COVASIAM: An image analysis method that allows detection of confluent microbial colonies and colonies of various sizes for automated counting

Applied and Environmental Microbiology

Volumn 64, Issue 4, 1998, Pages 1400-1404

  Corkidi, G ^a,b   Diaz Uribe, R ^a   Folch Mallol, J L ^b   Nieto Sotelo, J ^b  

^a UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO   (Mexico)

^b INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

COUNTING TECHNIQUE; COVASIAM; IMAGE ANALYSIS; MICROBIAL COLONY;

ARTICLE; ASPERGILLUS NIDULANS; AUTOMATION; AZOTOBACTER VINELANDII; BACTERIAL COUNT; BACTERIUM COLONY; ESCHERICHIA COLI; FUNGUS GROWTH; IMAGE ANALYSIS; IMAGE PROCESSING; NONHUMAN; PSEUDOMONAS AERUGINOSA; RHIZOBIUM; SACCHAROMYCES CEREVISIAE;

ASPERGILLUS; ASPERGILLUS NIDULANS; ASPERGILLUS NODULANS; AZOBACTER; AZOBACTER VINELANDII; AZOTOBACTER VINELANDII; BACTERIA (MICROORGANISMS); EMERICELLA NIDULANS; ESCHERICHIA COLI; FUNGI; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA; RHIZOBIUM; RHIZOBIUM ETLI; SACCHAROMYCES CEREVISIAE;

EID: 0031957894     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.64.4.1400-1404.1998     Document Type: Article

References (21)

1. Brock, T. D., and M. T. Madigan. 1988. Growth and its control, p. 318. Biology of microorganisms, 5th ed. Prentice-Hall, Englewood Cliffs, N.J.
2. Busta, F. F., E. H. Peterson, D. M. Adams, and M. G. Johnson. 1984. Colony count methods, p. 62-83. In M. L. Speck (ed.). Compendium of methods for the microbiological examination of foods. American Public Health Association, Washington, D.C.
3. Campos, M. E., J. Martinez-Salazar, L. Lloret, S. Moreno, C. Núñez, G. Espín, and G. Soberón-Chavez. 1996. Characterization of the gene coding for GDP-mannose dehydrogenase (algD) from Azotobacter vinetandii. J. Bacteriol. 178:1793-1799.
4. Folch-Mallol, J. L., L. M. Martínez-Mejía, and J. Nieto-Sotelo. Unpublished data.
5. García-Armesto, M. R., M. Prieto, M. L. García-Lopez, A. Otero, and B. Moreno. 1993. Modern microbiological methods for foods: colony count and direct count methods. A review. Microbiologia 9(1):1-13.
6. Haralick, R. M., and L. G. Shapiro. 1992. Binary machine vision (thresholding and segmentation), p. 13-23. Computer and robot vision, vol. I. Addison-Wesley Publishing Company, Inc., New York, KY.
7. Hecht, E. 1989. Spherical mirrors, p. 158-163. Optics, 2nd ed. Addison-Wesley, New York, N. Y.
8. Mukherjee, D. P., A. Pal, S. E. Sarma, and D. D. Majumder. 1995. Bacterial colony counting using distance transform. Int. J. Biomed. Comput. 38(2): 131-140.
9. Nishijima, S., S. Namura, K. Mitsuya, and Y. Asada. 1983. An evaluation, using computerized image analysis, of antimicrobial efficacy of an automatic hand washing machine with ultrasonic wave spraying. J. Dermatol. 20(10): 654-656.
10. Noel, K. D., A. Sánchez, L. Fernández, S. Leemans, and M. A. Ceballos. 1984. Rhizobium phaseoli symbiotic mutants with transposon Tn5. J. Bacteriol. 158:148-155.
11. Otsu, N. 1979. A threshold selection method from gray-level histograms. IEEE Trans. Syst. Man Cybern. SMC-9:62-66.
12. Palmeros, B., L. Güereca, A. Alagón, and G. Soberón-Chávez. 1994. Biochemical characterization of the lipolytic activity of Pseudomonas aeruginosa IGB 83. Process Biochem. 29:207-212.
13. Pickett, D. A., and D. F. Welch. 1995. Evaluation of the automated Bact-Alert system for pediatric blood culturing. Am. J. Clin. Pathol. 103(3):320-323.
14. Russ, J. C. 1990. Binary image editing, p. 153-160. Computer assisted microscopy: the measurement and analysis of images. Plenum Press, New York, N.Y.
15. Sánchez, Y., and S. L. Lindquist. 1990. HSP104 required for induced thermotolerance. Science 248:1112-1115.
16. Spadinger, I., and B, Palcic. 1993. Cell survival measurements at low doses using an automated image cytometry device. Int. J. Radiat. Biol. 63(2):183-189.
17. Stringer, M. A., R. A. Dean, T. C. Sewall, and W. E. Timberlake. 1991. Rodletless, a new Aspergillus developmental mutant induced by directed gene inactivation. Genes Dev. 5:1161-1171.
18. Vincent, L., and P. Soille. 1991. Watersheds in digital spaces: an efficient algorithm based on immersion simulations. IEEE Trans. Pattern Machine Intelligence 13(6):583-598.
19. Walsh, L. R. 1994. Established antimicrobial susceptibility testing methods with a new twist - points to consider and a glimpse of the future. Adv. Exp. Med. Biol. 349:97-105.
20. Wilson, I. G. 1995. Use of the IUL Countermat automatic colony counter for spiral plated total viable counts. Appl. Environ. Microbiol. 61:3158-3160.
21. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985, Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119.