Effect of high concentrations of bile acids on cultured hepatocytes

Artificial Organs

Volumn 22, Issue 4, 1998, Pages 300-307

  Noto, Hiromitsu ^a   Matsushita, Michiaki ^a   Koike, Masahiko ^a   Takahashi, Manabu ^a   Matsue, Hirokazu ^a   Kimura, Jun ^a   Todo, Satoru ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

Author keywords

Bile acids; C CDC ratio; Hepatocellular injury; Rat hepatocyte

Indexed keywords

BODY FLUIDS; RATS;

ACID MIXTURES; BILE ACID; C/CDC RATIO; GLUCONEOGENESIS; HEPATOCELLULAR; HEPATOCELLULAR INJURY; HEPATOCYTES; LIVER-SPECIFIC FUNCTIONS; RAT HEPATOCYTES; TAUROCHOLATE;

AMINO ACIDS;

ALANINE AMINOTRANSFERASE; BILE ACID; DNA; GLUCOSE; GLYCOCHENODEOXYCHOLIC ACID; GLYCOCHOLIC ACID; TAUROCHENODEOXYCHOLIC ACID; TAUROCHOLIC ACID; UREA;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL STRUCTURE; CONTROLLED STUDY; DNA CONTENT; GLUCONEOGENESIS; LIVER CELL DAMAGE; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; UREA CYCLE;

ANIMALIA;

EID: 0031969138     PISSN: 0160564X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1525-1594.1998.05071.x     Document Type: Article

References (22)

1. Heuman DM, Pandak WM, Hylemon PB, Vlahcevic ZR. Conjugates of ursodeoxycholate protect against cytotoxicity of more hydrophobic bile salts: In vitro studies in rat hepatocytes and human erythrocytes. Hepatology Jpn 1991;14:920-6.
2. Shingai Y. Clinical significance of bile acid determination in patients with acute hepatic failure. Acta Hepatol Jpn 1988;29: 494-9 (in Japanese with an English abstract).
3. Kumada T, Nakano S, Ohta H, Sasaki T, Kitamura K, Watahiki H, Takeda I, Okuyama S, Takagi K. Clinical evaluation on determination of individual bile acids in liver disease. Acta Hepatol Jpn 1982;23:364-71 (in Japanese with an English abstract).
4. Ohiwa T, Katagiri K, Hoshino M, Hayakawa T, Nakai T. Tauroursodeoxycholate and tauro-β-muricholate exert cytoprotection by reducing intrahepatocyte taurochenodeoxycholate content. Hepatology 1993;17:470-6.
5. Endo S. Study on the cytotoxicity of bile acids on primary cultured rat hepatocytes. J Hukushima Med Col 1991;41: 65-78 (in Japanese with an English abstract).
6. Koga Y. Anti-cholestatic and cytoprotective properties of ursodeoxycholic acid-studies in vivo and vitro-. Acta Hepatol Jpn 1987;28:1597-604 (in Japanese with an English abstract).
7. Seglen PO. Preparation of rat liver cells. III. Enzymatic requirements for tissue dispersion. Exp Cell Res 1973;82:391-8.
8. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
9. Huggett AS, Nixon DA. Use of glucose oxidase, peroxidase and o-dianisidine in determination of blood and urinary glucose. Lancet 1957;2:368-70.
10. Marsh WH, Fingerhut B, Miller H. Automated and manual direct methods for the determination of blood urea. Clin Chem 1965;11:624-7.
11. Brunk CF, Jones KC, James TW. Assay for nanogram quantities of DNA in cellular homogenates. Anal Biochem 1979; 92:497-500.
12. The Committee on Enzymes of the Scandinavian Society for Clinical Chemistry and Clinical Physiology. Recommended methods for the determination of four enzymes in blood. Scand J Clin Lab Invest 1974;33:291-306.
13. Spivey JR, Bronk SF, Gores GJ. Glycochenodeoxycholate-induced lethal hepatocellular injury in rat hepatocytes. J Clin Invest 1993;92:17-24.
14. Gores GJ, Nieminen AL, Fleishman KE, Dawson TL, Herman B, Lemaslers JJ. Extracellular acidosis delays onset of cell death in ATP-depleted hepatocytes. Am J Physiol 1988; 255:C315-22.
15. Patel T, Bronk SF, Gores GJ. Increases of intracellular magnesium promote glycodeoxycholate-induced apoptosis in rat hepatocytes. J Clin Invest 1994;94:2183-92.
16. Zeid IM, Bronk SF, Fesmier PJ, Gores GJ. Cytoprotection by fructose and other ketohexoses during bile salt-induced apoptosis of hepatocytes. Hepatology 1997;25:81-6.
17. Kwo P, Patel T, Bronk SF, Gores GJ. Nuclear serine protease activity contributes to bile acid-induced apoptosis in hepatocytes. Am J Physiol 1995;268:G613-21.
18. Krähenbühl S, Talos C, Fischer S, Reichen J. Toxicity of bile acids on the electron transport chain of isolated rat liver mitochondria. Hepatology 1994;19:471-9.
19. Washizu T, Tomoda I, Kaneko JJ. Serum bile acid composition of the dog, cow, horse and human. J Vet Med Sci 1991; 53:81-6.
20. Azer SA, Kukongviriyapan V, Stacy NH. Mechanism of ketoconazole-induced elevation of individual serum bile acids in the rat: Relationship to the effect of ketoconazole on bile acid uptake by isolated hepatocytes. J Pharmacol Exp Ther 1995; 272:1231-7.
21. Kimura T, Shimamura M, Yamaguchi A, Katayama T, Kurita T, Tanaka A. Solubilization of cultured cell membrane by bile acids. Acta Hepatol Jpn 1981;22:1-7 (in Japanese with an English abstract).
22. Igimi H, Carey MC. pH-Solubility relations of chenodeoxycholic and ursodeoxycholic acids: Physical-chemical basis for dissimilar solution and membrane phenomena. J Lipid Res 1980;21:72-90.