Physical characterization of porous materials and correlation with the activity of immobilized enzyme in organic medium

Biocatalysis and Biotransformation

Volumn 16, Issue 1, 1998, Pages 67-85

  Barros, Raúl J ^a   Wehtje, Ernst ^a   Garcia, Fernando A P ^b   Adlercreutz, Patrick ^a  

^a LUND UNIVERSITY   (Sweden)

^b UNIVERSITY OF COIMBRA   (Portugal)

Author keywords

Immobilized chymotrypsin; Internal diffusion; Organic medium; Porous supports; Thiele modulus

Indexed keywords

ACETONITRILE; CHYMOTRYPSIN A; DIPEPTIDE; IMMOBILIZED ENZYME;

ARTICLE; CHEMICAL REACTION KINETICS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME SUBSTRATE; MATHEMATICAL MODEL; PARTICLE SIZE; PEPTIDE SYNTHESIS;

EID: 0031958027     PISSN: 10242422     EISSN: None     Source Type: Journal    
DOI: 10.3109/10242429809040111     Document Type: Article

References (16)

1. Adlercreutz, P. (1996). Modes of using enzymes in organic media. In: Enzymatic Reactions in Organic Media (Eds. A.M.P. Koskinen and A.M Klibanov), Chapman & Hall, Glasgow, UK, pp. 9-42.
2. Al-Duri, B., Robinson, E., McNerlan, S. and Bailie, P. (1995). Hydrolysis of edible oils by lipases immobilized on Hydrophobic supports: Effects of internal support structure. J. Am. Oil Chem. Soc. 72: 1351-1359.
3. Bailey, J.E. and Ollis, D.F. (1986). Biochemical Engineering Fundamentals. 2nd edn McGraw-Hill, New York, USA.
4. Barrett, E.P., Joyner, L.G. and Halenda, P.P. (1951). The determination of pore volume and area distributions in porous substances. I. Computations for nitrogen isotherms. J. Am. Chem. Soc. 73: 373-380.
5. Bosley, J.A. and Clayton, J.C. (1994) Blueprint for a lipase support: Use of hydrophobic controlled-pore glasses as model systems Biotechnol. Bioeng. 43: 934-938.
6. Brunauer, S., Emmet, P.H. and Teller, E. (1938). Adsorption of gases in multimolecular layers. J. Amer. Chem. Soc. 60: 309-319.
7. Faber, K., (1991). A rationale to explain the catalytic activity of solid enzymes in organic solvents. J Mol. Catal. 65: L49-L51.
8. Indlekofer, M., Hoeschele, M., Rizzi, M. and Reuss, M. (1992). Kinetics and engineering studies of lipase-catalyzed transesterification in organic solvent. In: Biocatalysis in Non-Conventional Media, Progress in Biotechnology, Vol. 8, (Eds. J. Tramper, M.H. Vermüe, H.H Beeftink and U. von Stockar), Elsevier, Amsterdam, The Netherlands, pp. 645-652.
9. Ison, A.P., Macrae, A.R., Smith, C.G. and Bosley, J. (1994) Mass transfer effects in solvent-free fat interesterification reactions: Influences on catalyst design. Biotechnol. Bioeng. 43: 122-130
10. Kamat, S., Beckman, E.J. and Russell, A J. (1992). Role of diffusion in nonaqueous enzymology. 1. Theory. Enzyme Microb. Technol. 14: 265-271
11. Luck, T., Kiesser, T. and Bauer. W (1988) Engineering parameters for the application of immobilized lipases in a solvent-free system. In: Biotechnology for the Fats and Oils Industry. World conference (Ed. T.H. Applewhite), AOCS, Champaign, IL, USA, pp 343-345.
12. Smith, J.M. (1981). Chemical Engineering Kinetics. 3rd edn. McGraw-Hill, New York, USA.
13. Stryer, L. (1988). Biochemistry. 3rd edn. Freeman and Co., New York, USA.
14. Triantafyllou, A.Ö., Wang, D., Wehtje, E. and Adlercreutz, P. (1997). Polyacrylamides as immobilization supports for use of hydrolytic enzymes in organic media. Biocatal Biotransf. 15(3): 185-203.
15. Wang, D., Li, M. and He, B. (1992) Immobilization of aminoacylase from Aspergillus oryzae on synthetic modified polyacrylamides. Biotechnol. Appl. Biochem 16: 115-124.
16. Wehtje, E., Adlercreutz, P. and Mattiasson, B. (1993). Improved activity retention of enzymes deposited on solid supports. Biotechnol. Bioeng. 41: 171-178.