosphorus granules in a vesicular-arbuscular mycorrhizal fungus. Can. J. Bot., 57, 2812-2818.