Characterization of recombinant human plasma lecithin: Cholesterol acyltransferase (LCAT): N-linked carbohydrate structures and catalytic properties

Journal of Lipid Research

Volumn 39, Issue 4, 1998, Pages 807-820

  Lacko, Andras G ^a   Reason, Andrew J ^b,e   Nuckolls, Colin ^c   Kudchodkar, Bhalchandra J ^a   Nair, Maya P ^a   Sundarrajan, Geetha ^a   Pritchard, P Haydn ^d   Morris, Howard R ^b   Dell, Anne ^b  

^a UNIVERSITY OF NORTH TEXAS HEALTH SCIENCE CENTER   (United States)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

^c Och Spine at New York Presbyterian Hospitals   (United States)

^d UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^e M Scan Ltd   (United Kingdom)

Author keywords

Glycoprotein structure; LCAT

Indexed keywords

ASPARAGINE LINKED OLIGOSACCHARIDE; GLYCAN DERIVATIVE; GLYCOPEPTIDE; GLYCOPROTEIN; N ACETYLLACTOSAMINE SYNTHASE; PHOSPHATIDYLCHOLINE STEROL ACYLTRANSFERASE; PROLINE; RECOMBINANT ENZYME; TRYPSIN;

ANIMAL CELL; ARTICLE; CARBOHYDRATE ANALYSIS; CELL STRAIN BHK; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME STRUCTURE; FAST ATOM BOMBARDMENT MASS SPECTROMETRY; FUCOSYLATION; NONHUMAN; PRIORITY JOURNAL;

APOLIPOPROTEIN A-I; CARBOHYDRATE SEQUENCE; GLYCOPROTEINS; HUMANS; MOLECULAR SEQUENCE DATA; PHOSPHATIDYLCHOLINE-STEROL O-ACYLTRANSFERASE; PHOSPHOLIPASES; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS; SPECTROMETRY, MASS, FAST ATOM BOMBARDMENT;

EID: 0031968279     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (35)

1. Glomset, J. A. 1968. The plasma lecithin:cholesterol acyltransferase reaction. J. Lipid. Res. 9: 155-167.
2. Chung, J., D. A. Abano, G. M. Fless, and A. M. Scanu. 1979. Isolation, properties, and mechanism of in vitro action of lecithin:cholesterol acyltransferase from human plasma. J. Biol. Chem. 254: 7456-7464.
3. Chong, K. S., M. Jahani, S. Hara, and A. G. Lacko. 1983. Characterization of lecithin:cholesterol acyltransferase from human plasma III: Chemical properties of the enzyme. Can. J. Biochem. 61: 875-881.
4. McLean, J., C. J. Fielding, D. Drayna, H. Dieplinger, B. Baer, W. Kohr, W. Henzel, and R. Lawn. 1986. Cloning and expression of human lecithin:cholesterol acyltransferase cDNA. Proc. Natl. Acad. Sci. USA. 83: 2335-2339.
5. Yang, C. Y., D. Manoogian, Q. Pao, R. D. Knapp, A. M. Gotto, and H. J. Pownall. 1987. Lecithin:cholesterol acyltransferase. Functional regions and a structural model for the enzyme. J. Biol. Chem. 262: 3086-3091.
6. Miller, K. R., J. Wang, M. Sorci-Thomas, R. A. Anderson, and J. S. Parks. 1996. Glycosylation structures and enzyme activity of lecithin:cholesterol acyltransferase from human plasma. HepG2 cells and baculoviral and Chinese hamster ovary cell expression systems. J. Lipid. Res. 37: 551-561.
7. Schindler, P. A., C. A. Settineri, X. Collett, C. J. Fielding, and A. L. Burlingame. 1995. Site-specific detection and structural characterization of the glycosylation of human plasma lecithin:cholesterol acyltransferase and apolipoprotein D using HPLC/electrospray mass spectrometry and sequential glycosidase digestion. Proc. Sci. 4: 791-803.
8. O, K., J. S. Hill, X. Wang, R. McLeod, and P. H. Pritchard. 1993. Lecithin:cholesterol acyltransferase: role of N-linked glycosylation on the enzyme function. Biochem. J. 294: 879-884.
9. Francone, O., L. Evangelista, and C. J. Fielding. 1993. Lecithin-cholesterol acyltransferase: effects of mutagenesis at N-linked oligosaccharide attachment sites on acyl acceptor specificity. Biochim. Biophys. Acta. 1166: 301-304.
10. Hill, J., K. O, X. Wang, S. Paranjape, D. Dimitrijevich, A. G. Lacko, and P. H. Pritchard. 1993. Expression and characterization of recombinant human lecithin:cholesterol acyltransferase. J. Lipid. Res. 34: 1245-1251.
11. Chong, K. S., L. Davidson, R. Huttash, and A. G. Lacko. 1981. Characterization of lecithin:choleslerol acyltransferase from human plasma. Arch. Biochem. Biophys. 211: 119-124.
12. Skoza, I., and S. Mohos. 1976. Stable thiobarbituric acid chromophore with dimethyl sulphoxide. Application to sialic acid assay in analytical de-O-acetylation. Biochem. J. 159: 457-462.
13. Park, Y. B., and A. G. Lacko. 1986. Isolation and characterization of lecithin-cholesterol acyltransferase from hog plasma. Biochim. Biophys. Acta. 877: 179-190.
14. Batzri, S., and E. D. Korn. 1973. Single bilayer liposomes prepared without sonication. Biochim. Biophys. Acta. 298: 1015-1019.
15. Lacko, A. G., H. L. Rutenberg, and L. A. Soloff. 1972. Recovery of labeled cholesterol and cholesterol esters from thin-layer chromatograms. Clin. Chim. Acta. 39: 506-510.
16. Terpstra, A. H. M., C. J. H. Woodward, and F. J. Sanchez-Muniz. 1991. Improved technique for the separation of serum lipoproteins by density gradient ultracentrifugation: visualization by prestaining and rapid separation of serum lipoproteins from small volume of serum. Anal. Biochem. 111: 149-157.
17. Jahani, M., and A. G. Lacko. 1982. Study of the lecithin:cholesterol acyltransferase reaction with liposome and high density lipoprotein substrates. Biochim. Biophys. Acta. 113: 504-511.
18. Bonelli, F., and A. Jones. 1992. Continuous fluorescence assay for lecithin:cholesterol acyltransferase using water-soluble phosphatidylcholine. J. Lipid. Res. 33: 1863-1869.
19. Albersheim, P., D. J. Nevins, P. D. English, and A. Karr. 1967. A method for the analysis of sugars in plant cell wall polysaccharides by gas-liquid chromatography. Carbohydr. Res. 5: 340-345.
20. Dell, A., K-H. Khoo, M. Panico, R. A. McDowell, A. T. Etienne, A. J. Reason, and H. R. Morris. 1993. In Glycobiology: a Practical Approach. M. Fukuda, editor. IRL Press, Oxford, 187-222.
21. Dell, A., A. J. Reason, K-H. Khoo, M. Panico, R. A. McDowell, and H. R. Morris. 1993. Mass spectrometry of carbohydrate containing bipolymers. Methods Enzymol. 230: 108-132.
22. Dell, A. 1987. F.A.B. - mass spectrometry of carbohydrates. Adv. Carbohydr. Chem. Biochem. 45: 19-72.
23. Warner, T. G., J. Chang, J. Ferrari, R. Harris, T. McNerney, G. Bennett, J. Burnier, and M. B. Sliwkowski. 1993. Isolation and properties of sialidase from the culture fluid of Chinese hamster ovary cells. Glycobiology. 3: 455-464.
24. Stevenson, S. C., S. Wang, L. Deng, and A. R. Tall. 1993. Human plasma cholesterol transfer protein consists of a mixture of two forms reflecting variable glycosylation at asparagine 341. Biochemistry. 32: 5121-5126.
25. Ben-Zeev, O., M. H. Doolittle, R. C. Davis, J. Elovson, and M. C. Schotz. 1992. Maturation of lipoprotein lipase. Expression of full activity requires glucose trimming but not translocation of the cis-Golgi compartment. J. Biol. Chem. 267: 6219-6227.
26. Albers, J. J., C. H. Chen, and A. G. Lacko. 1986. Isolation, characterization and assay of lecithin-cholesterol acyltransferase. Methods Enzymol. 12: 763-782.
27. Spik, G., B. Bayard, B. Fournet, G. Strecker, S. Bouquelet, and J. Montreuil. 1975. Studies of glycoconjugates. LXIV. Complete structure of two carbohydrate units of human serotransferrin. FEBS Lett. 50: 296-299.
28. Hironaka, T., K. Funikawa, P. C. Esmon, M. A. Fournel, S. Sawada, M. Kato, T. Minaga, and A. Kobata. 1993. Comparative study of the sugar chains of factor VIII, purified from human plasma and from the culture media of baby hamster kidney cells. J. Biol. Chem. 267: 8012-8020.
29. Kagawa, Y., S. Takasaki, J. Utsumi, K. Hosoi, H. Shimizu, N. Kochibe, and A. Kobata. 1988. Comparative study of aspargine-linked sugar chains of natural human interferon-beta 1 produced by three different mammalian cells. J. Biol. Chem. 263: 17508-17515.
30. Sasaki, H., B. Bothner, A. Dell, and M. Fukuda. 1987. Carbohydrate structure of erythropoietin expressed in a Chinese hamster ovary cell line by a human erythropoietin cDNA. J. Biol. Chem. 262: 12059-12076.
31. Spellman, M. W., L. J. Basa, C. K. Leonard, J. A. Chakel, J. V. O'Connor, S. Wilson, and J. van Halbeek. 1989. Carbohydrate structures of human tissue plasminogen activator expressed in Chinese hamster ovary cells. J. Biol. Chem. 264: 14100-14111.
32. Parekh, R. B., R. A. Dwek, J. R. Thomas, G. Opdenakker, T. W. Rademacher, A. J. Wittwer, S. C. Howard, R. Nelson, N. R. Siegel, M. G. Jennings, N. K. Harakas, and J. Feder. 1989. Cell-type specific N-glycosylation of type I and type II human tissue plasminogen activator. Biochemistry. 28: 7644-7662.
33. Fukuda, M. 1985. Cell surface glyconjugates as onco-differentiation markers in hematopoietic cells. Biochim. Biophys. Acta. 780: 119-150.
34. Doi, Y., and T. Nishida. 1983. Microheterogeneity and physical properties of human lecithin-cholesterol acyltransferase. J. Biol. Chem. 258: 5840-5846.
35. O, K., J. S. Hill, X. Wang, and P. H. Pritchard. 1993. Recombinant lecithin:cholesterol acyltransferase containing the Thr 123→Ile mutation esterifies cholesterol in low density lipoproteins but not in high density lipoproteins. J. Lipid. Res. 34: 81-88.