Photoaffinity labeling by 4-thiodideoxyuridine triphosphate of the HIV- 1 reverse transcriptase active site during synthesis. Sequence of the unique labeled hexapeptide

Journal of Biological Chemistry

Volumn 273, Issue 2, 1998, Pages 997-1002

  Lin, Shanhua ^a,c   Henzel, William J ^b   Nayak, Sunil ^a,d   Dennis, Don ^a  

^a UNIVERSITY OF DELAWARE   (United States)

^b GENENTECH INC   (United States)

^c JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d JOHNS HOPKINS BLOOMBERG SCHOOL OF PUBLIC HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYURIDINE DERIVATIVE; RNA DIRECTED DNA POLYMERASE; VIRUS ENZYME;

ARTICLE; CONTROLLED STUDY; DNA SYNTHESIS; ENZYME ACTIVE SITE; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; PHOTOAFFINITY LABELING; PRIORITY JOURNAL; X RAY CRYSTALLOGRAPHY;

BINDING SITES; CRYSTALLOGRAPHY, X-RAY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HIV-1 REVERSE TRANSCRIPTASE; OLIGOPEPTIDES; PEPTIDE FRAGMENTS; PHOTOAFFINITY LABELS; THIONUCLEOTIDES; URIDINE TRIPHOSPHATE;

EID: 0031985229     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.273.2.997     Document Type: Article

References (31)

1. Majumdar, C., Abbotts, J., Broder, S., and Wilson, S. H. (1988) J. Biol. Chem. 263, 15657-15665
2. Huber, H. E., McCoy, J. M., Seehra, J. S., and Richardson, C. C. (1989) J. Biol. Chem. 264, 4669-4678
3. Huber, H. E., and Richardson, C. C. (1990) J. Biol. Chem. 265, 10565-10573
4. Kedar, P. S., Abbotts, J., Kovács, T., Lesiak, K., Torrence, P., and Wilson, S. H. (1990) Biochemistry 29, 3603-3611
5. Reardon, J. E., and Miller, W. H. (1990) J. Biol. Chem. 265, 20302-20307
6. Kati, W. M., Johnson, K. A., Jerva, L. F., and Anderson, K. S. (1992) J. Biol. Chem. 267, 25988-25997
7. Jaju, M., Beard, W. A., and Wilson, S. H. (1995) J. Biol. Chem. 270, 9740-9747
8. Larder, B. A., Purifoy, D. J., Powell, K. L., and Darby, G. (1987) Nature 327, 716-717
9. Larder, B. A., Kemp, S. D., and Purifoy, D. J. M. (1989) Proc. Natl. Acad. Sci. U. S. A. 86, 4803-4807
10. Basu, A., Tirumalai, R. S., and Modak, M. J. (1989) J. Biol. Chem. 264, 8746-8752
11. Mitchell, L. L. W., and Cooperman, B. S. (1992) Biochemistry 31, 7707-7713
12. Cheng, N., Merrill, B. M., Painter, G. R., Frick, L. W., and Furman, P. A. (1993) Biochemistry 32, 7630-7634
13. Basu, A., Ahluwalia, K. K., Basu, S., and Modak, M. J. (1992) Biochemistry 31, 616-623
14. Sheng, N., and Dennis, D. (1993) Biochemistry 32, 4938-4932
15. Rodgers, D. W., Gamblin, S. J., Harris, B. A., Ray, S., Culp, J. S., Helling, B., Woolf, D. J., Debouck, C., and Harrison, S. C. (1995) Proc. Natl. Acad. Sci. U. S. A. 90, 6320-6324
16. Hsiou, Y., Ding, J., Das, K., Clark, A. D., Jr., Hughes, S. H., and Arnold, E. (1996) Structure. 4, 853-860
17. Kohlstaedt, L. A., Wang, J., Friedman, J. M., Rice, P. A., and Steitz, T. A. (1992) Science 256, 1783-1790
18. Jacobo-Molina, J., Ding, J., Nanni, R. G., Clark, A. D., Jr., Lu, X., Tantillo, C., Williams, R. L., Kamer, G., Ferris, A. L., Clark, P., Hizi, A., Hughes, S. H., and Arnold, E. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 6320-6324
19. Patel, P. H., Jacobo-Molina, A., Ding, J., Tantilo, C., Clark, A. D., Jr., Raag, R., Nanni, R., G., Hughes, S. H., and Arnold, E. (1995) Biochemistry 34, 5351-5363
20. Steitz, T. A., Smerdon, S. J., Jäger, J., and Joyce, C. M. (1994) Science 266, 2022-2025
21. Ruth, J., and Cheng, K. (1981) Mol. Pharmacol. 20, 415-522
22. Laemmli, U. K. (1970) Nature 227, 680-685
23. Henzel, W. J., Rodriguez, H., and Watanabe, C. (1987) J. Chromatogr. 404, 41-52
24. Joyce, C. M., and Steitz, T. A. (1994) Annu. Rev. Biochem. 63, 777-822
25. Boyer, P. L., Ferris, A. L., and Hughes, S. H. (1992) J. Virol. 66, 1031-1039
26. Delarue, M., Poch, O., Tordo, N., Moras, D., and Argos, P. (1990) Protein Eng. 3, 461-467
27. Pelletier, H., Sawaya, M. R., Kumar, A., Wilson, S. H., and Kraut, J. (1994) Science 264, 1891-1903
28. Johnson, K. A. (1993) Annu. Rev. Biochem. 62, 685-713
29. Boyer, P. A., Tantillo, C., Jacobo-Molina, A., Nanni, R. G., Ding, J., Arnold, E., and Hughes, S. H. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 4882-4886
30. Sobol, R. W., Suhadolnik, R. J., Kumar, A., Lee, B. J., Hatfield, D. L., and Wilson, S. H. (1993) Biochemistry 30, 10623-10631
31. Lavrik, O. I., Prasad, R., Beard, W. A., Safronov, I. V., Dobrikov, M. I., Srivastava, D. K., Shishkin, G. V., Wood, T. G., and Wilson, S. H. (1996) J. Biol. Chem. 271, 21891-21897