A rapid assay for tyrosine hydroxylase activity, an indicator of chronic stress in laboratory and domestic animals

Biological Chemistry

Volumn 379, Issue 1, 1998, Pages 59-63

  Chobotská, Katerina ^a   Arnold, Myrtha ^a   Werner, Patricia ^a   Pliška, Vladimir ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

Adrenal; Catecholamines; Stress; Tetrahydrobiopterine; Thin layer chromatography; Tyrosine hydroxylase, porcine, rat

Indexed keywords

ACETIC ACID; BUTANOL; CATECHOLAMINE DERIVATIVE; DOPA; SILICA GEL; SOLVENT; TYROSINE; TYROSINE 3 MONOOXYGENASE; WATER;

ADRENAL GLAND; ANIMAL TISSUE; ARTICLE; CATECHOLAMINE SYNTHESIS; CHEMICAL REACTION KINETICS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME KINETICS; ENZYME SUBSTRATE; HYDROXYLATION; MICHAELIS CONSTANT; NONHUMAN; PRIORITY JOURNAL; RAT; REPRODUCIBILITY; STRESS; SWINE; THIN LAYER CHROMATOGRAPHY; TISSUE HOMOGENATE;

ANIMALIA; SUIDAE; SUS SCROFA; TRITIUM;

EID: 0031962187     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: 10.1515/bchm.1998.379.1.59     Document Type: Article

References (15)

1. Altman, P.L., and Dittmer, D.S. (eds.) (1961). Blood and Other Body Fluids. (Washington, D.C: Biological Handbooks, Federation of American Societies for Experimental Biology), pp. 11, 75-77.
2. Bartholini, G., and Pletscher, A. (1969). Effect of various decarboxylase inhibitors on the cerebral metabolism of dihydroxyphenylalanin. J. Pharm. Pharmac. 21, 323-324.
3. Fitzpatrick, P.F., Chlumsky, L.J., and Daubner, S.C. (1990). Expression of rat tyrosine hydroxylase in insect tissue culture cells and purification and characterization of the cloned enzyme. J. Biol. Chem. 265, 2042-2047.
4. Levitt, M., Gibb, J.W., Daly, J.W., Lipton, M., and Udenfriend, S. (1967). A new class of tyrosine hydroxylase inhibitors and a simple assay of inhibition in vivo. Biochem. Pharmacol. 16, 1313-1321.
5. Lucock, M.D., Green, M., and Levene, M.I. (1995). Methylfolate modulates potassium evoked neurosecretion: Evidence for a role at the pteridine cofactor level of tyrosine 3-hydroxylase. Neurochem. Res. 20, 727-736.
6. Masserano, J.M., and Weiner, N. (1979). The rapid activation of adrenal tyrosine hydroxylase by decapitation and its relationship to a cyclic AMP-dependent phosphorylating mechanism. Mol. Pharmacol. 16, 513-528.
7. Nagatsu, T., Levitt, M., and Udenfriend, S. (1964a). Tyrosine hydroxylase: The initial step in norepinephrine biosynthesis. J. Biol. Chem. 239, 2910-2917.
8. Nagatsu, T., Levitt, M., and Udenfriend, S. (1964b). A rapid and simple radioassay for tyrosine hydroxylase activity. Anal. Biochem. 9, 122-126.
9. Pliška, V., Hari, J., Heiniger, J., Neuenschwander, S., and Stranzinger, G. (1992). Stress-like changes in the histological structure of pig adrenals and pituitaries: Effect of total body fat but not of predisposition to malignant hyperthermia. J. Anim. Breed. Genet. 109, 51-63.
10. 3H)-tyrosine by charcoal. Life Sci. 39, 2185-2189.
11. Stone, E.A., and McCarty, R. (1983). Adaptation to stress: Tyrosine hydroxylase activity and catecholamine release. Neurosci. Biobehav. Rev. 7, 29-34.
12. Szuran, T., Zimmerman, E., Pliška, V., Pfister, H.P., and Welzl, H. (1991). Prenatal stress effects on exploratory activity and stress-induced analgesia in rats. Dev. Psychobiol. 24, 361-372.
13. von Holst, D. (1986). Vegetative and somatic components of tree shrews' behavior. J. Auton. Nerv. Syst. Suppl. 1986, 657-670.
14. Witte, P.U., and Matthaei, H. (1980). Tyrosinhydroxylase. In: Mikrochemische Methoden für neurobiologische Untersuchungen. (Berlin, Heidelberg: Springer), pp. 37-40.
15. Yamauchi, T. and Fujisawa, H. (1978). A simple and sensitive fluorometric assay for tyrosine hydroxylase. Anal. Biochem. 89, 143-150.