U6atac snRNA, the highly divergent counterpart of U6 snRNA, is the specific target that mediates inhibition of AT-AC splicing by the influenza virus NS1 protein

RNA

Volumn 4, Issue 1, 1998, Pages 55-64

  Wang, Weirong ^a   Krug, Robert M ^a  

^a RUTGERS UNIVERSITY   (United States)

Author keywords

AT AC splicing; Influenza virus NS1 protein; RNA binding protein; U6 snRNA; U6atac snRNA

Indexed keywords

MESSENGER RNA PRECURSOR; RNA BINDING PROTEIN; SMALL NUCLEAR RNA;

ALTERNATIVE RNA SPLICING; ARTICLE; BINDING SITE; CHEMICAL MODIFICATION; CROSS LINKING; INFLUENZA VIRUS; INTRON; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; PROTEIN BINDING; RNA BINDING; RNA SPLICING; SPLICEOSOME;

BASE SEQUENCE; BINDING SITES; HUMANS; MOLECULAR SEQUENCE DATA; RNA SPLICING; RNA, SMALL NUCLEAR; SPLICEOSOMES; VIRAL NONSTRUCTURAL PROTEINS;

INFLUENZA VIRUS; MAMMALIA;

EID: 0031985445     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Alonso-Caplen FV, Nemeroff ME, Qiu Y, Krug RM. 1992. Nucleo-cytoplasmic transport: The influenza virus NS1 protein regulates the transport of spliced NS2 mRNA and its precursor NS1 mRNA. Genes & Dev 6:255-267.
2. Brow DA, Guthrie C. 1988. Spliceosomal RNA U6 is remarkably conserved from yeast to mammals. Nature 334:213-218.
3. Chien C, Tejero R, Huang Y, Zimmerman DE, Rios CB, Krug RM, Montelione GT. 1997. A novel RNA-binding motif in influenza A virus non-structural protein 1. Nature Struct Biol. Forthcoming.
4. Fortes P, Beloso A, Ortin J. 1994. Influenza virus NS1 protein inhibits pre-mRNA splicing and blocks mRNA nucleocytoplasmic transport. EMBO J 13:704-712.
5. Gontarek RR, McNally MT, Beemon K. 1993. Mutation of an RSV intronic element abolishes both U11/U12 snRNP binding and negative regulation of splicing. Genes & Dev 7:1926-1936.
6. Hall SL, Padgett RA. 1994. Conserved sequences in a class of rare eukaryotic nuclear introns with non-consensus splice sites. J Mol Biol 239:357-365.
7. Hall SL, Padgett RA. 1996. Requirement of U12 snRNA for in vivo splicing of a minor class of eukaryotic nuclear pre-mRNA introns. Science 271:1716-1718.
8. Hamm J, Kamaier M, Mattaj IW. 1987. In vitro assembly of U1 snRNPs. EMBO J 6:3479-3485.
9. Hatada E, Futada R. 1992. Binding of influenza A virus NS1 protein to dsRNA in vitro. J Gen Virol 73:3325-3329.
10. Jackson IJ. 1991. A reappraisal of non-consensus mRNA splice sites. Nucleic Acids Res 19:3795-3798.
11. Konarska MM. 1989. Analysis of splicing complexes and small nuclear ribonucleoprotein particles by native gel electrophoresis. Methods Enzymol 180:442-453.
12. Krug RM, Alonso-Caplen FV, Julkunen I, Katze MG. 1989. Expression and replication of the influenza virus genome. In: Krug RM, ed. The influenza viruses. New York: Plenum Press, pp 89-152.
13. Liu J, Lynch PA, Chien CY, Montelione GT, Krug RM, Berman HM. 1997. Crystal structure of the unique multifunctional RNA-binding domain of the influenza virus NS1 protein. Nature Struct Biol. Forthcoming.
14. Lu Y, Qian X, Krug RM. 1994. The influenza virus NS1 protein: A novel inhibitor of pre-mRNA splicing. Genes & Dev 8:1817-1828.
15. Lu Y, Wambach M, Katze MG, Krug RM. 1995. Binding of the influenza virus NS1 protein to double-stranded RNA inhibits the activation of the protein kinase that phosphorylates the eIF-2 translation initiation factor. Virology 214:222-228.
16. Madhani HD, Guthrie C. 1992. A novel base-paring interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome. Cell 71:803-817.
17. Nemeroff ME, Qian X, Krug RM. 1995. The influenza virus NS1 protein forms multimers in vitro and in vivo. Virology 212:422-428.
18. Nilsen TW. 1994. RNA-RNA interactions in the spliceosome: Unraveling the ties that bind. Cell 78:1-4.
19. Nilsen TW. 1996. A parallel spliceosome. Science 273:1813-1813.
20. Peattie D. 1979. Direct chemical method for sequencing RNA. Proc Natl Acad Sci USA 76:1760-1764.
21. Qian X, Alonso-Caplen F, Krug RM. 1994. Two functional domains of the influenza virus NS1 protein are required for regulation of nuclear export of mRNA. J Virol 68:2433-2441.
22. Qian X, Chien C, Lu Y, Montelione GT, Krug RM. 1995. An amino-terminal polypeptide fragment of the influenza virus NS1 protein possesses specific RNA binding activity and largely helical backbone structure. RNA 1:948-956.
23. Qiu Y, Krug RM. 1994. The influenza virus NS1 protein is a poly(A)-binding protein that inhibits nuclear export of mRNAs containing poly(A). J Virol 68:2425-2432.
24. Qiu Y, Nemeroff ME, Krug RM. 1995. The influenza virus NS1 protein binds to a specific region in human U6 snRNA and inhibits U6-U2 and U6-U4 snRNA interactions during splicing. RNA 1:304-316.
25. Sharp PA. 1994. Split genes and RNA splicing. Cell 77:805-815.
26. Shih S, Nemeroff ME, Krug RM. 1995. The choice of alternative 5′ splice sites in influenza virus M1 mRNA is regulated by the viral polymerase complex. Proc Natl Acad Sci USA 92:6324-6328.
27. Tarn W, Steitz JA. 1996a. A novel spliceosome containing U11, U12, and U5 snRNPs excises a minor class (AT-AC) intron in vitro. Cell 84:801-811.
28. Tarn WY, Steitz JA. 1996b. Highly diverged U4 and U6 small nuclear RNAs required for splicing rare AT-AC introns. Science 273:1824-1832.
29. Wang W, Krug RM. 1996. The RNA-binding and effector domains are conserved to different extents among influenza A and B viruses. Virology 223:41-50.
30. Wolff T, Bindereif A. 1993. Conformational changes of U6 during the spliceosome cycle: An intramolecular helix is essential both for initiating the U4-U6 interaction and for the first step in splicing. Genes & Dev 7:1377-1389.