Gonadotrophin-releasing hormone antagonist arrests premeiotic germ cell proliferation but does not inhibit meiosis in the male monkey: A quantitative analysis using 5-bromodeoxyuridine and dual parameter flow cytometry

Journal of Endocrinology

Volumn 156, Issue 1, 1998, Pages 23-34

  Weinbauer, G F ^a   Schubert, J ^a   Yeung, C H ^a   Rosiepen, G ^a   Nieschlag, E ^a  

^a INST OF REPRO MED OF THE UNIV   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

GONADOTROPIN;

ANIMAL EXPERIMENT; ARTICLE; CELL CYCLE S PHASE; CELL PROLIFERATION; FLOW CYTOMETRY; GROWTH REGULATION; HORMONAL REGULATION; HORMONE DEFICIENCY; MACACA; MALE; MEIOSIS; NONHUMAN; PRIORITY JOURNAL; SPERMATOGENESIS;

ANALYSIS OF VARIANCE; ANIMALS; BROMODEOXYURIDINE; CELL DIVISION; FLOW CYTOMETRY; GONADOTROPIN-RELEASING HORMONE; HORMONE ANTAGONISTS; INHIBINS; MACACA FASCICULARIS; MALE; MEIOSIS; ORGAN SIZE; RANDOM ALLOCATION; SPERMATOZOA; TESTIS; TESTOSTERONE;

EID: 0031975875     PISSN: 00220795     EISSN: None     Source Type: Journal    
DOI: 10.1677/joe.0.1560023     Document Type: Article

References (36)

1. Aravindan GR, Gopalakrishnan K, Ravindranath N & Moudgal NR 1993 Effect of altering endogenous gonadotrophin concentrations on the kinetics of testicular germ cell turnover in the bonnet monkey (Macaca radiata). Journal of Endocrinology 137 485-495.
2. Arslan MA, Weinbauer GF, Schlatt S, Shahab M & Nieschlag E 1993 FSH and testosterone, alone or in combination, initiate testicular growth and increase the number of spermatogonia and Sertoli cells in a juvenile non-human primate (Macaca mulatto). Journal of Endocrinology 136 235-243.
3. Chandolia RK, Weinbauer GF, Fingscheidt U, Bartlett JMS & Nieschlag E 1991a Effects of flutamide on testicular involution induced by an antagonist of gonadotrophin-releasing hormone and on stimulation of spermatogenesis by follicle-stimulating hormone in rat. Journal of Reproduction and Fertility 93 313-323.
4. Chandolia RK, Weinbauer GF, Simoni M, Behre HM & Nieschlag E 1991b Comparative effects of chronic administration of the non-steroidal antiandrogens flutamide and casodex on the reproductive system of the adult male rat. Acta Endocrinologica 125 547-555.
5. Clermont Y & Leblond CP 1959 Differentiation and renewal of spermatogonia in the monkey, Macacns rhesus. American Journal of Anatomy 104 237-273.
6. Clermont Y & Harvey SC 1965 Duration of the cycle of the seminiferous epithelium of normal, hypophysectomized and hypophysectomized-hormone treated albino rats. Endocrinology 76 80-89.
7. Dang DC 1971 Durée du cycle de l'épithelium séminifère du singe crabier, Macaca fascicularis. Annals de Biologie animale et Biochimie Biophysique 11 373-377.
8. Fingscheidt U, Weinbauer GF, Robertson DM, de Kretser DM & Nieschlag E 1989 Radioimmunoassay of inhibin in the serum of male monkeys. Journal of Endocrinology 122 477-483.
9. Fouquet JP & Dadoune JP 1986 Renewal of spermatogonia in the monkey (Macaca fascicularis). Biology of Reproduction 35 199-207.
10. Gopalkrishnan K, Kholkute S & Anand Kumar TC 1987 Estimation of daily sperm production in rat and monkeys. Journal of Biosciences 12 93-97.
11. Heller CG & Clermont Y 1964 Kinetics of the germinal epithelium in man. Recent Progress in Hormone Research 20 545-575.
12. Hoshino T, Nagashima T, Murovic J, Levin EM, Levin VA & Rupp SM 1985 Cell kinetic studies of in situ human brain tumors with bromodeoxyuridine. Cytometry 6 627-632.
13. Johnson L, Petty CS & Neaves WB 1980 A comparative study of daily sperm production and testicular composition in humans and rats. Bialagy of Reproduction 22 1233-1243.
14. Johnson L, Grumbles JS, Bagheri A & Petty CS 1990 Increased germ cell degeneration during postprophase of meiosis is related to increased serum follicle-stimulating hormone concentrations and reduced daily sperm production in aged men. Biology of Reproduction 42 281-287.
15. Johnson L, Chaturvedi PK & Williams JD 1992 Missing generations of spermatocytes and spermatids contribute to the low efficiency of spermatogenesis in humans. Biology of Reproduction 47 1091-1098.
16. van de Kant HJG & de Rooij DG 1992 Periodic acid incubation can replace hydrochloric acid hydrolysis and trypsin digestion in immunogold-silver staining of bromodeoxyuridine incorporation in plastic sections and allows the PAS reaction. Histochemical Journal 24 170-175.
17. McLachlan RI, Wreford NG, de Kretser DM & Robertson DM 1995 The effects of recombinant FSH on the restoration of spermatogenesis in the GnRH immunized adult rat. Endocrinology 136 4035-4043.
18. Marshall GR, Jockenhövel F, Lüdecke D & Nieschlag E 1986 Maintenance of complete but quantitatively reduced spermatogenesis in hypophysectomized monkeys by testosterone alone. Acta Endocrinologica 113 424-431.
19. O'Donnell L, McLachlan RI, Wreford BG, de Kretser DM & Robertson DM 1996 Testosterone withdrawal promotes stage-specific detachment of round spermatids from the rat seminiferous epithelium. Biology of Reproduction 55 895-901.
20. Rosiepen G, Weinbauer GF, Schlatt S, Behre HM & Nieschlag E 1994 Duration of the cycle of the seminiferous epithelium, estimated by the 5-bromodeoxyuridine technique, in laboratory and feral rats. Journal of Reproduction and Fertility 100 299-306.
21. Rosiepen G, Chapin RE & Weinbauer GF 1995 The duration of the cycle of the seminiferous epithelium is altered by administration of 2,5-hexanedione in the adult Sprague-Dawley rat. Journal of Andrology 16 127-135.
22. Rosiepen G, Arslan M, Nieschlag E & Weinbauer GF 1997 Estimation of the duration of the cycle of the seminiferous epithelium in the non-human primate Macaca mulatta using the 5-bromodeoxyuridine technique. Cell and Tissue Research 288 365-369.
23. Russell LD & Clermont Y 1977 Degeneration of germ cells in normal, hypophysectomized and hormone-treated hypophysectomized rats. Anatomical Record 187 347-366.
24. Russell LD & Petersen RN 1984 Determination of the elongated spermatid-Sertoli cell ratio in various mammals. Journal of Reproduction and Fertility 70 635-641.
25. Sharpe RM 1994 Regulation of spermatogenesis. In The Physiology of Reproduction, edn 2, pp 1363-1434. Eds E Knobil & JD Neill. New York: Raven Press.
26. Sinha-Hikim AP & Swerdloff RS 1993 Temporal and stage-specific changes in spermatogenesis of rat after gonadotrophin deprivation by a potent gonadotrophin-releasing hormone antagonist treatment. Endocrinology 133 2161-2170.
27. Sun YT, Wreford NG, Robertson DM & de Kretser DM 1990 Quantitative cytological studies of spermatogenesis in intact and hypophysectomized rats: identification of androgen-dependent stages. Endocrinology 127 1214-1223.
28. Thoolen B 1990 BrdUrd labelling of S-phase cells in testes and small intestine of mice, using microwave irradiation for immunogold-silver staining: an immunocytochemical study. Journal of Histochemistry and Cytochemistry 3 267-273.
29. Toppari J, Eerola E & Parvinen M 1985 Flow cytometric DNA analysis of defined stages of rat seminiferous epithelial cycle during in vitro differentiation, Journal of Andrology 6 325-333.
30. Weinbauer GF & Nieschlag E 1988 Reversibility of GnRH agonist-induced inhibition of testicular function: comparison between rat, monkey and man. In LH-RH Agonists in Oncology, pp 91-103. Ed K Höffken. Berlin: Springer-Verlag.
31. Weinbauer GF & Nieschlag E 1993 Comparison of the antigonadotropic activity of three GnRH antagonists (Nal-Glu, antide and cetrorelix) in a non-human primate model (Macaca fascicularis). Andrologia 25 141-147.
32. Weinbauer GF & Nieschlag E 1996 The Leydig cell as a target for male contraception. In The Leydig Cell, pp 629-662. Eds AH Payne, MP Hardy & LD Russell. Vienna: Cache River Press.
33. Weinbauer GF, Behre HM, Fingscheidt U & Nieschlag E 1991 Human follicle-stimulating hormone exerts a stimulatory effect on spermatogenesis, testicular size, and serum inhibin levels in the gonadotropin-releasing hormone antagonist-treated non-human primate (Macaca fascicularis). Endocrinology 129 1831-1839.
34. Weinbauer GF, Limberger A, Behre HM & Nieschlag E 1994 Can testosterone alone maintain the gonadotrophin-releasing hormone antagonist-induced suppression of spermatogenesis in the non-human primate? Journal of Endocrinology 142 485-495.
35. Wing TY & Christensen K 1982 Morphometric studies on rat seminiferous tubules. American Journal of Anatomy 165 13-25.
36. Zhengwei Y, Wreford N & de Kretser DM 1990 A quantitative study of spermatogenesis in the developing rat testis. Biology of Reproduction 43 629-635.