Structural characterization of recombinant hepatitis E virus ORF2 proteins in baculovirus-infected insect cells

Protein Expression and Purification

Volumn 12, Issue 1, 1998, Pages 75-84

  Robinson, Robin A ^a   Burgess, Wilson H ^e   Emerson, Suzanne U ^b,c   Leibowitz, Rande S ^a   Sosnovtseva, Svetlana A ^a   Tsarev, Sergei ^d   Purcell, Robert H ^b,c  

^a Div of Molec Virol and Immunology   (United States)

^b HOLLAND LABORATORY   (United States)

^c NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^d WALTER REED ARMY INSTITUTE OF RESEARCH   (United States)

^e NONE

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID ANALYSIS; ANION EXCHANGE; ANTIGEN; BACULOVIRUS; CHROMATOGRAPHY; GEL FILTRATION; GENE EXPRESSION KINETICS; HEPATITIS VIRUS; INSECT CELL; MASS SPECTROMETRY; MOLECULAR WEIGHT; POLYPEPTIDE; PROTEIN PRODUCT; PURIFICATION; RECOMBINANT PROTEIN; RESIDUE; SEQUENCE ANALYSIS;

EID: 0032005693     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1997.0817     Document Type: Article

References (22)

1. Bradley D. W., Krawczynski K., Cook E. H. Jr., McCaustland K. A., Humphrey C. D., Spelbring J. E., Myint H., Maynard J. E. Enterically transmitted non-A, non-B hepatitis: Serial passage of disease in cynomolgus monkeys and tamarins and recovery of disease-associated 27 to 34-nm virus-like particles. Proc. Natl. Acad. Sci. USA. 84:1987;6277-6281.
2. Purcell R. H., Ticehurst J. R. Enterically transmitted non-A non-B hepatitis: Epidemiology and clinical characteristics. Viral Hepatitis and Liver Disease. 1988;A. R. Liss, New York. p. 131-137.
3. Bradley D. W. Enterically-transmitted non-A, non-B hepatitis. Br. Med. Bull. 46:1990;442-461.
4. Ticehurst J. R. Identification and characterization of hepatitis E virus. Hollinger F. R., Lemon S. M., Margolis H. S. Viral Hepatitis and Liver Disease. 1991;501-513 Williams & Wilkins, Baltimore.
5. Tam A. W., Smith M. M., Guerra M. E., Huang C.-C., Bradley D. W., Fry K. E., Reyes G. R. Hepatitis E virus (HEV): Molecular cloning and sequencing of the full-length viral genomes. Virology. 185:1991;120-131.
6. Tsarev S. A., Emerson S. U., Reyes G. R., Tsareva T. S., Legters L. J., Malik I. A., Iqbal M., Purcell R. H. Characterization of a prototype strain of hepatitis E virus. Proc. Natl. Acad. Sci. USA. 89:1992;559-563.
7. Huang C.-C., Nguyen D., Fernandez J., Yun K. Y., Fry K. E., Bradley D. W., Tam A. W., Reyes G. R. Molecular cloning and sequencing of the Mexico isolate of hepatitis E virus (HEV). Virology. 191:1992;550-558.
8. Aye T. T., Uchida T., Ma X.-Z., Iida F., Shikata T., Zhuang H., Win K. M. Complete nucleotide sequence of a hepatitis E virus isolated from the Xinjiang epidemic (1986-1988) of China. Nucleic Acids Res. 20:1992.
9. Bi S.-L., Purdy M. A., McCaustland K. A., Margolis H. S., Bradley D. W. The sequence of hepatitis E virus isolated directly from a single source during an outbreak in China. Virus Res. 28:1993;233-247.
10. Aye T. T., Uchida T., Ma X.-Z., Iida F., Shikata, Ichikawa M., Rikihisa T., Win K. M. Sequence and gene structure of the hepatitis E virus isolated from Myanmar. Virus Genes. 7:1993;95-110.
11. Yin S, Purcell R. H., Emerson S. U. A new Chinese isolate of hepatitis E virus: Comparison with stains recovered from different geographical regions. Virus Genes. 9:1994;23-32.
12. Jameel S., Zafrullah M., Ozdener M. H., Panda S. K. Expression in animal cells and characterization of the hepatitis E virus structural elements. J. Virol. 70:1996;207-216.
13. Ho J., Tam A. W., Yarbough P. O., Reyes G. R., Carl M. Expression and diagnostic utility of hepatitis E virus putative structural proteins expressed in insect cells. J. Clin. Microbiol. 31:1993;2167-2174.
14. McAtee C. P., Zhang Y., Yarbough P., Fuerst T. R., Stone K. L., Samander S., Williams K. Purification and characterization of a hepatitis E vaccine protein candidate by liquid chromatography-mass spectrometry. J. Chromatogr. B. 685:1996;91-104.
15. McAtee C. P., Zhang Y., Yarbough P., Bird T., Fuerst T. R. Purification of a soluble hepatitis E open reading frame 1-derived protein with unique antigenic properties. Protein Express. Purif. 8:1996;262-270.
16. Tsarev S. A., Tsareva T. S., Emerson S. U., Kapikian A. Z., Ticehurst J., London W., Purcell R. H. ELISA for antibody to hepatitis E virus (HEV) based on complete open reading frame-2 protein expressed in insect cells: Identification of HEV infection in primates. J. Infect. Dis. 168:1993;369-378.
17. Tsarev S. A., Tsareva T. S., Emerson S. U., Govindarajan S., Shapiro M., Gerin J. L., Robinson R., Gorbalenya A. E., Purcell R. H. Prospects for prevention of hepatitis E. Buisson Y., Coursaget P., Kane M. Enterically Transmitted Hepatitis Viruses. 1996;373-383 Joueles ToursLa Simarre.
18. Luckow V. A., Lee S. L., Barry G. F., Olin P. O. Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated inEscherichia coli. J. Virol. 67:1993;4566-4579.
19. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
20. Abersold R. H., Leavitt J., Saavedra R. A., Hood L. E., Kent S. B. H. Internal amino acid sequence analysis of proteins separated by one-or two-dimensional gel electrophoresis afterin situ. Proc. Natl. Acad. Sci. USA. 84:1987;6970-6974.
21. Tsarev S. A., Tsareva T. S., Emerson S. U., Govindarajan S., Shapiro M., Gerin J. L., Purcell R. H. Successful passive and active immunization of cynomolgus monkeys against hepatitis E. Proc. Natl. Acad. Sci. USA. 91:1994;10198-10202.
22. Tsarev S. A., Tsareva T. S., Emerson S. U., Yarbough P. O., Legters L. J., Moskal T., Purcell R. H. Infectivity titration of a prototype strain of hepatitis E virus in cynomolgus monkeys. J. Med. Virol. 43:1994;135-142.