Detection of protein-DNA interactions at β-globin gene cluster in intact human cells utilizing hedamycin as DNA-damaging agent

DNA and Cell Biology

Volumn 17, Issue 4, 1998, Pages 325-333

  Cairns, Murray J ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GLOBIN; DNA; DNA FRAGMENT; HEDAMYCIN; NUCLEAR FACTOR; PROTEIN; TRANSCRIPTION FACTOR AP 1;

ARTICLE; CELL STRAIN K 562; CHROMATIN STRUCTURE; CONTROLLED STUDY; DNA DAMAGE; DNA FOOTPRINTING; DNA SEQUENCE; GENE CLUSTER; GENE LOCUS; GENE TARGETING; GLOBIN GENE; HELA CELL; HUMAN; HUMAN CELL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA INTERACTION;

EID: 0031957190     PISSN: 10445498     EISSN: None     Source Type: Journal    
DOI: 10.1089/dna.1998.17.325     Document Type: Article

References (5)

1. BENNETT, G.N. (1982). Formation of alkali labile linkages in DNA by hedamycin and use of hedaraycin as a probe of protein-DNA complexes. Nucleic Acids Res. 10, 4581-4594.
2. BENZ, E.J., JR., MURNANE, M.J., TONKONOW, B.L., BERMAN, B.W., MAZUR, E.M., CAVALLESCO, C., JENKO, T., SNYDER, E.L., FORGET, B.C., and HOFFMAN, R. (1980). Embryonic-fetal erythroid characteristics of a human leukemic cell line. Proc. Natl. Acad. Sci. USA 77, 3509-3513.
3. BRESNICK, E.H., and FELSENFELD, G. (1993). Evidence that the transcription factor USF is a component of the human β-globin locus control region heteromeric protein complex. J. Biol. Chem. 268, 18824-18834.
4. CAIRNS, M.J. and MURRAY, V. (1996). Influence of chromatin structure on bleomycin-DNA interactions at base pair resolution in the human β-globin gene cluster. Biochemistry 35, 8753-8760.
5. CHARNAY, P. and MANIATIS, T. (1983). Transcriptional regulation of globin gene expression in the human erythroid cell line K562. Science 220, 1281-1283.