A flash-type bioluminescent immunoassay that is more sensitive than radioimaging: Quantitative detection of cytokine cDNA in activated and resting human cells

Journal of Immunological Methods

Volumn 211, Issue 1-2, 1998, Pages 65-77

  Actor, J K ^a   Kuffner, T ^b,c   Dezzutti, C S ^c   Hunter, R L ^a   McNicholl, J M ^b,c  

^a UNIVERSITY OF TEXAS   (United States)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c Immunology Branch   (United States)

Author keywords

Aequorin; Bioluminescence; Cytokine; mRNA; Quantitative RT PCR; Radioimaging

Indexed keywords

CD3 ANTIGEN; COMPLEMENTARY DNA; CYTOKINE; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; INTERLEUKIN 10; INTERLEUKIN 12; INTERLEUKIN 1ALPHA; INTERLEUKIN 1BETA; INTERLEUKIN 2; INTERLEUKIN 4; INTERLEUKIN 5; INTERLEUKIN 6; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; BIOLUMINESCENCE; CONTROLLED STUDY; DNA DETERMINATION; HUMAN; HUMAN CELL; IMMUNOASSAY; LYMPHOCYTE ACTIVATION; NORMAL HUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

AEQUORIN; CYTOKINES; DNA, COMPLEMENTARY; HUMANS; IMMUNOASSAY; INDICATORS AND REAGENTS; LEUKOCYTES, MONONUCLEAR; LUMINESCENT MEASUREMENTS; MITOGENS; PHYTOHEMAGGLUTININS; POLYMERASE CHAIN REACTION; RADIOIMMUNOASSAY; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY;

EID: 0032004236     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(97)00190-7     Document Type: Article

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