Detergent and enzyme treatment of apoptotic cells for the observation of DNA fragmentation

BioTechniques

Volumn 24, Issue 4, 1998, Pages 558-560

  Park, Darren J ^a   Patek, Paul Q ^a  

^a UNIVERSITY OF HAWAII   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DETERGENT; DEXAMETHASONE; DNA FRAGMENT; PROTEINASE K; RECOMBINANT TUMOR NECROSIS FACTOR;

AGAR GEL ELECTROPHORESIS; ANIMAL CELL; APOPTOSIS; ARTICLE; CONTROLLED STUDY; MOUSE; NONHUMAN;

EID: 0031981841     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98244bm07     Document Type: Article

References (6)

1. .Fady, C., A. Gardner, F. Jacoby, K. Briskin, Y. Tu, I. Schmid and A. Lichtenstein. 1995. Atypical apoptotic cell death induced in L929 targets by exposure to tumor necrosis factor. J. Interferon Cytokine Res. 75:71-80.
2. Gera, J.F., C. Fady, A. Gardner, F.J. Jacoby, K.B. Briskin and A. Lichtenstein. 1993. Inhibition of DNA repair with aphidicolin enhances sensitivity of targets to tumor necrosis factor. J. Immunol. 757:3746-3757.
3. Jarvis, W.D., R.N. Kolesnick, F.A. Fornari, R.S. Traylor, D.A. Gewirtz and S. Grant. 1994. Induction of apoptotic DNA damage and cell death by activation of the sphingomyelin pathway. Proc. Natl. Acad. Sci. USA 91:73-77.
4. Kyprianou, N., R.B. Alexander and J.T. Isaacs. 1991. Activation of programmed cell death by recombinant human tumor necrosis factor plus topoisomerase II-targeted drugs in L929 tumor cells. J. Natl. Cancer Inst. 83:346-350.
5. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY.
6. Wyllie, A.H. 1980. Glucocorticoid-induced thymocytes apoptosis is associated with endogenous endonuclease activation. Nature 254:555-557.