Use of flow cytometry as a tool to study mitochondrial membrane potential in isolated, living hepatocytes

Biochemistry (Moscow)

Volumn 63, Issue 2, 1998, Pages 235-238

  Salvioli, S ^a   Maseroli, R ^a   Pazienza, T L ^a   Bobyleva, V ^a   Cossarizza, A ^a  

^a UNIVERSITY OF MODENA AND REGGIO EMILIA   (Italy)

Author keywords

6 ketocholestanol; Cytofluorimetry; FCCP; Hepatocytes; Mitochondrial membrane potential

Indexed keywords

ANALYTIC EQUIPMENT; BIOCHEMISTRY; CELL MEMBRANES; FLUORESCENCE; FLUOROMETERS;

CYTOFLUORIMETRY; FLOW CYTOMETRY; MITOCHONDRIAL MEMBRANE;

CYTOLOGY;

5,5',6,6' TETRACHLORO 1,1',3,3' TETRAETHYLBENZIMIDAZOLOCARBOCYANINE; 5,5',6,6'-TETRACHLORO-1,1',3,3'-TETRAETHYLBENZIMIDAZOLOCARBOCYANINE; 6 OXOCHOLESTANOL; 6-KETOCHOLESTANOL; BENZIMIDAZOLE DERIVATIVE; CARBOCYANINE; CARBONYL CYANIDE 4 (TRIFLUOROMETHOXY)PHENYLHYDRAZONE; CHOLESTEROL DERIVATIVE; FLUORESCENT DYE; UNCOUPLING PROTEIN;

ANIMAL; ARTICLE; CELL MEMBRANE POTENTIAL; CYTOLOGY; DRUG EFFECT; FLOW CYTOMETRY; IN VITRO STUDY; INTRACELLULAR MEMBRANE; LIVER; MALE; METABOLISM; METHODOLOGY; MITOCHONDRION; OXYGEN CONSUMPTION; PHYSIOLOGY; RAT; SPRAGUE DAWLEY RAT;

ANIMALS; BENZIMIDAZOLES; CARBOCYANINES; CARBONYL CYANIDE P-TRIFLUOROMETHOXYPHENYLHYDRAZONE; FLOW CYTOMETRY; FLUORESCENT DYES; INTRACELLULAR MEMBRANES; KETOCHOLESTEROLS; LIVER; MALE; MEMBRANE POTENTIALS; MITOCHONDRIA; OXYGEN CONSUMPTION; RATS; RATS, SPRAGUE-DAWLEY; UNCOUPLING AGENTS;

EID: 0031992916     PISSN: 00062979     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (15)

1. Nobes, C. D., Brown, G. C., Olive, P. N., and Brand, M. D. (1990) J. Biol. Chem., 265, 12903-12909.
2. Harper, M.-E., and Brand, M. (1993) J. Biol. Chem., 268, 14850-14860.
3. Cossarizza, A., Baccarani Contri, M., Kalashnikova, G., and Franceschi, C. (1993) Biochem. Biophys. Res. Commun., 197, 40-45.
4. Cossarizza, A., Ceccarelli, D., and Masini, A. (1996) Exp. Cell. Res., 222, 84-94.
5. Hada, H., Honda, C., and Tanemura, H. (1997) Photogr. Sci. Eng., 21, 83-91.
6. Reers, M., Smith, T. W., and Chen, L. B. (1991) Biochemistry, 30, 4480-4486.
7. Smiley, S. T., Reers, M., Mottola-Hartshorn, C., Lin, M. M., Chen, A., Smith, T. W., Steel, G. D., and Chen, L. B. (1991) Proc. Natl. Acad. Sci. USA, 88, 3671-3675.
8. Starkov, A. A., Bloch, D. A., Chernyak, B. V., Dedukhova, V. I., Mansurova, S. E., Severina, I. I., Simonyan, R. A., Vygodina, T. V., and Skulachev, V. P. (1997) Biochim. Biophys. Acta, 1318, 159-172.
9. Starkov, A. A., Dedukhova, V. I., and Skulachev, V. P. (1994) FEBS Lett., 355, 305-308.
10. Starkov, A. A., Simonyan, R. A., Dedukhova, V. I., Mansurova, S. E., Palamarchuk, L. A., and Skulachev, V. P. (1997) Biochim. Biophys. Acta, 1318, 159-172.
11. Krebs, H. A. (1974) Regulation of Hepatic Metabolism (Lundquist, F., and Tystrup, N., eds.) Academic Press, New York, pp. 726-750.
12. Cossarizza, A., Franceschi, C., Monti, D., Salvioli, S., Bellesia, E., Rivabene, R., Biondo, L. G. R., Tinari, A., and Malomi, W. (1995) Exp. Cell Res., 220, 232-240.
13. Polla, B. S., Kantengwa, S., Francois, D., Salvioli, S., Franceschi, C., Marsac, C., and Cossarizza, A. (1996) Proc. Natl. Acad. Sci. USA, 93, 6458-6463.
14. Bobyleva, V., Bellei, M., Pazienza, T. L., and Muscatello, U. (1997) Biochem. Mol. Biol. Int., 41, 469-480.
15. Layne, E. (1957) Meth. Enzymol., 3, 447-454.