Identification of GCD14 and GCD15, novel genes required for translational repression of GCN4 mRNA in Saccharomyces cerevisiae

Genetics

Volumn 148, Issue 3, 1998, Pages 1007-1020

  Cuesta, Rafael ^a   Hinnebusch, Alan G ^b   Tamame, Mercedes ^a  

^a UNIVERSITY OF SALAMANCA   (Spain)

^b EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL RNA; MESSENGER RNA;

ARTICLE; FUNGAL GENETICS; GENE EXPRESSION; GENE MUTATION; GENETIC TRANSCRIPTION; GENOTYPE; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; RNA TRANSLATION; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION REGULATION; TRANSLATION REGULATION;

ALCOHOL OXIDOREDUCTASES; AMINOHYDROLASES; CLONING, MOLECULAR; DNA-BINDING PROTEINS; EPISTASIS, GENETIC; EUKARYOTIC INITIATION FACTOR-2; EUKARYOTIC INITIATION FACTOR-2B; FUNGAL PROTEINS; GENE DELETION; GENE EXPRESSION REGULATION, FUNGAL; GENES, DOMINANT; GENES, FUNGAL; GENES, RECESSIVE; GENES, REGULATOR; GENETIC COMPLEMENTATION TEST; MEIOSIS; MUTAGENESIS; PHENOTYPE; PROTEIN BIOSYNTHESIS; PROTEIN KINASES; PYROPHOSPHATASES; RNA, FUNGAL; RNA, MESSENGER; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

FUNGI; MYXOGASTRIA; SACCHAROMYCES CEREVISIAE;

EID: 0031982348     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (51)

1. ABASTADO, J. P., P. F. MILLER, B. M. JACKSON and A. G. HINNEBUSCH, 1991a Suppression of ribosomal reinitiation at upstream open reading frames in amino acid-starved cells forms the basis for GCN4 translational control Mol. Cell. Biol. 11: 486-496.
2. ABASTADO, J. P., P. F. MILLER and A. G. HINNEBUSCH, 1991b A quantitative model for translational control of the GCN4 gene of Saccharomyces cerevisiae. New Biol. 3: 511-524.
3. BOEKE, J. D., J. TRUEHEART, G. NATSOLIS and G. R. FINK, 1987 5-Fluoroorotic acid as a selective agent in yeast molecular genetics. Methods Enzymol. 154: 164-175.
4. BUSHMAN, J. L., A. I. ASURU, R. L. MATTS and A. G. HINNEBUSCH, 1993a Evidence that GCD6 and GCD7, translational regulators of GCN4, are subunits of the guanine nucleotide exchange factor for eIF-2 in Saccharomyces cerevisiae. Mol. Cell. Biol. 13: 1920-1932.
5. BUSHMAN, J. L., M. FOIANI, A. M. CIGAN, C. J. PADDON and A. G. HINNEBUSCH, 1993b Guanine nucleotide exchange factor for eukaryotic translation initiation factor 2 in Saccharomyces cerevisiae: interactions between the essential subunits GCD2, GCD6, and GCD7 and the regulatory subunit GCN3. Mol. Cell. Biol. 13: 4618-4631.
6. CIGAN, A. M., E. K. PABICH, L. FENG and T. F. DONAHUE, 1989 Yeast translation initiation suppressor sui2 encodes the α subunit of eukaryotic initiation factor 2 and shares sequence identity with the human α subunit. Proc. Natl. Acad. Sci. USA 86: 2784-2788.
7. CIGAN, A. M., M. FOIANI, E. M. HANNIG and A. G. HINNEBUSCH, 1991 Complex formation by positive and negative translational regulators of GCN4. Mol. Cell. Biol. 11: 3217-3228.
8. CIGAN, A. M., J. L. BUSHMAN, T. R. BOAL and A. G. HINNEBUSCH, 1993 A protein complex of translational regulators of GCN4 is the guanine nucleotide exchange factor for eIF-2 in yeast. Proc. Natl. Acad. Sci. USA 90: 5350-5354.
9. DEVER, T. E., L. FENG, R. C. WEK, A. M. CIGAN, T. D. DONAHUE et al., 1992 Phosphorylation of initiation factor 2α by protein kinase GCN2 mediates gene-specific translational control of GCN4 in yeast. Cell 68: 585-596.
10. DEVER, T. E., J. J. CHEN, G. N. BARBER, A. M. CIGAN, L. FENG et al., 1993 Mammalian eukaryotic initiation factor 2α kinases functionally substitute for GCN2 in the GCN4 translational control mechanism of yeast. Proc. Natl. Acad. Sci. USA 90: 4616-4620.
11. Met ternary complexes. Mol. Cell. Biol. 15: 6351-6363.
12. DONAHUE, T. F., and A. M. CIGAN, 1988 Genetic selection for mutations that reduce or abolish ribosomal recognition of the HIS4 translational initiatior region. Mol. Cell. Biol. 8: 2955-2963.
13. FOIANI, M., A. M. CIGAN, C. J. PADDON, S. HARASHIMA and A. G. HINNEBUSCH, 1991 GCD2, a translational repressor of the GCN4 gene, has a general function in the initiation of protein synthesis in Saccharomyces cerevisiae. Mol. Cell. Biol. 11: 3203-3216.
14. GARCÍA-BARRIO, M. T., T. NARANDA, C. R. VAZQUEZ DE ALDANA, R. CUESTA, A. G. HINNEBUSCH et al., 1995 GCD10, a translational repressor of GCN4, is the RNA-binding subunit of eukaryotic translation initiation factor-3. Genes Dev. 9: 1781-1796.
15. GRANT, C. M., and A. G. HINNEBUSCH, 1994 Effect of sequence context at stop codons on efficiency of reinitiation in GCN4 translational control. Mol. Cell. Biol. 14: 606-618.
16. GRANT, C. M., P. F. MILLER and A. G. HINNEBUSCH, 1995 Sequences 5′ of the first upstream open reading frame in GCA4 mRNA are required for efficient translational reinitiation. Nucleic Acids Res. 23: 3980-3888.
17. GUTHRIE, C., and G. R. FINK, 1991 Guide to yeast genetics and molecular biology. Methods Enzymol. 194.
18. HANNIG, E. M., and A. G. HINNEBUSCH, 1988 Molecular analysis of GCN3, a translational activator of GCN4: Evidence for posttranslational control of GCN3 regulatory function. Mol. Cell. Biol. 8: 4808-4820.
19. HANNIG, E. M., N. P. WILLIAMS, R. C. WEK and A. G. HINNEBUSCH, 1990 The translational activator GCN3 functions downstream from GCN1 and GCN2 in the regulatory pathway that couples GCN4 expression to amino acid availability in Saccharamyces cerevisiae. Genetics 126: 549-562.
20. HANNIG, E. M., A. M. CIGAN, B. A. FREEMAN and T. G. KINZY, 1993 GCD11, a negative regulator of GCN4 expression, encodes the gamma subunit of eIF-2 in Saccharomyces cerevisiae. Mol. Cell. Biol. 13: 506-520.
21. HARASHIMA, S., and A. G. HINNEBUSCH, 1986 Multiple GCD genes required for repression of GCN4, a transcriptional activator of the amino acid biosynthetic genes in Saccharommyces cerevisiae. Mol. Cell. Biol. 6: 3990-3998.
22. HARASHIMA, S., E. M. HANNIG and A. G. HINNEBUSCH, 1987 Interactions between positive and negative regulators of GCN4 controlling gene expression and entry into the yeast cell cycle. Genetics 117: 409-419.
23. HERSHEY, J. W. B., 1989 Protein phosphorylation controls translation rates. J. Biol. Chem. 264: 20823-20826.
24. HILL, D. E., and K. STRUHL, 1988 Molecular characterization of GCD1, a yeast gene required for general control of amino acid biosynthesis and cell-cycle initiation. Nucleic Acids Res. 16:9253-9265.
25. HINNEBUSCH, A. G., and G. R. FINK, 1983 Positive regulation in the general amino acid control of Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 80: 5374-5378.
26. HINNEBUSCH, A. G., 1985 A hierarchy of trans-acting factors modulates translation of an activator of amino acid biosynthetic genes in Saccharomyces cerevisiae. Mol. Cell. Biol. 5: 2349-2360.
27. HINNEBUSCH, A. G., 1988 Mechanisms of gene regulation in the general control of amino acid biosynthesis in Saccharomyces cerevisiae. Microbiol. Rev. 52: 248-273.
28. HINNEBUSCH, A. G., 1992 General and pathway-specific regulatory mechanisms controlling the synthesis of amino acid biosynthetic enzymes in Saccharomyces cerevisiae, pp. 319-414 in The Molecular and Cellular Biology of the Yeast Saccharomyces. Gene Expression, edited by E. W. JONES, J. R. PRINGLE and J. R. BROACH. Cold Spring Harbor Laboratory Press, Gold Spring Harbor, NY.
29. HINNEBUSCH, A. G., 1994a The eIF-2α kinases: Regulators of protein synthesis in starvation and stress. Cell. Biol. 5: 417-426.
30. HINNEBUSCH, A. G., 1994b Translational control of GCN4: An in vivo barometer of initiation-factor activity. Trends Biochem. Sci. 19: 409-414.
31. HINNEBUSCH, A. G., 1996 Translational control of GCN4: Gene-specific regulation by phosphorylation of eIF-2, pp. 199-244 in Translational Control, edited by J. W. B. HERSHEY, M. B. MATHEWS and N. SONENBERG. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
32. HINNEBUSCH, A. G., and S. W. LIEBMAN, 1991 Protein synthesis and translational control in Saccharomyces cerevisiae, pp. 627-735 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Genome Dynamics, Protein Synthesis, and Energetics, Vol. I, edited by J. R. BROACH, J. R. PRINGLE and E. W. JONES. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
33. ITO, H., Y. FUKADA, K. MURATA and A. KIMURA, 1983 Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153: 163-168.
34. JACKSON, R. J., 1991 Binding of met-RNA, pp. 193-223 in Translation in Eukaryotes, edited by H. TRACHSEL. CRC Press, Boca Raton, FL.
35. JOHNSTON, M., and R. W. DAVIS, 1984 Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. Mol. Cell. Biol. 4: 1440-1448.
36. LANKER, S., J. L. BUSHMAN, A. G. HINNEBUSCH, H. TRACHSEL and P. P. MÜELLER, 1992 Autoregulation of the yeast lysyl-tRNA-synthetase gene GCD5/KRS1 by translational and transcriptional control mechanisms. Cell 70: 647-657.
37. LUCCHINI, G., A. G. HINNEBUSCH, C. CHEN and G. R. FINK, 1984 Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae. Mol. Cell. Biol. 4: 1326-1333.
38. MARTON, M. J., D. CROUCH and A. G. HINNEBUSCH, 1993 GCN1, a translational activator of GCN4 in S. cerevisiae, is required for phosphorylation of eukaryotic translation initiation 2 by protein kinase GCN2. Mol. Cell. Biol. 13: 3541-3556.
39. MERRICK, W. C., 1992 Mechanism and regulation of eukaryotic protein synthesis. Microbiol. Rev. 56: 291-315.
40. MERRICK, W. C., and J. W. B. HERSHEY, 1996 The pathway and mechanism of eukaryotic protein synthesis, pp. 31-69 in Translational Control, edited by J. W. B. HERSHEY, M. B. MATHEWS and N. SONENBERG. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
41. MOEHLE, C. M., and A. G. HINNEBUSCH, 1991 Association of RAP1 binding sites with stringent control of ribosomal protein gene transcription in Saccharomyces cerevisiae. Mol. Cell. Biol. 11: 2723-2735.
42. MÜELLER, P. P., and A. G. HINNEBUSCH, 1986 Multiple upstream AUG codons mediate translational control of GCN4. Cell 45: 201-207.
43. PADDON, C. J., and A. G. HINNEBUSCH, 1989 gcd12 mutations are gcn3-dependent alleles of GCD2, a negative regulator of GCN4 in the general amino acid control of Saccharomyces cerevisiae. Genetics 122: 543-550.
44. ROLFES, R. J., and A. G. HINNEBUSCH, 1993 Translation of the yeast transcriptional activator GCN4 is stimulated by purine limitation: implications for the activation of the protein kinase GCN2. Mol. Cell. Biol. 13: 5099-5111.
45. SAMBROOK, J., E. F. FRITSCH and T. MANIATIS, 1989 Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
46. SIKORSKI, R. S., and P. HIETER, 1989 A system of shuttle vectors and yeast host strains designated for efficient manipulation of DNA in Saccharomyces cervisiae. Genetics 122: 19-27.
47. TZAMARIAS, D., I. Roussou and G. THIREOS, 1989 Coupling of GCN4 mRNA translationsl activation with decreased rates of polypeptide chain initiation. Cell 57: 947-954.
48. WEK, R. C., M. RAMIREZ, B. M. JACKSON and A. G. HINNEBUSCH, 1990 Identification of positive-acting domains in GCN2 protein kinase required for translational activation of GCN4 expression. Mol. Cell. Biol. 10: 2820-2831.
49. WILLIAMS, N. P., A. G. HINNEBUSCH and T. F. DONAHUF, 1989 Mutations in the structural genes for eukaryotic initiation factors 2α and 2β of Saccharomyces cerevisiae disrupt translational control of GCN4 mRNA. Proc.Natl. Acad. Sci. USA 86: 7515-7519.
50. WOLFNER, M., D. YEP, F. MESSENGUY and G. R. FINK, 1975 Integration of amino acid biosynthesis into the cell cycle of Saccharomyces cerevisiae. J. Mol. Biol. 96: 273-290.
51. YANG, W., and A. G. HINNEBUSCH, 1996 Identification of a regulatory subcomplex in the guanine nucleotide exchange factor eIF-2B that mediates inhibition by phosphorylated eIF-2. Mol. Cell. Biol. 16: 6603-6616.