Structural investigation of the exopolysaccharide produced by Pseudomonas flavescens strain B62 - Degradation by a fungal cellulase and isolation of the oligosaccharide repeating unit

European Journal of Biochemistry

Volumn 251, Issue 3, 1998, Pages 971-979

  Cescutti, Paola ^a   Toffanin, Renato ^b   Fett, William F ^c   Osman, Stanley F ^c   Pollesello, Piero ^d   Paoletti, Sergio ^a  

^a UNIVERSITY OF TRIESTE   (Italy)

^b INFN Area di Ricerca Trieste   (Italy)

^c EASTERN REGIONAL RESEARCH CENTER   (United States)

^d ORION CORPORATION   (Finland)

Author keywords

Cellulase; Exopolysaccharide; NMR; Pseudomonas; Structure

Indexed keywords

CELLULASE; POLYSACCHARIDE;

ARTICLE; BACTERIUM ISOLATION; ENZYME DEGRADATION; GLYCOCALYX; METHYLATION; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; OPPORTUNISTIC INFECTION; PRIORITY JOURNAL; PROTEIN ISOLATION; SEQUENCE HOMOLOGY; STOICHIOMETRY;

EID: 0032005234     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2510971.x     Document Type: Article

References (41)

1. Albersheim, P., Nevins, D. J., English, P. D. & Karr, A. (1967) A method for the analysis of sugars in plant cell-wall polysaccharides by gas-liquid chromatography, Carbohydr. Res. 5, 340-345.
2. Aman, P., Franzèn, L.-E., Darwill, J. E., McNeil, M., Darvill, A. G. & Albersheim, P. (1982) The structure of the acidic polysaccharide secreted by Rhizobium phaseoli strain 127 K38, Carbohydr. Res. 103, 77-100.
3. Amemura, A., Harada, T., Abe, M. & Higashi, S. (1983) Structural studies on the extracellular acidic polysaccharide from Rhizobium trifolii 4S, Carbohydr. Res. 115, 165-174.
4. 13C assignments from sensitivity-enhanced detection of heteronuclear multiple-bond connectivity by 2D multiple quantum NMR, J. Am. Chem. Soc. 108, 2093-2094.
5. Boccara, M., Aymeric, J. & Camus, C. (1994) Role of endoglucanases in Erwinia chrysanthemi 3937 virulence on Saintpaulia ionantha, J. Bacteriol. 176, 1524-1526.
6. 13C-H coupling constants, Tetrahedron Lett. 13, 1037-1040.
7. Bodenhausen, G. & Ruben, D. J. (1980) Natural abudance nitrogen-15 NMR by enhanced heteronuclear spectroscopy, Chem. Phys. Lett. 69, 185-189.
8. Chowdhury, T. A., Lindberg, B., Lindquist, U. & Baird, J. (1987) Structural studies of an extracellular polysaccharide (S-198) elaborated by Alcaligenes ATCC 31853, Carbohydr. Res. 161, 127-132.
9. Costerton, J. W., Lewandowski, Z., Caldwell, D. E., Korber, D. R. & Lappin-Scott, H. M. (1995) Microbial biofilms, Annu. Rev. Microbiol. 49, 711-745.
10. Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. & Smith, F. (1956) Colorimetric method for determination of sugars and related substances, Anal. Chem. 28, 350-356.
11. Fett, W. F. (1993) Bacterial exopolysaccharides: their nature, regulation and role in host-pathogen interactions, Curr. Top. Bot. Res. 1, 367-390.
12. Fett, W. F., Osman, S. F., Wijey, C. & Singh, S. (1993) Exopolysaccharides of rRNA group I pseudomonads, in Carbohydrates and carbohydrate polymers (Yalpani, M., ed.) pp. 52-61, ATL Press, Inc., Mt Prospect IL.
13. Fett, W. F., Cescutti, P. & Wijey, C. (1996) Exopolysaccharides of the plant pathogens Pseudomonas corrugata and Ps. flavescens and the saprophyte Ps. chlororaphis, J. Appl. Bacteriol. 81, 181-187.
14. Gacesa, P. & Russell, N. J. (1990) Pseudomonas infection and alginates. Biochemistry, genetics and pathology, Chapman and Hall, London.
15. Garozzo, D., Impallomeni, G., Spina, E., Sturiale, L., Cesàro, A. & Ceseutti, P. (1995) Identification of N-acetylglucosamine and 4-O-[1-carboxyethyl]mannose in the exopolysaccharide from Cyanospira capsulata, Carbohydr. Res. 270, 97-106.
16. Geyer, H., Himmelspach, K., Kwiatkowski, B., Schlecht, S. & Stirm, S. (1983) Degradation of bacterial surface carbohydrates by virus-associated enzymes, Pure Appl. Chem. 55, 637-653.
17. Gerwig, G. J., Kamerling, J. P. & Vliegenthart, J. F. G. (1978) Determination of the D and L configuration of neutral monosaccharides by high-resolution capillary G.L.C., Carbohydr. Res. 62, 349-357.
18. Gerwig, G. J., Kamerling, J. P. & Vliegenthart, J. F. G. (1979) Determination of the absolute configuration of monosaccharides in complex carbohydrates by capillary G.L.C., Carbohydr. Res. 77, 1-7.
19. Gough, C. L., Dow, J. M., Barber, C. E. & Daniels, M. J. (1988) Cloning of two endoglucanase genes of Xanthomonas campestris pv. campestris: analysis of the role of the major endoglucanase in pathogenesis, Mol. Plant-Microbe Interact. 1, 275-281.
20. Hakomori, S. (1964) A rapid permethylation of glycolipid, and polysaccharide catalyzed by methylsulfinyl carbanion in dimethyl sulfoxide, J. Biochem. (Tokyo) 55, 205-208.
21. Hildebrand, D. C., Palleroni, N. J., Hendson, M., Toth, J. & Johnson, J. L. (1994) Pseudomonas flavescens sp. nov., isolated from walnut blight cankers, Int. J. System. Microbiol. 44, 410-415.
22. Hough, L. & Theobald, R. S. (1963) De-O-methylation with hydrobromic acid and boron tribromide, Methods Carbohydr. Chem. 2, 203-206.
23. Hurd, R. E. & John, B. K. (1991) Gradient-enhanced proton-detected heteronuclear multiple-quantum coherence spectroscopy, J. Magn. Reson. 91, 648-653.
24. Iansson, P.-E., Lindberg, B., Lönngren, J., Ortega, C. & Nimmich, W. (1984) Structural studies of the capsular polysaccharide of Klebsiella type 66, Carbohydr. Res. 132, 297-305.
25. Iansson, P.-E., Lindberg, B., Widmalm, G. & Sandford, P. A. (1985) Structural studies of an extracellular polysaccharide (S-130) elaborated by Alcaligenes ATCC 31555, Carbohydr. Res. 139, 217-223.
26. Iansson, P.-E., Kumar, N. S. & Lindberg, B. (1986) Structural studies of a polysaccharide (S-88) elaborated by Pseudomonas ATCC 31554, Carbohydr. Res. 156, 165-172.
27. Kenne, L. & Lindberg, B. (1983) Bacterial polysaccharides, in The polysaccharides (Aspinall, G. O., ed.) vol. 2, pp. 287-363, Academic Press, New York.
28. Kochetkov, N. K., Sviridov, A. F., Arifkhodzhaev, K. A., Chizhov, O. S. & Shashkov, A. S. (1979) The structure of the extracellular polysaccharide from Mycobacterium lacticolum strain 121, Carbohydr. Res. 71, 193-203.
29. Leigh, J. A. & Coplin, D. L. (1992) Exopolysaccharides in plant-bacterial interactions, Annu. Rev. Microbiol. 46, 307-346.
30. Lindberg, B., Lindqvist, B. & Lönngren, J. (1976) 4-O-[(S)-1-Carboxyethyl]-D-glucuronic acid: a component of the Klebsiella type 37 capsular polysaccharide, Carbohydr. Res. 49, 411-417.
31. Osman, S. F. & Fett, W. F. (1993) Structure of the acidic exopolysaccharide of Pseudomonas marginalis strain ATCC 10844, Carbohydr. Res. 242, 271-275.
32. Osman, S. F., Fett, W. F. & Dudley, R. L. (1994) Structure of the exopolysaccharide of Pseudomonas stutzeri strain ATCC 17588, Carbohydr. Res. 265, 319-322.
33. Palleroni, N. J. (1993) Pseudomonas classification: a new case history in the taxonomy of Gram-negative bacteria, Antonie Leeuwenhoek 64, 231-251.
34. Parolis, L. A., Parolis, H., Niemann, H. & Stirm, S. (1988) Primary structure of Klebsiella serotype K22 capsular polysaccharide: another glycan containing 4-O-[(S)-1-carboxyethyl]-D-glucuronic acid, Carbohydr. Res. 179, 301-314.
35. Phillips, L. R. & Fraser, B. A. (1981) Methylation of carbohydrates with dimsyl potassium in dimethyl sulfoxide, Carbohydr. Res. 90, 149-152.
36. Pons, J. L., Malliavin, T. E. & Delsuc, M. A. (1996) Gifa V4: a complete package for the NMR data-set processing, J. Biomol. NMR 8, 445-452.
37. Prehm, P. (1980) Methylation of carbohydrates by methyl trifluoromethanesulfonate in trimethyl phosphate, Carbohydr. Res. 78, 372-374.
38. Roberts, D. P., Denny, T. P. & Schell, M. A. (1988) Cloning of the egl gene of Pseudomonas solanacearum and analysis of its role in phytopathogenicity, J. Bacteriol. 170, 1445-1451.
39. Sanford, P. A. & Baird, J. (1983) Industrial utilization of polysaccharides, in The polysaccharides (Aspinall, G. O., ed.) vol. 2, pp. 411-485. Academic Press, New York.
40. Sweet, D. P., Shapiro, R. H. & Albersheim, P. (1975) Quantitative analysis by various g.l.c. response-factor theories for partially methylated and partially ethylated alditol acetates, Carbohydr. Res. 40, 217-225.
41. Taylor, R. L. & Conrad, H. E. (1972) Stoichiometric depolymerization of polyuronides and glycosaminoglycuronans to monosaccharides following reduction of their carbodiimide-activated carboxyl groups, Biochemistry 11, 1383-1388.