Double labeling with fluorescence in situ hybridization in Drosophila whole-mount embryos

BioTechniques

Volumn 24, Issue 4, 1998, Pages 530-532

  Hughes, Sarah C ^a   Krause, Henry M ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

DIGOXIGENIN; ETHIDIUM BROMIDE; FLUORESCEIN; MESSENGER RNA; RNA;

ANIMAL TISSUE; ARTICLE; CHEMICAL LABELING; DNA TEMPLATE; DROSOPHILA; EMBRYO; FLUORESCENCE IN SITU HYBRIDIZATION; NONHUMAN; RNA PROBE;

DROSOPHILA;

EID: 0031980596     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98244bm01     Document Type: Article

References (6)

1. Goto, S. and S. Hayashi. 1997. Cell migration within the embryonic limb primordium of Drosophila as revealed by a novel fluorescence method to visualize mRNA and protein. Dev. Genes Evol. 207:194-198.
2. Hughes, S.C., B. Saulier-Le Drean, I. LivneBar and H.M. Krause. 1996. Fluorescence in situ hybridization in whole-mount Drosophila embryos. BioTechniques 20:748-750.
3. S.Lehmann, R. and D. Tautz. 1994. In situ hybridization to RNA, p. 575-598. In L.S.B. Goldstein and E.A. Fyrberg (Eds.), Methods in Cell Biology, Vol 44. Academic Press, NY.
4. Manoukian, A.S. and H.M. Krause. 1992. Concentration-dependent activities of the even-skipped protein in Drosophila embryos. Genes Dev. 6:1740-1751.
5. Patel, N.B., E. Martin-Blanco, K.G. Coleman, S.J. Poole, M.C. Ellis, T.B. Kornberg and C.S. Goodman. 1989. Expression of engrailed proteins in arthropods, annelids, and chordates. Cell 55:955-968.
6. Tautz, D. and C. Pfeiffle. 1989. A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback. Chromosoma 95:81-85.