Establishment of a stable steroidogenic porcine granulosa cell line

Journal of Molecular Endocrinology

Volumn 20, Issue 2, 1998, Pages 287-292

  Chedrese, P Jorge ^a   Rodway, Marie R ^a   Swan, Cynthia L ^a   Gillio Meina, Carolina ^a  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ANDROSTENEDIONE; ESTRADIOL; FOLLITROPIN; LUTEINIZING HORMONE; PROGESTERONE;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL IMMORTALIZATION; CONTROLLED STUDY; ESTROGEN SYNTHESIS; FEMALE; GRANULOSA CELL; NONHUMAN; PRIORITY JOURNAL; PROGESTERONE SYNTHESIS; PROTEIN CONTENT; PROTEIN DETERMINATION; STEROIDOGENESIS; SWINE;

ANIMALS; CELL DIVISION; CELL LINE, TRANSFORMED; CHOLERA TOXIN; CYCLIC AMP-DEPENDENT PROTEIN KINASES; ENZYME ACTIVATION; FEMALE; FOLLICLE STIMULATING HORMONE; FORSKOLIN; GRANULOSA CELLS; LUTEINIZING HORMONE; PROGESTERONE; SWINE;

EID: 0031981152     PISSN: 09525041     EISSN: None     Source Type: Journal    
DOI: 10.1677/jme.0.0200287     Document Type: Article

References (36)

1. Amsterdam, A., Zauberman, A., Meir, G., Pinhasi-Kimhi, O., and Suh, B. S. 1988. Cotransfection of granulosa cells with simian virus 40 and Ha-RAS oncogene generates stable lines capable of induced steroidogenesis. Proceedings of the National Academy of Sciences USA 85:7582-7586.
2. Amsterdam, A. and Selvaraj, N. 1997. Control of differentiation, transformation, and apoptosis in granulosa cells by oncogenes, oncoviruses, and tumor suppressor genes. Endocrine Reviews 18:435-461.
3. Bernath, V. A., Muro, A. F., Vitullo, A. D., Bley, M. A., Barañao, J. L. and Komblihtt, A. R. 1990. Cyclic AMP inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation. Journal of Biological Chemistry 265:18219-18226.
4. Butler, M. 1991. The characteristic and growth of culture cells. In Mammalian Cell Biotechnology A Practical Approach, pp. 1-24. Edited by M. Butler. IRL Press at Oxford University Press, New York, NY, USA.
5. Colman-Lerner, A. A., Salamone, D. F., Chiappe, M. E. and Barañao, J. L. 1995. Comparative studies between freshly isolated and spontaneously immortalized bovine granulosa cells: protein secretion, steroid metabolism, and responsiveness to growth factors. Journal of Cellular Physiology 164:395-403.
6. Channing, C. P. and Kammerman, S. 1973. Characteristics of gonadotropin receptors of porcine granulosa cells during follicle maturation. Endocrinology 92:531-540.
7. Channing, C. and Ledwitz-Rigby, F. 1975. Methods for assessing hormone-mediated differentiation of ovarian cells in culture and in short-term incubations. Methods of Enzymology 39:183-230.
8. Chedrese, P., Rajkumar, K., Ly, H. and Murphy, B. D. 1988. Dose response of luteinized porcine granulosa cells in vitro to prolactin: dependency on pre-exposure to human chorionic gonadotrophin. Canadian Journal of Physiology and Pharmacology 66:561-566.
9. Chedrese, P. J. Zhang, D. Luu The, V. Labrie, F. Juorio, A. and Murphy, B. 1990. Regulation of mRNA expression of 3β-hydroxy-5-ene steroid dehydrogenase in porcine granulosa cells in culture: A role for the protein kinase-C pathway. Molecular Endocrinology 4:1532-1538.
10. Chomczynski, P. and Sacchi, N. 1987. Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156-159.
11. Fainberg, A. P. and Vogelstein, B. 1983. A Technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 123:6-13.
12. Farkash, Y., Timberg, R. and Orly. J. 1986. Preparation of antiserum to rat cytochrome, and its use for ultrastructural localization of the immunoreactive enzyme by protein A-gold technique. Endocrinology 118:1253-1265.
13. Fitz, T. A., Wah, R. M., Schmidt, W. A. and Winkel, C. A. 1989. Physiologic characterization of transformed and cloned rat granulosa cells. Biology of Reproduction 40:250-258.
14. Freshney, R. I. 1994. Biology of the cultured cells. In Culture of Animal Cells, pp.9-16. Edited by R. I. Freshney. Wiley-Liss, New York, NY. USA.
15. Hinshelwood, M. M., Corbin, C. J., Tsang, P. C. W. and Simpson, E. R. 1993. Isolation and characterization of a complimentary deoxyribonucleic acid insert encoding bovine aromatase cytrocychrome P450. Endocrinology 133:1971-1993.
16. Hsueh, A. J. W., Adashi, E. Y., Jones, P. B. C. and Welsh, Jr. T. H. 1984. Hormonal Regulation of Differentiation of Cultured Ovarian Granulosa Cells. Endocrine Reviews 5:76-127.
17. Keren-Tal, I., Dantes, A., Sprengel, R. and Amsterdam, A. 1993. Establishment of steroidogenic granulosa cell lines expressing follicle stimulating hormone receptors. Molecular and Cellular Endocrinology 95:R1-R10.
18. Kwan, I., Farookhi, R., Huynh, H. T., Murphy, B. D., Turner, J. D and Downey, B. R. 1996. Steroidogenic Properties of a Spontaneously Established Porcine Granulosa Cell Line (PGC-2). Molecular Reproduction and Development 45:299-307.
19. Kwan, R. W. P., Lie, B-L., Auersperg, N., Thomas, E. E. and Leung, P. C. K. 1997. Biology of Reproduction, Vol. 56, Supplement 1, abstract 446.
20. Leighton, J. K., Grimes, R. W., Canning, S., and Hammond, J. M. 1993. Expression of the IGF system in primary and immortalized porcine ovarian granulosa cells. Molecular and Cell Endocrinology 97:29-35.
21. Luu The, V. L., Lachance, Y. Labrie, C., Thomas, J. L. and Labrie, F. 1989. Full length cDNA structure and deduced amino acid sequence of human 3β-hydroxy-5-ene steroid dehydrogenase. Molecular Endocrinology 3:1310-1312.
22. Lie, B-L., Leung, E., Leung, P. C. K. and Auersperg, N. 1996. Long-term growth and steroidogenic potential of human granulosalutein cells immortalized with SV40 large T antigen. Molecular and Cellular Endocrinology 120:169-176.
23. Lowsky, R. and Farookhi, R. 1989. A granulosa cell differentiation-stage-specific cytotoxic activity in bovine and rat sera. Biology of Reproduction 40:1265-1273.
24. Mulheron, G. W., Stone, R. T., Miller, W. L. and Wise, T. 1989. Nucleotide sequence of cytochrome P450 cholesterol side chain cleavage cDNA isolated from porcine testis. Nucleic Acid Research 174:1773.
25. Patton, A. C. and Collins, W. P. 1992. Differentiation processes of granulosa cells. Oxford Reviews of Reproductive Biology 14:169-221.
26. Rainey, W. H., Sawetawan, C., Shay, J. W., Michael, M. D., Mathis, J. M., Kutteh, W., Byrd, W. and Carr, B. R. 1994. Transformation of human granulosa cells with the E6 and E7 regions of human papillomavirus. Journal of Clinical Endocrinology and Metabolism 78:705-710.
27. Richards, J. S. 1994. Hormonal control of gene expression in the ovary. Endocrine Reviews 15:725-751.
28. Steel, R. G. D. and Torrie, J. H. 1980. Principles and procedures of statistics. A biometrical approach. McGraw-Hill, New York.
29. Suh, B-S. and Amsterdam, A. 1990. Establishment of highly steroidogenic granulosa cell lines by cotransfection with SV40 and Ha-ras oncogene: Induction of steroidogenesis by cyclic adenosine 3′-5′-monophosphate and its suppression by phorbol ester. Endocrinology 127:2489-2500.
30. Suh, B-S., Sprengel, R. Keren-Tal, I., Himmelhoch, S. and Amsterdam, A. 1992. Introduction of a gonadotropin receptor expression plasmid into immortalized granulosa cells leads to reconstitution of hormone-dependent steroidogenesis. The Journal of Cell Biology 119:439-450.
31. Tso, J. Y., Sun, X-H., Kao, T-H., Reece, K. S. and Wu, R. 1985. Isolation and characterization of rat and human gylceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene. Nucleic Acids Research 13:2485-2502.
32. Urban, R. J. and Veldhuis, J. D. 1992. Endocrine control of steroidogenesis in granulosa cells. Oxford Reviews of Reproductive Biology 14:225-262.
33. Xu, Y. P., Chedrese, P. J. and Thacker, P. A. 1997. Growth hormone as an amplifier of insulin-like growth factor-I action: potentiated oestradiol accumulation. Journal of Endocrinology 152:201-209.
34. Zeleznik, A. J., Hillier, S. G., Knazek, R. A., Ross, G. T. and Coon, H. G. 1979. Production of long term steroid-producing granulosa cell cultures by cell hybridization. Endocrinology 105:156-162.
35. Zlotkin, T., Farkash, Y. and Orly, J. 1986. Cell-specific expression of immunoreactive cholesterol side-chain cleavage cytochrome P-450 during follicular development in the rat ovary. Endocrinology 119:2809-2820.
36. Veldhuis, J. D., Klase, P. A. and Hammond, J. M. 1980. Divergent effects of prolactin upon steroidogenesis by porcine granulosa cells in vitro: influence of cytodifferentiation. Endocrinology 107:42-46.