Probing the active site of adenosine deaminase by a pH responsive fluorescent competitive inhibitor

Biophysical Chemistry

Volumn 70, Issue 1, 1998, Pages 41-56

  Caiolfa, Valeria R ^a,b   Gill, David ^a   Parola, Abraham H ^a  

^a BEN GURION UNIVERSITY OF THE NEGEV   (Israel)

^b PFIZER INC   (United States)

Author keywords

Adenosine deaminase; Etheno analogues; Etheno EHNA; Fluorescent competitive inhibitor; pH sensitive inhibitor of ADase; Protonation equilibrium

Indexed keywords

9 (2 HYDROXY 3 NONYL)ADENINE; ADENOSINE DEAMINASE; PENTOSTATIN;

ARTICLE; CHEMICAL MODIFICATION; COMPETITIVE INHIBITION; CONFORMATIONAL TRANSITION; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME KINETICS; FLUORESCENCE POLARIZATION; PRIORITY JOURNAL; PROTON TRANSPORT; SPECTROSCOPY;

ADENINE; ADENOSINE; ADENOSINE DEAMINASE; ANIMALS; BINDING SITES; BINDING, COMPETITIVE; CATALYSIS; CATTLE; ENZYME INHIBITORS; FLUORESCENCE POLARIZATION; FLUORESCENT DYES; HYDROGEN-ION CONCENTRATION; INTESTINES; KINETICS; MOLECULAR STRUCTURE; PENTOSTATIN; PROTEIN BINDING; SPECTROMETRY, FLUORESCENCE; SPECTROPHOTOMETRY;

EID: 0031962979     PISSN: 03014622     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0301-4622(97)00106-3     Document Type: Article

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