Analysis of matrix protein components of the dermis-like structure formed in a long-term culture of human fibroblasts: Type VI collagen is a major component

Journal of Biochemistry

Volumn 123, Issue 4, 1998, Pages 587-595

  Hazeki, Noriko ^a   Yamato, Masayuki ^a   Imamura, Yasutada ^a   Sasaki, Tasuku ^a   Nakazato, Koichi ^a   Yamamoto, Keiichi ^b   Konomi, Hiroshi ^c   Hayashi, Toshihiko ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b CHIBA UNIVERSITY   (Japan)

^c Tokyo Children's Rehab Hospital   (Japan)

Author keywords

Fibroblast; L ascorbic acid 2 phosphate; Type I collagen; Type VI collagen; aminopropionitrile

Indexed keywords

AMINO ACID; ASCORBIC ACID; COLLAGEN TYPE 1; COLLAGEN TYPE 6; CYANOGEN BROMIDE; DODECYL SULFATE SODIUM; MATRIX PROTEIN; PEPSIN A; UREA;

ADULT; AMINO ACID COMPOSITION; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; DERMIS; FIBROBLAST CULTURE; HUMAN; HUMAN CELL; NORMAL HUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS;

EID: 0031959348     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021977     Document Type: Article

References (38)

1. Grinnell, F., Fukamizu, H., Pawelek, P., and Nakagawa, S. (1989) Collagen processing, crosslinking, and fibril bundle assembly in matrix produced by fibroblasts in long-term cultures supplemented with ascorbic acid. Exp. Cell Res. 181, 483-491
2. Hata, R. and Senoo, H. (1989) L-Ascorbic acid 2-phosphate stimulates collagen accumulation, cell proliferation, and formation of a three-dimensional tissuelike substance by skin fibroblasts. J. Cell. Physiol. 138, 8-16
3. Ishikura, N., Yasuda, Y., Iwawaki, R., and Tsukada, S. (1993) Electron microscopic studies of long-term culture of dermal fibroblasts in the presence of L-ascorbic acid phosphate. J. Jpn. P. R.S. 13, 405-412
4. Iwawaki, R., Ishikura, N., and Yasuda, Y. (1990) Electron microscopic studies of dermal fibroblasts cultured in the presence of L-ascorbic acid phosphate. J. Jpn. P.R.S. 10, 467-477
5. Gentzkow, G.D., Iwasaki, S.D., Hershon, K.S., Mengel, M., Prendergast, J.J., Ricotta, J.J., Steed, D.P., and Lipkin, S. (1996) Use of dermagraft, a cultured human dermis, to treat diabetic foot ulcers. Diabetes Care 19, 350-354
6. Chan, D., Lamande, S.R., Cole, W.G., and Bateman, J.F. (1990) Regulation of procollagen synthesis and processing during ascorbate-induced extracellular matrix accumulation in vitro. Biochem. J. 269, 175-181
7. Bateman, J.F. and Golub, S.B. (1994) Deposition and selective degradation of structurally-abnormal type I collagen in a collagen matrix produced by osteogenesis imperfecta fibroblasts in vitro. Matrix Biol. 14, 251-262
8. Valli, M., Zolezzi, F., Mottes, M., Antoniazzi, F., Stanzial, F., Tenni, R., Pignatti, P., and Cetta, G. (1993) Gly85 to Val substitution in proα1(I) chain causes mild osteogenesis imperfecta and introduces a susceptibility to protease digestion. Eur. J. Biochem. 217, 77-82
9. Kuivaniemi, H., Tromp, G., and Prockop, D.J. (1991) Mutations in collagen genes: causes of rare and some common diseases in humans. FASEB J. 5, 2052-2060
10. Layman, D.L., McGoodwin, E.B., and Martin, G.R. (1971) The nature of the collagen synthesized by cultured human fibroblasts. Proc. Natl. Acad. Sci. USA 68, 454-458
11. 3 human skin collagen: Release by pepsin digestion and preponderance in fetal life. J. Biol. Chem. 249, 3225-3231
12. Takeda, Y., Sasaki, T., and Hayashi, T. (1993) Quantitative analysis of type IV collagen by an enzyme immunoassay in the cell layer deposited by cultured fibroblasts: Evidence for distinctly different deposition in two different strains. Connective Tissue 25, 183-188
13. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685
14. Sykes, B., Puddle, B., Francis, M., and Smith, R. (1976) The estimation of two collagens from human dermis by interrupted gel electrophoresis. Biochem. Biophys. Res. Commun. 72, 1472-1480
15. Muraoka, M. and Hayashi, T. (1993) Three polypeptides with distinct biochemical properties are major α chain-size components of type IV collagen in bovine lens capsule. J. Biochem. 114, 358-362
16. Barsh, G.S., Peterson, K.E., and Byers, P.H. (1981) Peptide mapping of collagen chains using CNBr cleavage of proteins within polyacrylamide gels. Collagen Rel. Res. 1, 543-548
17. Matsudaira, P. (1987) Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J. Biol. Chem. 262, 10035-10038
18. Takio, K., Sasagawa, T., and Sakamoto, Y. (1990) Analyses of amino acid composition (in Japanese) in Shin-Seikagaku Jikkenkoza Protein II (Chitani, K., Sakiyama, F., Ota, T., and Suzuki, K., eds.) Vol. 1, pp. 25-53, Tokyo Kagaku-Dojin, Tokyo
19. McCormick, P.J., Chandrasekhar, S., and Millis, A.J.T. (1979) Direct visualization of collagens and procollagens in polyacrylamide gels. Anal. Biochem. 97, 359-366
20. Micko, S. and Schlaepfer, W.W. (1978) Metachromasy of peripheral nerve collagen on polyacrylamide gels stained with Coomassie brilliant blue R-250. Anal. Biochem. 88, 566-572
21. Hattori, S., Sakai, K., Watanabe, K., and Fujii, T. (1996) The induction of metachromasia and circular dichroism of Coomassie brilliant blue R-250 with collagen and histone H1 is due to the low content of hydrophobic amino acid residues in these proteins. J. Biochem. 119, 400-406
22. Chu, M.-L., Pan, T.-C., Conway, D., Kuo, H.-J., Glanville, R.W., Timpl, R., Mann, K., and Deutzmann, R. (1989) Sequence analysis of α1(VI) and α2(VI) chains of human type VI collagen reveals internal triplication of globular domains similar to the A domains of von Willebrand factor and two α2(VI) chain variants that differ in the carboxy terminus. EMBO J. 8, 1939-1946
23. Chu, M.-L., Conway, D., Pan, T.-C., Baldwin, C., Mann, K., Deutzmann, R., and Timpl, R. (1988) Amino acid sequence of the triple-helical domain of human collagen type VI. J. Biol. Chem. 263, 18601-18606
24. 3 in human tissues. Science 183, 1200-1201
25. Hynes, R.O. (1990) Fibronectins, pp. 7-9, 176-187, Springer-Verlag, New York
26. Colombatti, A., Ainger, K., and Colizzi, F. (1989) Type VI collagen: High yields of a molecule with multiple forms of α3 chain from avian and human tissues. Matrix 9, 177-185
27. Trüeb, B. and Winterhalter, K.H. (1986) Type VI collagen is composed of a 200 kd subunit and two 140 kd subunits. EMBO J. 5, 2815-2819
28. Hayashi, T. and Nagai, Y. (1979) Separation of the α chains of type I and III collagens by SDS-polyacrylamide gel electrophoresis. J. Biochem. 86, 453-459
29. r-140000 protein from cutis-laxa fibroblasts in culture. Biochem. J. 227, 491-502
30. Colombatti, A. and Bonaldo, P. (1987) Biosynthesis of chick type VI collagen: II. Processing and secretion in fibroblasts and smooth muscle cells. J. Biol. Chem. 262, 14461-14466
31. Ayad, S., Chambers, C.A., Berry, L., Shuttleworth, A.C., and Grant, M.E. (1986) Type VI collagen and glycoprotein MFPI are distinct components of the extracellular matrix. Biochem. J. 236, 299-302
32. Engvall, E., Hessle, H., and Klier, G. (1986) Molecular assembly, secretion, and matrix deposition of type VI collagen. J. Cell Biol. 102, 703-710
33. Carter, W.G. (1984) The role of intermolecular disulfide bonding in deposition of GP140 in the extracellular matrix. J. Cell Biol. 99, 105-114
34. Chu, M.-L., Zhang, R.-Z., Pan, T.-C., Stokes, D., Conway, D., Kuo, H.-J., Glanville, R., Mayer, U., Mann, K., Deutzmann, R., and Timpl, R. (1990) Mosaic structure of globular domains in the human type VI collagen α3 chain: similarity to von Willebrand Factor, fibronectin, actin, salivary proteins and aprotinin type protease inhibitors. EMBO J. 9, 385-393
35. Odermatt, E., Risteli, J., Van Delden, V., and Timpl, R. (1983) Structural diversity and domain composition of a unique collagenous fragment (intima collagen) obtained from human placenta. Biochem. J. 211, 295-302
36. Fleischmajer, R., Contard, P., Schwartz, E., MacDonald II, E.D., Jacobs II, L., and Sakai, L.Y. (1991) Elastin-associated microfibrils (10 nm) in a three-dimensional fibroblast culture. J. Invest. Dermatol. 97, 638-643
37. Kielty, C.M. and Shuttleworth, C.A. (1993) Synthesis and assembly of fibrillin by fibroblasts and smooth muscle cells. J. Cell Sci. 106, 167-173
38. Oono, T., Specks, U., Eckes, B., Majewski, S., Hunzelmann, N., Timpl, R., and Krieg, T. (1993) Expression of type VI collagen mRNA during wound healing. J. Invest. Dermatol. 100, 329-334