Enrichment of human bone marrow mononuclear phagocytes and characterization of macrophage subpopulations by immunoenzymatic double staining

Histochemical Journal

Volumn 30, Issue 4, 1998, Pages 285-291

  Baldus, Stephan E ^a   Wickenhauser, Claudia ^a   Stefanovic, Alexandra ^a   Schmitz, Beate ^a   Thiele, Juergen ^a   Fischer, Robert ^a  

^a UNIVERSITY OF COLOGNE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; AVIDIN; BIOTIN; CHLORDANE; ENZYME; GALACTOSIDE; HLA DR ANTIGEN; MONOCLONAL ANTIBODY; PEROXIDASE; PHOSPHOGLUCOMUTASE;

ANTIGEN EXPRESSION; ARTICLE; BONE MARROW CELL; CELL SORTER; COMPARATIVE STUDY; COMPLEX FORMATION; CONTROLLED STUDY; CYTOPLASM; HUMAN; HUMAN CELL; IMMUNOASSAY; ISOLATION PROCEDURE; LECTIN BINDING SITE; MACROPHAGE; MAGNETIC AND ELECTROMAGNETIC EQUIPMENT; METHODOLOGY; MONOCYTE; MONONUCLEAR PHAGOCYTE; MORPHOMETRICS; PRIORITY JOURNAL; STAINING; STIMULATION;

ANTIGENS, CD; ANTIGENS, DIFFERENTIATION, MYELOMONOCYTIC; BIOLOGICAL MARKERS; BONE MARROW CELLS; CELL SEPARATION; HUMANS; IMMUNOENZYME TECHNIQUES; LEUKOCYTES, MONONUCLEAR; MACROPHAGES; PHAGOCYTES; STAINING AND LABELING;

EID: 0031960590     PISSN: 00182214     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1003268008228     Document Type: Article

References (35)

1. BALDUS, S.E., THIELE, J., PARK, Y.O., CHARLES, A., MROSS, C., HANISCH, F.G., ZIRBES, T.K., WICKENHAUSER, C. & FISCHER, R. (1995) Carbohydrate and peptide antigens in macrophage populations derived from human bone marrow and milk: an immunomorphological and immunochemical analysis. Histochem. J. 27, 630-8.
2. BHATIA, R., MCGLAVE, P.M., DEWALD, G.W., BLAZAR, B.R. & VERFAILLIE, C.M. (1995) Abnormal function of the bone marrow microenvironment in chronic myelogenous leukemia: role of malignant stromal macrophages. Blood 85, 3636-45.
3. DILLY, S.A., JAGGER, C.J. & SLOANE, J.P. (1993) Stromal cell populations in necropsy bone marrow sections from allogeneic marrow recipients and non-transplant patients. J. Clin. Pathol. 46, 611-16.
4. EICHMÜLLER, S., STEVENSON, P.A. & PAUS, R. (1996) A new method for double immunolabelling with primary antibodies from identical species. J. Immunol. Methods 190, 255-65.
5. FALINI, B., ABDULAZIZ, Z., GERDES, J., CANINO, S., CIANI, C., CORDELL, J.L., KNIGHT, P.M., STEIN, H., GRIGNANI, F., MARTELLI, M.F. & MASON, D.Y. (1986) Description of a sequential staining procedure for double immunoenzymatic staining of pairs of antigens using monoclonal antibodies. J. Immunol. Methods 93, 265-73.
6. FALINI, B., FLENGHI, L., PILERI, S.A., GAMBACORTA, M., BIGERNA, B., DURKOP, H., EITELBACH, F., THIELE, J., PACINI, R., CAVALIERE, A., MARTELLI, M., CARDARELLI, N., SABATTINI, E., POGGI, S. & STEIN, H. (1993) PG-M1: a new monoclonal antibody directed against a fixative-resistant epitope on the macrophage-restricted form of the CD68 molecule. Am. J. Pathol. 142, 1359-72.
7. r mouse macrophage subpopulation specific antigen defined by monoclonal antibodies. J. Immunol. 128, 1221-8.
8. HOLNESS, C.L. & SIMMONS, D.L. (1993) Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins. Blood 81, 1607-13.
9. IRIMURA, T., NORTH, S.M. & NICOLSON, G.L. (1987) Glycoprotein profiles of macrophages at different stages of activation as revealed by lectin binding after electrophoretic separation. Eur. J. Immunol. 17, 73-8.
10. KELLER, R., KEIST, R. & JOLLER, P. (1995) Macrophage response to microbial pathogens: Modulation of the expression of adhesion, CD14, and MHC class II molecules by viruses, bacteria, protozoa and fungi. Scand. J. Immunol. 42, 337-44.
11. KÖLLER, M., WILLHEIM, M., KRUGLUGER, W., KURZ, M., HÖCKER, P., FÖRSTER, O. & BOLTZ-NITULESCU, G. (1996) Immunophenotyping of human bone marrow-derived macrophages. Scand. J. Immunol. 43, 626-32.
12. LEE, S.H (1991) Phenotypic analysis of human bone marrow macrophages. Blood Cells 17, 45-58.
13. LEE, S.H., CROCKER, P.R., WESTABY, S., KEY, N., MASON, D.Y., GORDON, S., WEATHERHALL, D.J. (1988) Isolation and immunocytochemical characterization of human bone marrow stromal macrophages in hemopoietic clusters. J. Exp. Med. 168, 1193-8.
14. 4 isolectin. Proc. Natl. Acad. Sci. USA 79, 166-70.
15. PETRYNIAK, J., HUARD, T.K., NORDBLOM, G.D. & GOLDSTEIN, L.J. (1986) Lectin binding studies on murine peritoneal cells: physicochemical characterization of the binding of lectins from Datura stramonium, Evonymus europaea, and Griffonia simplicifolia to murine peritoneal cells. Arch. Biochem. Biophys. 244, 57-66.
16. PLENAT, F., MARTINET, Y., MARTINET, N. & VIGNAUD, J.M. (1994) Immunohistochemical methods for studying mononuclear phagocytes in tissue sections. J. Immunol. Methods 174, 133-54.
17. PRYZWANSKY, K.B. (1982) Applications of double-label immunofluorescence. In Techniques in Immunohistochemistry, Vol. 1 (edited by BULLOCK, G.R. & PETRUSZ, P.), pp. 77-89. London: Academic Press.
18. SAITO, N., PULFORD, K.A.F., BRETON-GORIUS, J., MASON, D.Y. & CRAMER, E.M. (1991) Ultrastructural localisation of the CD68 macrophage-associated antigen in human blood neutrophils and monocytes. Am. J. Pathol. 139, 1053-9.
19. SATO, S. & HUGHES, R.C. (1994a) Regulation of secretion and surface expression of Mac-2, a galactoside-binding protein of macrophages. J. Biol. Chem. 269, 4424-30.
20. SATO, S. & HUGHES, R.C. (1994b) Control of Mac-2 surface expression on murine macrophage cell lines. Eur. J. Immunol. 24, 216-21.
21. SCHMITZ, B., RADBRUCH, A., KÜMMEL, T., WICKENHAUSER, C., KORB, H., HANSMANN, M.L., THIELE, J., FISCHER, R. (1994) Magnetic activated cell sorting (MACS) - a new immunomagnetic method for megakaryocytic cell isolation: comparison of different separation techniques. Eur. J. Haematol. 52, 267-75.
22. + human T cells following four-step cell separation method including magnetic cell sorting (MACS). J. Immunol. Methods 189, 107-15.
23. STROBL, H., SCHEINECKER, C., CSMARITS, B., MAJDIC, O. & KNAPP, W. (1995) Flow cytometric analysis of intracellular CD68 molecule expression in normal and malignant haemopoiesis. Br. J. Haematol. 90, 774-82.
24. 4-activity. J. Leukoc. Biol. 45, 452-7.
25. THIELE, J., BRAECKEL, C., WAGNER, S., FALINI, B., DIENEMANN, D., STEIN, H. & FISCHER, R. (1992a) Macrophages in normal bone marrow and in chronic myeloproliferative disorders: an immunohistochemical and morphometric study by a new monoclonal antibody (PG-M1) on trephine biopsies. Virchows Arch. A Pathol. Anal. Histol. 421, 33-9.
26. THIELE, J., ROMATOWSKI, C., WAGNER, S., DIENEMANN, D., STEIN, H. & FISCHER, R. (1992b) Macrophages (phagocytic-histiocytic reticular cells) in reactive-inflammatory lesions of the bone marrow and in myelodysplastic syndromes (MDS). An immunohistochemical and morphometric study by use of a new monoclonal antibody (PG-M1). Pathol. Res. Pract. 188, 995-1001.
27. THIELE, J., MEUTER, R.B., TITIUS, R.B., ZANKOVICH, R. & FISCHER, R. (1993) Proliferating cell nuclear antigen expression by erythroid precursors in normal bone marrow, in reactive lesions and in polycythaemia rubra vera. Histopathology 22, 429-35.
28. + human hemopoietic progenitor cells from the peripheral blood: enzyme-, carbohydrate-and immunocytochemistry, morphometry, and ultrastructure. Leukoc. Lymph. 16, 483-91.
29. TITIUS, B.R., THIELE, J., SCHAEFER, H., KREIPE, H. & FISCHER, R. (1994) KI-S1 and proliferating cell nuclear antigen expression of bone marrow macrophages. Acta Haematol. 91, 144-9.
30. VAN DER LOOS, C.M., VAN DEN OORD, J.J., DAS, P.K. & HOUTHOFF, H.J. (1988) Use of commercially available monoclonal antibodies for immunoenzyme double staining. Histochem. J. 20, 409-13.
31. VAN DER LOOS, C.M., BECKER, A.E. & VAN DEN OORD, J.J. (1993) Practical suggestions for successful immunoenzyme double-staining experiments. Histochem. J. 25, 1-13.
32. WARFEL, A.H. & ZUCKER-FRANKLIN, D. (1992) Specific ligation of surface α-D-galactosyl epitopes markedly affects the quantity of four major proteins secreted by macrophages. J. Leukoc. Biol. 52, 80-4.
33. 4: effect on cytotoxicity and protein secretion. J. Cell. Physiol. 147, 265-73.
34. + human hemopoietic progenitor cells of the bone marrow differ from those of the peripheral blood: an immunocytochemical and morphometric study. Acta Haematol. 93, 83-90.
35. WÜRDEN, S. & HOMBERG, U. (1993) A simple method for immunofluorescent double staining with primary antisera from the same species. J. Histochem. Cytochem. 41, 627-30.