3'RACE: Skewed ratio of specific to general PCR primers improves yield and specificity

BioTechniques

Volumn 24, Issue 4, 1998, Pages 575-577

  Bespalova, Irina N ^a,b   Adkins, Steve ^b   Burmeister, Margit ^b  

^a UNIVERSITY OF MICHIGAN   (United States)

^b UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; MESSENGER RNA; POLYADENYLATED RNA;

ARTICLE; BRAIN; GENE AMPLIFICATION; HUMAN; HUMAN TISSUE; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION;

EID: 0031959926     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98244bm12     Document Type: Article

References (5)

1. Frohman, M.A. 1994. On beyond RACE. PCR Methods Appl. 4:S40-S58.
2. Frohman, M.A. 1993. Rapid amplification of complementary DNA ends for generation of full-length complementary DNA's: thermal RACE. Methods Enzymol. 218:340-356.
3. Frohman, M.A., M.K. Dush and G.R. Martin. 1988. Rapid production of full-length cDNA's from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 55:8998-9002.
4. Ponce, M.R. and J.L. Micol. 1992. PCR amplification of long DNA fragments. Nucleic Acids Res. 20:623.
5. Stephen, E.L., M.J. Daly and E.S. Lander. 1991. PRIMER: computer program for automatically selecting PCR primers. MIT Center for Genome Research and Whitehead Institute for Biomedical Research. Cambridge, MA.