Immunocytochemical localization of the prohormone convertases PC1 and PC2 in rat prolactin cells

Journal of Histochemistry and Cytochemistry

Volumn 46, Issue 1, 1998, Pages 101-108

  Muller, Laurent ^a,c   Picart, Renée ^a   Barret, Alain ^a   Seidah, Nabil G ^b   Tougard, Claude ^a  

^a l'alimentation et l'Environnement   (France)

^b CLIN RESEARCH INSTITUTE OF MONTREAL   (Canada)

^c LOUISIANA STATE UNIV MEDICAL CENTER   (United States)

Author keywords

Electron microscope immunoperoxidase; Immunogold; Lactotropes; PC1; PC2; Prohormone convertases; Rat anterior pituitary

Indexed keywords

FURIN;

ANIMAL CELL; ARTICLE; CELLULAR DISTRIBUTION; GOLGI COMPLEX; HORMONE DETERMINATION; IMMUNOCYTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; NONHUMAN; PRIORITY JOURNAL; PROLACTIN SYNTHESIS; PROTEIN PROCESSING; RAT;

EID: 0031975344     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215549804600113     Document Type: Article

References (46)

1. Benjannet S, Rondeau N, Day R, Chretien M, Seidah NG (1991) PC1 and PC2 are proprotein convertases capable of cleaving proopiomelanocortin at distinct pairs of basic residues. Proc Natl Acad Sci USA 88:3564-3568
2. Benjannet S, Rondeau N, Paquet L, Boudreault A, Chretien M, Seidah NG (1993) Comparative biosynthesis, covalent post-translational modifications and efficiency of prosegment cleavage of prohormone convertases PC1 and PC2: glycosylation, sulphation and identification of the intracellular site of prosegment cleavage of PC1 and PC2. Biochem J 294:735-743
3. Benjannet S, Savaria D, Chretien M, Seidah NG (1995) 7B2 is a specific binding protein of the prohormone convertase PC2. J Neurochem 64:2303-2311
4. Braks JAM, Martens GJM (1994) 7B2 is a neuroendocrine chaperone that transiently interacts with prohormone convertase PC2 in the secretory pathway. Cell 78:263-273
5. Brinkley BR, Murphy P, Richardson LC (1967) Procedure for embedding in situ selected cells cultured in vitro. J Cell Biol 35:279-283
6. Day R, Schafer MKH, Watson SJ, Chretien M, Seidah NG (1992) Distribution and regulation of the prohormone convertases PC1 and PC2 in the rat pituitary. Mol Endocrinol 6:485-497
7. Dittie AS, Tooze SA (1995) Characterization of the endopeptidase PC2 activity towards secretogranin II in stably transfected PC12 cells. Biochem J 310:777-787
8. Duhau L, Grassi J, Grouselle D, Enjalbert A, Grognet JM (1991) An enzyme immunoassay for rat prolactin: application to the determination of plasma levels. J Immunoassay 12:233-250
9. Fisher-Colbrie R, Laslop A, Kirchmair R (1995) Secretogranin II: molecular properties, regulation of biosynthesis and processing to the neuropeptide secretoneurin. Prog Neurobiol 46:49-70
10. Friedman TC, Loh PY, Cawley NX, Birch NP, Huang SS, Jackson IMD, Nillni EA (1995) Processing of prothyrotropin-releasing hormone (pro-TRH) by bovine intermediate lobe secretory vesicle membrane PC1 and PC2 enzymes. Endocrinology 136:4462-4472
11. Guest PC, Arden SD, Bennet DL, Clark A, Rutherford NG, Hutton JC (1992) The post-translational processing and intracellular sorting of PC2 in the islets of Langerhans. J Biol Chem 267:22401-22406
12. Hoflehner J, Eder U, Laslop A, Seidah NG, Fisher-Colbrie R, Winkler HTI (1995) Processing of secretogranin II by prohormone convertases: importance of PCl in the generation of secretoneurin. FEBS Lett 360:294-298
13. Hornby PJ, Rosenthal SD, Mathis JP, Vindrola O, Lindberg I (1993) Immunocyto-chemical localization of the neuropeptide-synthesizing enzyme PC1 in AtT-20 cells. Neuroendocrinology 58:555-563
14. Huttner WB, Gerdes HH, Rosa P (1991a) Chromogranins/secretogranins - widespread constituents of the secretory granule matrix in endocrine cells and neurons. In Gratzl M, Langley K, eds. Markers for Neural and Endocrine Cells. Molecular Cell Biology, Diagnostic Applications. Weinheim, VCH, 93-131
15. Huttner WB, Gerdes HH, Rosa P (1991b) The granin (chromogranin/secretogranin) family. Trends Biochem Sci 16:27-30
16. Iacangelo AL, Eiden LE (1995) Chromogranin A current status as a precursor for bioactive peptides and a granulogenic/sorting factor in the regulated secretory pathway. Regul Pept 58:65-88
17. Kirchmair R, Hogue-Angeletti R, Guttierez J, Fisher-Colbrie R, Winkler H (1993) Secretoneurin - a neuropeptide generated in brain, adrenal medulla and other neuroendocrine tissues by proteolytic processing of secretogranin II (chromogranin C). Neuroscience 53:359-365
18. Lindberg I (1994) Evidence for the cleavage of the PC1/PC3 pro-segment in the endoplasmic reticulum. Mol Cell Neurosci 5:263-268
19. MacLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative. A new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077-1083
20. Malide D, Seidah NG, Chretien M, Bendayan M (1995) Electron microscopic immunocytochemical evidence for the involvement of the convertases PC1 and PC2 in the processing of proinsulin in pancreatic β-cells. J Histochem Cytochem 43:11-19
21. Marcinkiewicz M, Day R, Seidah NG, Chretien M (1993) Ontogeny of the prohormone convertases PC1 and PC2 in the mouse hypophysis and their colocalization with corticotropin and α-melanotropin. Proc Natl Acad Sci USA 90:4922-4926
22. Muller L, Barret A, Picart R, Tougard C (1997) Proteolytic processing of sulfated secretogranin II in the trans-Golgi network of GH3B6 cells. J Biol Chem 272:3669-3673
23. Muller L, Tougard C (1995) Production and secretion of N-terminal secretogranin II derived peptides in GH3B6 prolactin cells. Mol Cell Endocrinol 112:101-112
24. Natori S, Huttner WB (1994) Peptides derived from the granins (chromogranins/-secretogranins). Biochimie 76:277-282
25. Ozawa H, Picart R, Barret A, Tougard C (1994) Heterogeneity in the pattern of distribution of the specific hormonal product and secretogranins within the secretory granules of rat prolactin cells. J Histochem Cytochem 42:1097-1107
26. Picart R, Tixier-Vidal A (1974) Description d'une méthode permettant la sélection et l'étude ultrastructurale de plages cellulaires dans les monocouches hétérogènes cultivées en flacons de plastique. J Microsc 20:80a
27. Pu LP, Ma W, Barker JL, Loh YP (1996) Differential coexpression of genes encoding prothyrotropin-releasing hormone (pro-TRH) and prohormone convertases (PC1 and PC2) in rat brain neurons: implications for differential processing of pro-TRH. Endocrinology 137:1233-1241
28. Saria A, Troger J, Kirchmair R, Fisher-Colbrie R, Hogue-Angeletti R, Winkler H (1993) Secretoneurin releases dopamine from rat striatal slices: a biological effect of a peptide derived from secretogranin II (chromogranin C). Neuroscience 54:1-4
29. Seidah NG, Caspar L, Mion P, Marcinkiewicz M, Mbikay M, Chretien M (1990) cDNA sequence of two distinct pituitary proteins homologous to Kex2 and furin gene products: tissue-specific mR-NAs encoding candidates for prohormone processing proteinases. DNA Cell Biol 9:415-424
30. Seidah NG, Marcinkiewicz M, Benjannet S, Gaspar L, Beaubien G, Mattei MG, Lazure C, Mbikay M, Chretien M (1991) Cloning and primary sequence of a mouse candidate prohormone convertase PC1 homologous to PC2, furin and Kex2: distinct chromosomal localization and messenger RNA distribution in brain and pituitary compared to PC2. Mol Endocrinol 5:111-122
31. Shen FS, Seidah NG, Lindberg I (1993) Biosynthesis of the prohormone convertase PC2 in Chinese hamster ovary cells and in rat insulinoma cells. J Biol Chem 268:24910-24915
32. Sinha YN (1995) Structural variants of prolactin: occurrence and physiological significance. Endocrine Rev 16:354-369
33. Smeekens SP, Avruch AS, LaMendola J, Chan SJ, Steiner DF (1991) Identification of a cDNA encoding a second putative prohormone convertase related to PC2 in AtT-20 and islets of Langerhans. Proc Nad Acad Sci USA 88:340-344
34. Smeekens SP, Montag AG, Thomas G, Albiges-Rizo C, Carroll R, Benig M, Phillips LA, Martin S, Ohagi S, Gardner P, Swift HH, Steiner DF (1992) Proinsulin processing by the subtilisin-related proprotein convertases furin, PC2 and PC3. Proc Natl Acad Sci USA 89:8822-8826
35. Smeekens SP, Steiner DF (1990) Identification of a human insulinoma cDNA encoding a novel mammalian protein structurally related to the yeast dibasic processing protease Kex2. J Biol Chem 265:2997-3000
36. Thomas L, Leduc R, Thorne BA, Smeekens SP, Steiner DF (1991) Kex2-like endoproteases PC2 and PC3 accurately cleave a model prohormone in mammalian cells: evidence for a common core of neuroendocrine processing enzyme. Proc Natl Acad Sci USA 88:5297-5301
37. Tixier-Vidal A, Kerdelhué B, Jutisz M (1973) Kinetics of release of luteinizing hormone (LH) and follicle stimulating hormone (FSH) by primary cultures of dispersed rat anterior pituitary cells. Chronic effect of synthetic LH and FSH releasing hormone. Life Sci 12:499-509
38. Tougard C, Nasciutti LE, Picart R, Tixier-Vidal A, Huttner WB (1989) Subcellular distribution of secretogranins I and II in GH3 rat tumoral prolactin cells as revealed by electron microscopic immunocytochemistry. J Histochem Cytochem 37:1329-1336
39. Tougard C, Picart R (1986) Use of pre-embedding ultrastructural immunocytochemistry in the localization of a secretory product and membrane proteins in cultured prolactin cells. Am J Anat 175:161-177
40. Tougard C, Picart R, Tixier-Vidal A (1980) Electron microscopic cytochemical studies on the secretory process in rat prolactin cells in primary culture. Am J Anat 158:471-490
41. Tougard C, Tixier-Vidal A (1994) Lactotropes and Gonadotropes. In Knobil ED, Neill J, eds. The Physiology of Reproduction. 2nd ed. New York, Raven Press, 1711-1747
42. Vindrola O, Lindberg I (1992) Biosynthesis of the prohormone convertase PC1 in AtT-20 cells. Mol Endocrinol 6:1088-1094
43. Winkler H, Fisher-Colbrie R (1992) The chromogranins A and B: the first 25 years and future perspectives. Neuroscience 19:497-528
44. Zhou Y, Lindberg I (1993) Purification and characterization of the prohormone convertase PC1 (PC3). J Biol Chem 268:5615-5623
45. Zhu X, Lindberg I (1995) 7B2 facilitates the maturation of proPC2 in neuroendocrine cells and is required for the expression of enzymatic activity. J Cell Biol 129:1641-1650
46. Zhou A, Mains RE (1994) Endoproteolytic processing of proopiomelanocortin and prohormone convertases 1 and 2 in neuroendocrine cells overexpressing prohormone convertases 1 or 2. J Biol Chem 269:17440-17447