Autocrine/paracrine activator of cell proliferation: Purification of a 4-6 kD compound with growth-factor-like effects in Tetrahymena thermophila

Cellular Physiology and Biochemistry

Volumn 8, Issue 3, 1998, Pages 130-137

  Schousboe, Peter ^b   Wheatley, Denys N ^a   Rasmussen, Leif ^c  

^a UNIVERSITY OF ABERDEEN   (United Kingdom)

^b UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

^c NONE

Author keywords

Cell multiplication; Conditioned medium; Proliferation activating factor; Tetrahymena

Indexed keywords

GROWTH FACTOR;

ARTICLE; AUTOCRINE EFFECT; CELL DENSITY; CELL PROLIFERATION; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; CULTURE MEDIUM; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PURIFICATION; TETRAHYMENA THERMOPHILA; ULTRAFILTRATION;

EID: 0031972081     PISSN: 10158987     EISSN: None     Source Type: Journal    
DOI: 10.1159/000016276     Document Type: Article

References (20)

1. Christensen ST, Rasmussen L, Leick V, Wheatley DN: Signalling in unicellular eukaryotes. Regulation of cell survival, proliferation, differentiation, mating, chemosensory behaviour, and programmed cell death. Int Rev Cytol 1997;77:181-253.
2. Rasmussen L, Christensen ST, Schousboe P, Wheatley DN: Cell survival and multiplication. The overriding need for signals: From unicellular to multicellular systems. FEMS Microbiol Lett 1996;137: 123-128.
3. Christensen ST, Rasmussen L: Evidence for growth factors which control cell multiplication in Tetrahymena thermophila. Acta Protozool 1992;31:215-219.
4. Kidder GW: The effect of biologically conditioned medium on the growth rate and population yield of certain ciliated protozoa. Science 1939;90:405-406.
5. Szablewski L, Andreasen PH, Tiedtke A, Florin-Christensen J, Florin-Christensen M, Rasmussen L: Tetrahymena thermophila: Growth in synthetic nutrient medium in the presence and absence of glucose. J Protozool 1991;38:62-65.
6. Christensen ST, Wheatley DN, Rasmussen MI, Rasmussen L: Mechanisms controlling death, survival and proliferation in a model unicellular eukaryote cell system, Tetrahymena thermophila. Cell Death Differ 1995;2:301-308.
7. Laemmli UK, Favre MJ: Maturation of the head of bacteriophage T4.I. DNA packaging events. J Mol Biol 1973;80:575-599.
8. Merril CR, Goldman D, Ebert MH: Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 1981;211:1437-1438.
9. LeRoith D, Shiloach J, Roth J, Lesniak MA: Evolutionary origins of vertebrate hormones: Substances similar to mammalian insulins are native to unicellular eukaryotes. Proc Natl Acad Sci USA 1980;77: 6184-6188.
10. Christensen ST: Insulin rescues the unicellular eukaryote Tetrahymena from dying in a complete synthetic nutrient medium. Cell Biol Int 1993;17:833-837.
11. Christopher GK, Sundermann CA: Isolation and partial characterisation of the insulin binding sites of Tetrahymena pyriformis. Biochem Biophys Res Commun 1995;212: 515-523.
12. Wheatley DN, Christensen ST, Schousboe P, Rasmussen L: Cell signalling growth and death: Adequate nutrition alone may not be sufficient. Cell Biol Int 1993;17:817-823.
13. Kidder GW, Dewey VC: The biochemistry of ciliates in pure culture; in Lwoff A (ed): Biochemistry and Physiology of Protozoa. New York, Academic Press, 1951, pp 323-400.
14. Wildiers E: Nouvelle substance indispensable au développement de la levure. Cellule 1901;18:311-333.
15. Haberland G: Über Zellteilungshormone und ihre Beziehungen zur Wundheilung, Befruchtung, Parthenogenesis und Adventivembryonie. Biol Zentralbl 1922;42:145-171.
16. Fischer A: Contribution to the biology of tissue cells. I. The relation of cell crowding to tissue growth in vitro. J Exp Med 1923;38:667-673.
17. Robertson TB: The influence of washing upon the multiplication of isolated infusoria and upon the allelocatalytic effect in cultures initially containing two infusoria. Aust J Exp Biol Med Sci 1924;1:151-175.
18. Burrows MT: Factors regulating cellular growth and their importance in the explanation of cancer. South Med J 1924;17:233-243.
19. Kidder GW: Growth studies of ciliates. V. The acceleration and inhibition of ciliate growth in biologically conditioned medium. Physiol Zool 1941;14:209-226.
20. Tanabe H, Nishi M, Takagi Y, Wada F, Akamatsu I, Kaji K: Purification and identification of a growth factor produced by Paramecium tetraurelia. Biochem Biophys Res Commun 1990;170:786-792.