CO2-sensitive neurons in organotypic cultures of the fetal rat medulla

Respiration Physiology

Volumn 111, Issue 2, 1998, Pages

  Wellner Kienitz, M C ^a   Shams, H ^a  

^a RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

Chemosensitivity, central, organotypic culture; Mammals, rat; Neurons, brainstem, central chemosensitivity

Indexed keywords

CARBON DIOXIDE;

ANIMAL TISSUE; ARTICLE; BRAIN SLICE; BRAIN STEM; BREATHING; CHEMOSENSITIVITY; ELECTROPHYSIOLOGY; FETUS; MEDULLA OBLONGATA; MEMBRANE POTENTIAL; NONHUMAN; POTASSIUM CURRENT; PRIORITY JOURNAL; RAT; SYNAPTIC TRANSMISSION; TISSUE CULTURE; ACTION POTENTIAL; ANIMAL; BREATHING MECHANICS; CULTURE TECHNIQUE; CYTOLOGY; DRUG EFFECT; DRUG RESISTANCE; FEMALE; NERVE CELL; PATCH CLAMP; PHYSIOLOGY; PREGNANCY; SPRAGUE DAWLEY RAT;

ACTION POTENTIALS; ANIMALS; CARBON DIOXIDE; CULTURE TECHNIQUES; DRUG RESISTANCE; ELECTROPHYSIOLOGY; FEMALE; FETUS; MEDULLA OBLONGATA; NEURONS; PATCH-CLAMP TECHNIQUES; PREGNANCY; RATS; RATS, SPRAGUE-DAWLEY; RESPIRATORY MECHANICS; SYNAPTIC TRANSMISSION;

EID: 0031994694     PISSN: 00345687     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0034-5687(97)00124-2     Document Type: Article

References (25)

1. Bingmann D., Baker R.E., Ballantyne D. Rhythm generation in brainstem cultures grown in a serum-free medium. Neurosci. Lett. 132:1991;167-170.
2. 2 decreases membrane conductance and depolarizes neurons in the nucleus tractus solitarii. Exp. Brain Res. 76:1989;656-661.
3. Dean J.B., Bayliss D.A., Erickson J.T., Lawing W.L., Millhorn D.E. Depolarization and stimulation of neurons in nucleus tractus solitarii by carbon dioxide does not require chemical synaptic input. Neurosci. 36:1990;207-216.
4. Di Pasquale E., Monteau R., Hillaire G. In vitro study of central respiratory like actvity of the fetal rat. Exp. Brain Res. 89:1992;459-464.
5. Fitzgerald S.C., Willis M.A., Yu C., Rigatto H. In search of central respiratory neurons: I. Dissociated cell cultures of respiratory areas from the uppper medulla. J. Neurosci. Res. 33:1992;579-589.
6. CO2. J. Appl. Physiol. 72:1992;439-446.
7. Kang Y., Kitai S.T. Calcium spike underlying rhythmic firing in dopaminergic neurons of the rat substantia nigra. Neurosci. Res. 18:1993a;195-207.
8. Kang Y., Kitai S.T. A whole-cell patch clamp study on the pacemaker potential in dopaminergic neurons of rat substantia nigra compacta. Neurosci. Res. 18:1993b;209-221.
9. Kangrga I.M., Loewy A.D. Whole-cell recordings from visualized C1 adrenergic bulbospinal neurons: ionic mechanisms underlying vasomotor tone. Brain Res. 670:1995;215-232.
10. Kawai A., Ballantyne D., Mückenhoff K., Scheid P. Chemosensitive medullary neurons in the brain-stem-spinal cord preparation of the neonatal rat. J. Physiol. London. 492:1996;277-292.
11. Loeschcke H.H. Central chemosensitivity and the reaction theory. J. Physiol. London. 332:1982;1-24.
12. Millhorn D.E., Eldridge F.L. Role of ventrolateral medulla in regulation of respiratory and cardiovascular systems. J. Appl. Physiol. 61:1986;1249-1263.
13. Neubauer J.A., Gonsalves S.F., Chou W., Geller H.M., Edelman N.H. Chemosensitivity of medullary neurons in explant tissue cultures. Neuroscience. 45:1991;701-708.
14. Onimaru H., Arata A., Homma I. Localization of respiratory rhythm-generating neurons in the medulla of brainstem-spinal cord preparations from newborn rats. Neurosci. Lett. 78:1987;151-155.
15. Onimaru H., Arata A., Homma I. Primary respiratory rhythm generator in the medulla of brainstem-spinal cord preparation from newborn rat. Brain Res. 445:1988;314-324.
16. Onimaru H., Arata A., Homma I. Firing properties of respiratory rhythm generating neurons in the absence of synaptic transmission in rat medulla in vitro. Exp. Brain Res. 76:1989;530-536.
17. Onimaru H., Homma I. Whole-cell recordings from respiratory neurons in the medulla of brainstem-spinal cord preparations isolated from newborn rats. Pflügers Arch. 420:1992;399-406.
18. Peers C., Buckler K.J. Transduction of chemostimuli by the type I carotid body cell. J. Membr. Biol. 144:1995;1-9.
19. 2 of neurons in the rostral ventral medulla in vitro. J. Neurophysiol. 73(3):1995;933-944.
20. 2 and low pH. J. Neurosci. Res. 33:1992;590-597.
21. 2 and low pH. Biol. Neonate. 149:1994;149-155.
22. Schlaefke M.E. Central chemosensitivity: a respiratory drive. Rev. Physiol. Biochem. Pharmacol. 90:1981;171-244.
23. Smith J.C., Ballanyi K., Richter D.W. Whole-cell patch clamp recordings from respiratory neurons in neonatal rat brain-stem in vitro. Neurosci. Lett. 134:1992;153-156.
24. Southard, T.L., Huang, R.Q., Dean, J.B., 1995. Electrophysiological properties of neurons in chemosensitive areas of the dorsal and ventral brain-stem. Proceedings from the 25th Annual Meeting of Society of Neuroscience. San Diego, California. 21 (3), 738.12.
25. Streit J., Spenger C., Lüscher H. An organotypic spinal cord-dorsal root ganglion-skeletal muscle coculture of embryonic rat. II. Functional evidence for the formation of spinal reflex arcs in vitro. Eur. J. Neurosci. 3:1991;1054-1068.