Targeted gene deletion of Leishmania major genes encoding developmental stage-specific leishmanolysin (GP63)

Molecular Microbiology

Volumn 27, Issue 3, 1998, Pages 519-530

  Joshi, Phalgun B ^a,c   Sacks, David L ^b   Modi, Govind ^b   McMaster, W Robert ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^c Inex Pharmaceuticals   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE PROTEIN;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; GENE DELETION; IN VITRO STUDY; LEISHMANIA MAJOR; NONHUMAN; PARASITE DEVELOPMENT; PHLEBOTOMUS; PRIORITY JOURNAL; PROMASTIGOTE; SURVIVAL;

ANIMALS; BLOTTING, SOUTHERN; COMPLEMENT SYSTEM PROTEINS; FLOW CYTOMETRY; GENE DELETION; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENE TARGETING; GENES, PROTOZOAN; HOST-PARASITE RELATIONS; INSECT VECTORS; LEISHMANIA MAJOR; LEISHMANIASIS, CUTANEOUS; LIFE CYCLE STAGES; MACROPHAGES; METALLOENDOPEPTIDASES; MICE; MICE, INBRED BALB C; PHLEBOTOMUS; TRANSFECTION;

ANIMALIA; HAPTOPHYCEAE; LEISHMANIA MAJOR; MAMMALIA; PHLEBOTOMUS ARGENTIPES; PHLEBOTOMUS PAPATASI; PROTOZOA;

EID: 0031984118     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1998.00689.x     Document Type: Article

References (44)

1. Bouvier, J., Bordier, C., Vogel, H., Reichelt, R., and Etges, R. (1989) Characterization of the promastigote surface protease of Leishmania as a membrane-bound zinc endopeptidase. Mol Biochem Parasitol 37: 235-46.
2. Brittingham, A., Morrison, C.J., McMaster, W.R., McGwire, B.S., Chang, K.-P., and Mosser, D.M. (1995) Role of Leishmania surface protease GP63 in complement fixation, cell adhesion, and resistance to complement-mediated lysis. J Immunol 155: 3102-3111.
3. Button, L.L., and McMaster, W.R. (1988) Molecular cloning of the major surface antigen of Leishmania. J Exp Med 167: 724-729.
4. Button, L.L. Russell, D.G, Klein, H.L., Medina-Acosta, E., Karess, R.E., and McMaster, W.R. (1989) Genes encoding the major surface glycoprotein in Leishmania are tandemly linked at a single chromosomal locus and are constitutively transcribed. Mol Biochem Parasitol 32: 271-284.
5. Button, L.L., Reiner, N.E., and McMaster, W.R. (1991) Modification of GP63 genes from diverse species of Leishmania for expression of recombinant protein at high levels in Escherichia coli. Mol Biochem Parasitol 44: 213-224.
6. Button, L.L., Wilson, G., Astell, C.R., and McMaster, W.R. (1993) Recombinant Leishmania surface glycoprotein GP63 is secreted in the baculovirus expression system as a latent metalloproteinase. Gene 134: 75-81.
7. Chaudhuri, G., and Chang, K.-P. (1988) Acid protease activity of a major surface membrane glycoprotein (GP63) from Leishmania mexicana promastigotes. Mol Biochem Parasitol 27: 43-52.
8. Cooper, R., Ribeiro de Jesus, A., and Cross, G.A.M. (1993) Deletion of an immunodominant Trypanosoma cruzi surface glycoprotein disrupts flagellum-cell adhesion. J Cell Biol 122: 149-156.
9. Cruz, A, Coburn, C.M., and Beverley, S.M. (1991) Double targeted gene replacement for creating null mutant. Proc Natl Acad Sci USA 88: 7170-7174.
10. Etges, R., Bouvier, J., and Bordier, C. (1986) The major surface protein of Leishmania promastigotes is a protease. J Biol Chem 261: 9098-9101.
11. Etges, R. (1992) Identification of a surface metalloproteinase on 13 species of Leishmania isolated from humans, Crithidia fasciculata, and Herpetomonas samuelpessoai. Acta Tropica 50: 205-217.
12. Franke, E.D., McGreevy, P.B., Katz, S.P., and Sacks, D.L. (1985) Growth cycle-dependent generation of complement-resistant Leishmania promastigotes. J Immunol 134: 2713-2718.
13. Frommel, T.O., Button, L.L. Fujikura, Y. and. McMaster, W.R. (1990) The major surface glycoprotein (GP63) is present in both life stages of Leishmania. Mol Biochem Parasitol 38: 25-32.
14. Gritz, L., and Davies, J. (1983) Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene 25: 179-188.
15. Handman, E., and Goding, J.W. (1985) The Leishmania receptor for macrophages is a lipid-containing glycoconjugate. EMBO J 4: 329-336.
16. Heim, U., Tietze, E., Weschke, W., Tschape, H., and Wobus, U. (1989) Nucleotide sequence of a plasmid borne streptothricin-acetyl-transferase gene (sat-1). Nucleic Acids Res 17: 7103.
17. Inverso, J.A., Medina-Acosta, E., O'Conner, J., Russell, D.G., and Cross, G.A.M. (1993) Crithidia fasciculata contains a transcribed Leishmania surface proteinase (GP63) gene homologue. Mol Biochem Parasitol 57: 47-54.
18. Joshi, P.B., Webb, J.R., Davies, J.E., and McMaster, W.R. (1995) The gene encoding streptothricin acetyltransferase (sat) as a selectable marker for Leishmania expression vectors. Gene 156: 145-149.
19. Kapler, G.M., Coburn, C.M., and Beverley, S.M. (199?) Stable transfection of the human parasite Leishmania major delineates a 30-kilobase region sufficient for extra-chromosomal replication and expression. Mol Cell Biol 10: 1084-1094.
20. King, D.L., Chang, Y.D., and Turco, S.J. (1987) Cell surface lipophosphoglycan of Leishmania donovani. Mol Biochem Parasitol 24: 47-53.
21. McConville, M.J., Thomas-Oates, J.E., Ferguson, M.A., and Homans, S.W. (1990) Structure of the lipophosphoglycan from Leishmania major. J Biol Chem 265: 19611-19623.
22. Macdonald, M.H., Morrison, C.J, and McMaster., W.R. (1995) Analysis of the active site and activation mechanism of the Leishmania surface metalloproteinase GP63. Biochim Biophys Acta 1253: 199-207.
23. McGwire, B., and Chang., K-P. (1994) Genetic rescue of surface metalloproteinase (GP63)-deficiency in Leishmania amazonensis variants increases their infection of macrophages in the early phase. Mol Biochem Parasitol 66: 345-347.
24. McMaster, W.R., Morrison, C.J., Macdonald, M.H., and Joshi, P. (1994) Mutational and functional analysis of the Leishmania surface metalloproteinase GP63: similarities to matrix metalloproteinases. Parasitology 108: 29-36.
25. Medina-Acosta, E., and Cross, G.A.M. (1993) Rapid isolation of DNA from trypanosomatid protozoa using a simple 'mini-prep' procedure. Mol Biochem Parasitol 59: 327-329.
26. Medina-Acosta, E., Karess, R.E., Schwartz, H., and Russell, D.G. (1989) The promastigote surface protease (GP63) of Leishmania is expressed but differentially processed and localized in the amastigote stage. Mol Biochem Parasitol 37: 263-274.
27. Medina-Acosta, E., Karess, R.E., and Russell, D.G. (1993) Structurally distinct genes for the surface protease of Leishmania mexicana are developmentally regulated. Mol Biochem Parasitol 57: 31-46.
28. Pimenta, P.F.P., Turco, S.J., McConville, M.J., Lawyer, P.J. Perkins, P.V., and Sacks, D.L. (1992) Stage-specific adhesion of Leishmania promastigotes to the sandfly midgut. Science 256: 1812-1815.
29. Pimenta, P.F.P., Sariva, E.M.B., Rowton, E., Modi, G.B., Garraway, L.A., Beverley, S.M., et al. (1994) Evidence that the vectorial competence of phlebotomine sand flies for different species of Leishmania is controlled by structural polymorphisms in the surface lipophosphoglycan. Proc Natl Acad Sci USA 91: 9155-9159.
30. Puentes, S.M., Sacks, D.L., da Silva, R.P., and Joiner, K.A. (1988) Complement binding by two developmental stages of Leishmania major promastigotes varying in expression of a surface lipophosphoglycan. J Exp Med 167: 887-902.
31. Ratmmamoorthy, R., Donelson, J.E., Paetz, K.E., Maybodi, M., Roberts, S.C., and Wilson, M.E. (1992) Three distinct RNAs for the surface protease GP63 are differentially expressed during development of Leishmania donovani chagasi promastigotes to an infectious form. J Biol Chem 267: 1888-1895.
32. Russell, D.G. (1987) The macrophage-attachment glycoprotein GP63 is the predominant C3-acceptor site on Leishmania mexicana promastigotes. Eur J Biochem 165: 213-221.
33. Sacks, D.L, and da Silva, R.P. (1987) The generation of infective stage Leishmania major promastigotes is associated with the cell-surface expression and release of a developmentally regulated glycoprotein. J Immunol 139: 3099-3106.
34. Sacks, D.L., Brodin, T.N., and Turco, S.L. (1990) Developmental modification of the lipophosphoglycan from Leishmania major promastigotes during metacyclogenesis. Mol Biochem Parasitol 42: 225-234.
35. Sacks, D.L., Pimenta, P.F.P., McConville, M.J., Schneider, P., and Turco, S.J. (1995) Stage-specific binding of Leishmania donovani to the sand fly vector midgut is regulated by conformational changes in the abundant surface lipophosphoglycan. J Exp Med 181: 685-697.
36. Schlein, Y., and Romano, H. (1986) Leishmania major and L. donovani: effects on proteolytic enzymes of Phlebotomus papatasi. Exp Parasitol 62: 376-380.
37. Schlein, Y., Schnur, L.F., and Jacobson, R.L. (1990) Released glycoconjugate of indigenous Leishmania major enhances survival of a foreign L. major in Phlebotomus papatasi. Trans R Soc Trop Med Hyg 84: 353-355.
38. Souza, A.E., Bates, P.A. Coombs, G.H., and Mottram, J.C. (1994) Null mutants for the lmcpa cysteine proteinase gene in Leishmania mexicana. Mol Biochem Parasitol 63: 213-220.
39. Tietz, E., and Brevet, J. (1990) Nucleotide sequence of the streptothricin-acetyl-transferase gene sat2. Nucleic Acids Res 18: 1290.
40. Turco, S.J., and A. Descoteaux, A. (1992) The lipophosphoglycan of Leishmania parasites. Annu Rev Microbiol 46: 65-94.
41. Voth, B.R., Kelly, B.L., Joshi, P.B., Ivens, A.C., and McMaster, W.R. (1988) Differentially expressed Leishmania major gp63 genes encode cell surface leishmanolysin with distinct signals for glycosylphosphatidylinositol attachment. Mol Biochem Parasitol (in press).
42. Wallis, A.E, and McMaster, W.R. (1987) Identification of Leishmania genes encoding proteins containing tandemly repeating peptides. J Exp Med 166: 1814-1824.
43. Webb, J., and McMaster, W.R. (1994) Leishmania major HEXBP deletion mutants generated by double gene replacement. Mol Biochem Parasitol 63: 231-242.
44. Wiese, M., Ilg, T., Lottspeich, F., and Overath, P. (1995) Ser/ Thr-rich repetitive motifs as targets for phosphoglycan modifications in Leishmania mexicana secreted acid phosphatase. EMBO J 14: 1067-1074.