Identification of structural elements of subunit β of human protein kinase CK2 participating in tight physical α-β intersubunit contacts directly adjacent to a surface-oriented region

European Journal of Biochemistry

Volumn 251, Issue 3, 1998, Pages 667-672

  Krehan, Andreas ^a   Meggio, Flavio ^b   Pipkorn, Rüdiger ^a   Pinna, Lorenzo A ^b   Pyerin, Walter ^a  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^b UNIVERSITY OF PADOVA   (Italy)

Author keywords

Holoenzyme structure; Human; Intersubunit contact; Protein kinase CK2

Indexed keywords

CASEIN KINASE II;

ARTICLE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME SUBUNIT; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; BINDING SITES; BLOTTING, WESTERN; CASEIN KINASE II; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; MACROMOLECULAR SUBSTANCES; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PROTEIN CONFORMATION; PROTEIN-SERINE-THREONINE KINASES; RECOMBINANT PROTEINS;

EID: 0032005135     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2510667.x     Document Type: Article

References (30)

1. Allende, J. E. & Allende, C. C. (1995) Protein kinase CK2: an enzyme with multiple substrates and a puzzling regulation, FASEB J. 9, 313-323.
2. Antonelli, M., Daniotti, J. L., Rojo, D., Allende, C. C. & Allende, J. E. (1996) Cloning, expression and properties of the α′ subunit of casein kinase 2 from zebrafish (Danio rerio), Eur. J. Biochem. 241, 272-279.
3. Bodenbach, L., Fauss, J., Robitzki, A., Krehan, A., Lorenz, P., Lozeman, F. J. & Pyerin, W. (1994) Recombinant human casein kinase II. Eur. J. Biochem. 220, 263-274.
4. Boldyreff, B., Meggio, F., Pinna, L. A. & Issinger, O.-G. (1992) Casein kinase-2 structure-function relationship: creation of a set of mutants of the β subunit that variably surrogate the wildtype β subunit function, Biochem. Biophys. Res. Commun. 188, 228-234.
5. Boldyreff, B., Meggio, F., Pinna, L. A. & Issinger, O.-G. (1993) Reconstitution of normal and hyperactivated forms of casein kinase-2 by variably mutated β-subunits, Biochemistry 32, 12672-12677.
6. Boldyreff, B., Meggio, F., Pinna, L. A. & Issinger, O.-G. (1994) Reconstitution of normal and hyperactivated forms of casein kinase-2 by variably mutated β-subunits, Cell. Mol. Biol. Res. 40, 391-399.
7. Bozzetti, M. P., Massari, S., Finelli, P., Meggio, F., Pinna, L. A., Boldyreff, B., Issinger, O.-G., Palumbo, G., Ciriaco, C., Bonaccorsi, S. & Pimpinelli, S. (1995) The Ste locus, a component of the parasitic cry-Ste system of Drosophila melanogaster, encodes a protein that forms crystals in primary spermatocytes and mimics properties of casein kinase 2, Proc. Natl Acad. Sci. USA 92, 6067-6071.
8. Chester, M., Yu, I. J. & Marshak, D. R. (1995) Identification and characterization of protein kinase CKII isoforms in HeLa cells. J. Biol. Chem. 270, 7501-7514.
9. Clark, A. C., Hugo, E. & Frieden, C. (1996) Determination of regions in the dihydrofolate reductase structure that interact with the molecular chaperonin GroEI, Biochemistry 35, 5893-5901.
10. Gietz, R. D., Graham, K. C. & Litchfield, D. W. (1995) Interactions between the subunits of casein kinase II, J. Biol. Chem. 270, 13017-13021.
11. Cochet, C. & Chambaz, E. M. (1983) Oligomeric structure and catalytic activity of G-type casein kinase, J. Biol. Chem. 258, 1403-1406.
12. Glover, C. V. C. (1998) On the physiological role of casein kinase II in Saccharomyces cerevisiae, Prog. Nucleic Acid Res. Mol. Biol. 59, 95-133.
13. Hinrichs, M. V., Gatica, M., Allende, C. C. & Allende, J. E. (1995) Site-directed mutants of the β subunit of protein kinase CK2 demonstrate the important role of Pro-58, FEBS Lett. 368, 211-214.
14. Issinger, O.-G. (1993) Casein kinases: pleiotropic mediators of cellular regulation, Pharmac. Ther. 59, 1-30.
15. Krehan, A., Lorenz, R., Plana-Coll, M. & Pyerin, W. (1996) Interaction sites between catalytic and regulatory subunits in human protein kinase CK2 holoenzymes as indicated by chemical cross-linking and immunological investigations, Biochemistry 35, 4966-4975.
16. Kobayashi, R. & Tashima, Y. (1989) Visualization of antigen on nitrocellulose membrane by the oxidative coupling reaction of N,N′-dimethyl-p-phenylendiamine and 4-chloro-1-naphthol. Anal. Biochem. 183, 9-12.
17. Kusk, M., Bendixen, C., Duno, M., Westergaard, O. & Thomsen, B. (1995) Genetic dissection of intersubunit contacts within human protein kinase CK2, J. Mol. Biol. 253, 703-711.
18. Kyhse-Andersen, J. (1984) Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamid to nitrocellulose. J. Biochem. Biophys. Methods 10, 203-209.
19. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
20. Litchfield, D. W. & Lüscher, B. (1993) Casein kinase II in signal transduction and cell cycle regulation. Mol. Cell. Biochem. 127/128. 187-199.
21. Litchfield, D. W., Slominski, E., Lewenza, S., Narvey, M., Bosc, D. G. & Gietz, R. D. (1996) Analysis of interactions between the subunits of protein kinase CK2, Biochem. Cell. Biol. 74, 541-547.
22. Marin, O., Meggio, F., Boldyreff, B., Marin, O., Issinger, O.-G. & Pinna, L. A. (1995) Dissection of the dual function of the β-suhunit of protein kinase CK2 ('casein kinase 2'): a synthetic peptide reproducing the carboxyl-terminal domain mimicks the positive but not the negative effects of the whole protein. FEBS Lett. 363, 111-114.
23. Marin, O., Meggio, F., Sarno, S. & Pinna, L. A. (1997) Physical dissection of the structural elements responsible for regulatory properties and intersubunitary interactions of protein kinase CK2-β-subunit, Biochemistry 36, 7192-7198.
24. Meggio, F., Boldyreff, B., Marin, O., Pinna, L. A. & Issinger, O.-G. (1992) Role of the β subunit of casein kinase-2 on the stability and specificity of the recombinant reconstituted holoenzyme. Eur. J. Biochem 204, 293-297.
25. Meggio, F., Boldyreff, B., Issinger, O.-G. & Pinna, L. A. (1994) Casein kinase 2 down-regulation and activation by polybasic peptides are mediated by acidic residues in the 55-64 region of the β-subunit. A study with calmodulin as phophorylatable substrate. Biochemistry 33, 4336-4342.
26. Pinna, L. A. (1990) Casein kinase 2: an 'eminence grise' in cellular regulation ? Biochim. Biophys. Acta 1054, 267-284.
27. Pyerin, W. (1994) Human casein kinase II: structures, genes, expression and requirement in cell growth stimulation. Adv. Enzyme Regul. 34, 225-246.
28. Pyerin, W., Ackermann, A. & Lorenz, P. (1996) in Protein phosphorylation (Marks, F., ed.) pp. 117-174, Verlag Chemie, Weinheim.
29. Sarno, S., Marin, O., Meggio, F. & Pinna, L. A. (1993) Polyamines as negative regulators of casein kinase-2. Biochem. Biophys. Res. Commun. 194, 83-90.
30. Tuazon, P. T. & Traugh, J. A. (1991) Casein kinase I and II - multipotential serine protein kinases: structure, function, and regulation. Adv. Second Messenger Phosphoprotein Res. 23, 123-164.