Potential of the immune complex transfer enzyme immunoassay for antigens and antibodies to improve the sensitivity and its limitations

Journal of Clinical Laboratory Analysis

Volumn 12, Issue 3, 1998, Pages 154-161

  Ishikawa, Eiji ^a   Ishikawa, Setsuko ^a   Hashida, Seiichi ^a   Hashinaka, Kazuya ^a  

^a UNIVERSITY OF MIYAZAKI   (Japan)

Author keywords

D galactosidase; Antibody IgG to HIV 1 p17 antigen; Antibody IgG to HIV 1 reverse transcriptase; Enzyme immuneassay; HIV 1 p24 antigen; Immune complex transfer

Indexed keywords

2,4 DINITROPHENOL; ANTIGEN P24; BETA GALACTOSIDASE; BIOTIN; HUMAN IMMUNODEFICIENCY VIRUS ANTIGEN; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN G ANTIBODY; RNA DIRECTED DNA POLYMERASE;

ANTIGEN ANTIBODY COMPLEX; ARTICLE; DIAGNOSTIC ACCURACY; ENZYME IMMUNOASSAY; SERUM;

ANTIBODIES; ANTIGEN-ANTIBODY COMPLEX; ANTIGENS; BETA-GALACTOSIDASE; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE PRODUCTS, GAG; HIV ANTIGENS; HIV CORE PROTEIN P24; HUMANS; IMMUNOASSAY; POLYSTYRENES; RNA-DIRECTED DNA POLYMERASE; VIRAL PROTEINS;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 0031970005     PISSN: 08878013     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2825(1998)12:3<154::AID-JCLA5>3.0.CO;2-9     Document Type: Article

References (18)

1. Kohno T, Ishikawa E: A novel enzyme immunoassay of anti-insulin IgG in guinea pig serum. Biochem Biophys Res Commun 147:644-649, 1987.
2. Ishikawa E, Kohno T: Development and applications of sensitive enzyme immunoassay for antibodies: a review. J Clin Lab Anal 3:252-265, 1989.
3. Ishikawa E, Hashida S, Tanaka K, Kohno T: Development and applications of ultrasensitive enzyme immunoassay for antigens and antibodies. Clin Chim Acta 185:223-230, 1989.
4. Ishikawa E, Hashida S, Kohno T, Hirota K: Ultrasensitive enzyme immunoassay. Clin Chim Acta 194:51-72, 1990.
5. Ishikawa E, Hashida S, Kohno T: Development of ultrasensitive enzyme immunoassay reviewed with emphasis on factors which limit the sensitivity. Mol Cell Probes 5:81-95, 1991.
6. Ishikawa E, Hashida S, Kohno T, Hirota K, Hashinaka K, Ishikawa S: Principle and applications of ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for antibodies in body fluids. J Clin Lab Anal 7:376-393, 1993.
7. Ishikawa E: Review: Immune complex transfer enzyme immunoassay for antibodies using recombinant proteins labeled with 2,4-dinitrophenyl group and β-D-galactosidase. Acta Histochem Cytochem 29:273-281, 1996.
8. Hashinaka K, Hashida S, Hirota K, et al.: Detection of antihuman immunodeficiency virus type 1 (HIV-1) immunoglobulin G in urine by an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) with recombinant reverse transcriptase as an antigen. J Clin Microbiol 32:819-822, 1994.
9. Hashida S, Hashinaka K, Nishikata I, et al.: Measurement of human immunodeficiency virus type 1 p24 in serum by an ultrasensitive enzyme immunoassay, the two-site immune complex transfer enzyme immunoassay. J Clin Microbiol 33:298-303, 1995.
10. Hashida S, Hashinaka K, Nishikata I, et al.: Ultrasensitive and more specific enzyme immunoassay (immune complex transfer enzyme immunoassay) for p24 antigen of HIV-1 in serum using affinity-purified rabbit anti-p24 Fab′ and monoclonal mouse anti-p24 Fab′. J Clin Lab Anal 10:302-307, 1996.
11. Hashida S, Hirota K, Hashinaka K, et al.: Detection of antibody IgG to HIV-1 in urine by sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant proteins as antigens for diagnosis of HIV-1 infection. J Clin Lab Anal 7:353-364, 1993.
12. Ishikawa S, Hashida S, Hashinaka K, et al.: Diagnosis of HIV-1 infection with whole saliva by detection of antibody IgG to HIV-1 with ultrasensitive enzyme immunoassay using recombinant reverse transcriptase as antigen. J Acquir Immune Defic Syndr Hum Retroviral 10:41-47, 1995.
13. Hashida S, Hashinaka K, Nishikata I, et al.: Immune complex transfer enzyme immunoassay that is more sensitive and specific than Western blotting for detection of antibody immunoglobulin G to human immunodeficiency virus type 1 in serum with recombinant pol and gag proteins as antigens. Clin Diagn Lab Immunol 2:535-541, 1995.
14. Ishikawa S, Hashida S, Hashinaka K, et al.: Whole saliva dried on filter paper for diagnosis of HIV-1 infection by detection of antibody IgG to HIV-1 with ultrasensitive enzyme immunoassay using recombinant reverse transcriptase as antigen. J Clin Lab Anal 10:35-41, 1996.
15. Hashida S, Hashinaka K, Nishikata I, et al.: Earlier diagnosis of HIV-1 infection by simultaneous detection of p24 antigen and antibody IgGs to p17 and reverse transcriptase in serum with enzyme immunoassay. J Clin Lab Anal 10:213-219, 1996.
16. Hashida S, Hashinaka K, Nishikata I, et al.: Shortening of the window period in diagnosis of HIV-1 infection by simultaneous detection of p24 antigen and antibody IgG to p17 and reverse transcriptase in serum with ultrasensitive enzyme immunoassay. J Virol Methods 62:43-53, 1996.
17. Hashida S, Ishikawa S, Nakamoto H,Tanaka S, Kojima M, IshikawaE: Simple and more sensitive immune complex transfer enzyme immunoassay for antibody IgG to reverse transcriptase of HIV-1 using microplates, modified polystyrene solid phase, and fluororeader. J Clin Lab Anal 10:294-301, 1996.
18. Ishikawa S, Hashida S, Hashinaka K, et al.: More sensitive immune complex transfer enzyme immunoassay for antibody IgG to p17 of HIV-1 with shorter incubation time for immunoreactions and larger volumes of serum samples. J Clin Lab Anal 11:244-250, 1997.