Thermostable variants of Zymomonas mobilis alcohol dehydrogenase obtained using PCR-mediated random mutagenesis

Protein Expression and Purification

Volumn 12, Issue 1, 1998, Pages 61-66

  Rellos, Peter ^a   Pinheiro, Leonardo ^a   Scopes, Robert K ^a  

^a LA TROBE UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL DEHYDROGENASE; FERROUS ION; HALF LIFE TIME; MUTAGENESIS; MUTATION; PH; POLYMERASE CHAIN REACTION; THERMOSTABILITY; ZYMOMONAS MOBILIS;

EID: 0032006473     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1997.0815     Document Type: Article

References (18)

1. Spee J. H., de Vos W. M., Kuipers O. P. Efficient random mutagenesis method with adjustable mutation frequency by use of PCR with dITP. Nucleic Acids Res. 21:1993;777-778.
2. Moran G. R., Entsch B. Plasmid mutagenesis by PCR for high-level expression of parahydroxybenzoate hydroxylase. Protein Express. Purif. 6:1995;164-168.
3. Chen K., Arnold F. H. Enzyme engineering for nonaqueous solvents: random mutagenesis to enhance activity of subtilisin E in polar organic media. Bio. 9:1991;1073-1077.
4. Rellos P., Scopes R. K. Polymerase chain reaction-based random mutagenesis: Production and characterisation of thermostable mutantsof Zymomonas mobilis. Protein Express. Purif. 5:1994;270-277.
5. Rellos P., Schwindt B., Scopes R. K. Useful mutants ofZymomonas mobilisin. 1995;Plenum, New York. p. 435-440.
6. Stemmer W. P. C. Rapid evolution of a protein in vitro by DNA shuffling. Nature. 370:1994;389-391.
7. Nosoh Y., Sekiguchi T. Protein thermostability: Mechanism and control through protein engineering. Biocatalysis. 1:1988;257-273.
8. Pjura P., Matsumura M., Baase W. A., Matthews B. W. Development of anin vivo. Protein Sci. 2:1993;2217-2225.
9. Ingram L. O., Conway T., Clark D. P., Sewell G. W., Preston J. F. Genetic engineering of ethanol production inEscherichia coli. Appl. Microbiol. Biotechnol. 53:1987;2420-2425.
10. Ingram L. O., Doran J. B. Conversion of cellulosic materials to ethanol. FEMS Microbiol. Rev. 16:1995;235-241.
11. Neale A. D., Scopes R. K., Kelly J. M. Alcohol production from glucose and xylose usingEscherichia coliZymomonas mobilis. Appl. Microbiol. Biotechnol. 29:1988;162-167.
12. Neale A. D., Scopes R. K., Kelly J. M., Wettenhall R. E. HR. E. The two alcohol dehydrogenases fromZymomonas mobilis. Eur. J. Biochem. 154:1986;119-124.
13. Rellos P., Ma J., Scopes R. Alteration of substrate specificity ofZymomonas mobilisin vitro. Protein Express. Purif. 9:1997;83-90.
14. Fromant M., Blanquet S., Plateau P. Direct random mutagenesis of gene-sized DNA fragments using polymerase chain reaction. Anal. Biochem. 224:1995;347-353.
15. Nagao T., Makiro Y., Yamamoto K., Urabe I., Okada H. Stability-increasing mutants of glucose dehydrogenase. FEBS Lett. 253:1989;113-116.
16. Zulli F., Schneiter R., Urfer R., Zuber H. Engineering thermostability and activity of lactate dehydrogenases from bacilli. Biol. Chem. Hoppe Seyler. 372:1991;363-372.
17. 2+Escherichia coliZymomonas mobilis. J. Biol. Chem. 269:1994;6592-6597.
18. Reid M. F., Fewson C. A. Molecular characterization of microbial alcohol dehydrogenases. Crit. Rev. Microbiol. 20:1994;13-56.