Effects of acid pepsin pretreatment, bile acids and reductants on the excystation of Clonorchis sinensis (Trematoda: Opisthorchiidae) metacercariae in vitro

Parasitology International

Volumn 47, Issue 1, 1998, Pages 29-39

  Ohyama, Fumio ^a  

^a KAWASAKI MEDICAL SCHOOL   (Japan)

Author keywords

Acid pepsin pretreatment; Bile acid; Clonorchis sinensis; Excystation; Metacercaria; Reductant

Indexed keywords

BILE ACID; DEOXYCHOLIC ACID; PEPSIN A; REDUCING AGENT; TAUROCHOLIC ACID;

ARTICLE; CLONORCHIS SINENSIS; CULTURE MEDIUM; HYDROLYSIS; NONHUMAN; PARASITE DEVELOPMENT; PRIORITY JOURNAL;

CLONORCHIS SINENSIS; OPISTHORCHIIDAE; TREMATODA;

EID: 0031958917     PISSN: 13835769     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1383-5769(97)00050-0     Document Type: Article

References (25)

1. Hsü, H. F. and Khaw, O. K. (1936) Studies on certain problems of Clonorchis sinensis 1. On the cysts and second intermediate hosts of C. sinensis in the Peiping area. Chin. Med. J. 50, 1609-1620.
2. Hsü, H. F. and Wang, L. S. (1938) Studies on certain problems of Clonorchis sinensis IV. Notes on the resistance of cysts in fish flesh, the migration route and the morphology of the young worm in the final host. Chin. Med. J. Suppl. 2, 385-400.
3. Yokogawa, M. and Kobayashi, M. (1974) Studies on the excystation of the metacercariae of Clonorchis sinensis. Jpn. J. Parasitol. 23, Suppl., 6-7.
4. Dixon, K. E. (1966) The physiology of excystment of the metacercaria of Fasciola hepatica L. Parasitology 56, 431-456.
5. Tielens, A. G., Van der Meer, P. and Van den Bergh, S. G. (1981) Fasciola hepatica: simple, large-scale, in vitro excystment of metacercariae and subsequent isolation of juvenile liver flukes. Exp. Parasitol. 51, 8-12.
6. Fried, B. and Stromberg, B. E. (1985) In vitro excystment of metacercariae of Fascioloides magna (Trematoda). J. Parasitol. 71, 263-264.
7. Sukhdeo, M. V. K. and Mettrick, D. F. (1986) The behavior of juvenile Fasciola hepatica. J. Parasitol. 72, 492-497.
8. Kawano, J., Ishimaru, T., Umeda, S., Shimizu, A. and Kimura, S. (1987) In vitro excystment of Fasciola sp. metacercariae. Jpn. J. Vet. Sci. 49, 917-919.
9. Ohshima, T., Yoshida, Y. and Kihata, M. (1958) Studies on the excystation of the metacercariae of Paragonimus westermani. (1) Especially on the effect of bile salts. Bull. Inst. Pub. Health. 7, 256-269.
10. Ohshima, T. and Kihata, M. (1958) Studies on the excystation of the metacercariae of Paragonimus westermani. (2) Influence of pepsin pretreatment on the effect of bile salts. Bull. Inst. Pub. Health. 7, 270-274.
11. Chung, Y. B., Kong, Y., Joo, I. J., Cho, S. Y., and Kang, S. Y. (1995) Excystment of Paragonimus westermani metacercariae by endogenous cysteine protease. J. Parasitol. 81, 137-142.
12. Schwert, G. W. and Takenaka, Y. (1955) A spectrophotometric determination of trypsin and chymotrypsin. Biochim. Biophys. Acta. 16, 570-575.
13. Faust, E. C. and Khaw, O.K. (1927) Studies on Clonorchis sinensis (Cobbold). Am. J. Hyg. Monographic Series 88, 1-284.
14. Anson, M. L. and Mirsky, A. E. (1934) The equilibria between native and denatured hemoglobin in salicylate solutions and the theoretical consequences of the equilibrium between native and denatured protein. J. Gen. Physiol. 17, 399-408.
15. Desnuelle, P. (1960) Chymotrypsin. In: The enzymes vol. 4 (Boyer, P. D., Lardy, H. and Myrbaeck, K., eds.), pp. 93-118. Academic Press, New York.
16. Fried, B. and Ramundo, G. B. (1987) Excystation and cultivation in vitro and in ovo of Cyathocotyle bushiensis (Trematoda) metacercariae. J. Parasitol. 73, 541-545.
17. Erasmus, D. A. and Bennet, L. J. (1965) A study of some of the factors affecting excystation in vitro of the metacercarial stages of Holostephanus luehei Szidat, 1936 and Cyathocotyle bushiensis Khan, 1962 (Strigeida: Trematoda). J. Helminthol. 39, 185-196.
18. Helenius, A., McCaslin, D. R., Fries, E. and Tanford, C. (1979) Properties of detergents. Methods Enzymol. 56, 734-749.
19. Exner, T. and Koppel, J. L. (1973) The effect of bile salts on the esterase activities of plasmin and some other enzymes. Biochim. Biophys. Acta. 321, 303-318.
20. Lackie, A. M. (1975) The activation of infective stages of endoparasites of vertebrates. Biol. Rev. 50, 285-323.
21. Dunn, T. S., Ownbey, T. C. and Vannarath, T. (1990) In vitro excystment of the metacercariae of Gynaecotyla adunca and Probolocoryphe uca (Microphallidae) from the fiddler crab, Uca pugilator. Can. J. Zool. 68, 2376-2384.
22. Grob, D. (1946) Proteolytic enzymes I. The control of their activity. J. Gen. Physiol. 29, 219-247.
23. Ward, W. H. and Lundgren, H. P. (1954) The formation, composition, and properties of the keratins. Adv. Protein Chem. 9, 243-295.
24. Kirschner, K. and Bacha, W. J. Jr. (1980) Excystment of Himasthla quissetensis (Trematoda: Echinostomatidae) metacercariae in vitro. J. Parasitol. 66, 263-267.
25. Bass, H. S. and LeFlore, W. B. (1984) In vitro excystment of the metacercaria of Acanthoparyphium spinulosum (Trematoda: Echinostomatidae). Proc. Helminthol. Soc. Wash. 51, 149-153.