Immunohistochemical study of cytochrome b5 in human adrenal gland and in adrenocortical adenomas from patients with Cushing's syndrome

Endocrine Journal

Volumn 45, Issue 1, 1998, Pages 89-95

  Yanase, Toshihiko ^a   Sasano, Hironobu ^b   Yubisui, Toshitsugu ^c   Sakai, Yoshiyuki ^a   Takayanagi, Ryoichi ^a   Nawata, Hajime ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b TOHOKU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^c KOCHI UNIVERSITY   (Japan)

Author keywords

Adrenal androgens; Adrenocortical adenomas; Cushing's syndrome; Cytochrome b5; Immunohistochemistry

Indexed keywords

3(OR 17)BETA HYDROXYSTEROID DEHYDROGENASE; ANDROGEN; CYTOCHROME B5; CYTOCHROME P450 ISOENZYME;

ADRENAL CORTEX ADENOMA; ANDROGEN SYNTHESIS; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; CUSHING SYNDROME; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; RESPIRATORY CHAIN;

EID: 0031954621     PISSN: 09188959     EISSN: None     Source Type: Journal    
DOI: 10.1507/endocrj.45.89     Document Type: Article

References (22)

1. Nakajin S, Shimoda M, Haniu M, Shively JE, Hall PF (1984) C21 steroid side chain cleavage enzyme from porcine adrenal microsomes. J Biol Chem 259: 3971-3976.
2. Zuber MX, Simpson ER, Waterman MR (1986) Expression of bovine 17α-hydroxylase cytochrome P450cDNA in non-steroidogenic (cos 1) cells. Science 234: 1258-1260.
3. Kagimoto M, Winter JSD, Kagimoto K, Simpson ER, Waterman MR (1988) Structural characterization of normal, mutant human steroid 17α-hydroxylase genes: Molecular basis of one example of combined 17α-hydroxylase/17,20-lyase deficiency. Mol Endocrinol 2: 564-570.
4. Matterson KJ, Picado-Leonard J, Chung B-C, Mohandas TK, Miller WL (1986) Assignment of the gene for adrenal P450c17 (steroid 17α-hydroxylase/17,20-lyase) to human chromosome 10. J Clin Endocrinol Metab 63: 789-791.
5. MaCarthy JL, Waterman MR (1988) Co-induction of 17α-hydroxylase and C-17,20-lyase activities in primary cultures of bovine adrenocortical cells in response to ACTH treatment. J Sterod Biochem 29: 307-312.
6. Yanase T, Simpson ER, Waterman MR (1991) 17α-Hydroxylase/17,20-lyase deficiency: From clinical investigation to molecular definition. Endocr Rev 12: 91-108.
7. Parker LN, Odell WD (1980) Control of adrenal androgen secretion. Endocr Rev 1: 392-410.
8. Onoda M, Hall PF (1982) Cytochrome b5 stimulates purified testicular microsomal cutochrome P450 (C21 side-chain cleavage). Biochem Biophys Res Commun 108: 454-460.
9. Shinzawa K, Kominami S, Takemori S (1985) Studies on cytochrome P450 (P45017α, lyase) from guinea pig adrenal microsomes. Dual function of a single enzyme and effect of cytochrome b5. Biochim Biophys Acta 833: 151-160.
10. Yanagibashi K, Hall PF. (1986 ) Role of electron transport in the regulation of the lyase activity of C21 side-chain cleavage P-450 from porcine adrenal and testicular microsomes. J Biol Chem 261: 8429-8433.
11. Katagiri M, Kagawa N, Waterman MR (1995) The role of cytochrome b5 in the biosynthesis of androgens by P450c17. Arch Biochem Biophys 317: 343-347.
12. Sakai Y, Yanase T, Takayanagi R, Nakao R, Nishi Y, Haji M, Nawata H (1993) High expression of cytochrome b5 in adrenocortical adenomas from patients with Cushing's syndrome associated with high secretion of adrenal androgens. J Clin Endocrinol Metab 76: 1286-1290.
13. Sakai Y, Yanase T, Hara T, Takayanagi R, Haji M, Nawata H (1994) In-vitro evidence for the regulation of 17,20-lyase activity by cytochrome b5 in adrenocortical adenomas from patients with Cushing's syndrome. Clin Endocrinol (Oxf) 40: 205-209.
14. Sakai Y, Yanase T, Hara T, Takayanagi R, Haji M, Nawata H (1994) Mechanism of abnormal production of adrenal androgens in patients with adrenocortical adenomas and carcinomas. J Clin Endocrinol Metab 78: 36-40.
15. Sasano H, Mason JI, Sasano N, Nagura H (1990) Immunolocalization of 3β-hydroxysteroid dehydrogenase in human adrenal cortex and in its disorders. Endocr Pathol 1: 94-101.
16. Strittmatter P, Spatz L, Corcoran D, Rogers MJ, Setlow B, Redline R (1974) Purification and properties of rat liver microsomal stearyl coenzyme A desaturase. Proc Natl Acad Sci USA 71: 4565-4569.
17. Kuma F, Prough RA, Masters BSS (1976) Studies on methemoglobin reductase. Arch Biochem Biophys 172: 600-607.
18. Bildebrandt A, Estabrook RW (1976) Evidence for the participation of cytochrome b5 in hepatic microsomal mixed-function oxidation reactions. Arch Biochem Biophys 143: 66-79.
19. Tamburini PP, Gibson GG (1983) Thermodynamic studies of the protein-protein interactions between cytochrome P450 and cytochrome b5. J Biol Chem 1258: 13444-13452.
20. Noshiro M, Ullich V, Omura T (1981) Cytochrome b5 as electron donor for oxy-cytochrome P450. Eur J Biochem 116: 521-526.
21. Aoyama T, Nagata K, Yamazoe Y, Kato R, Matsunaga E, Gelboin HV, Gonzalez FJ (1990) Cytochrome b5 potentiation of cytochrome P-450-catalytic activity demonstrated by a vaccinia virus-mediated in situ reconstitution system. Proc Natl Acad Sci USA 87: 5425-5429.
22. Sasano H, Mason JI, Sasano N (1989) Immunohistochemical study of cytochrome P45017α in human adrenocortical disorders. Human Pathol 20: 113-117.