Concentrating dilute protein solutions for gel electrophoresis

BioTechniques

Volumn 24, Issue 5, 1998, Pages 762-766

  Yitzhaki, Shmuel ^a   Sperling, Joseph ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

TRICHLOROACETIC ACID;

ADSORPTION; ARTICLE; GEL ELECTROPHORESIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS;

EID: 0031954175     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98245bm15     Document Type: Article

References (10)

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3. Roth, M.B., A.M. Zahler and J.A. Stolk. 1991. A conserved family of nuclear phosphoproteins localized to sites of polymerase II transcription. J. Cell Biol. 115:587-596.
4. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY.
5. Sargent, M.G. 1987. Fiftyfold amplification of the Lowry protein assay. Anal. Biochem. 163:476-481.
6. Sauvé, D.M., D.T. Ho and M. Roberge. 1995. Concentration of dilute protein for gel electrophoresis. Anal. Biochem. 226:382-383.
7. Sperling, R., A.J. Koster, C. Melamed- Bessudo, A. Rubinstein, M. Angenitzki, Z. Berkovitch-Yellin and J. Sperling. 1997. Three-dimensional image reconstruction of large nuclear RNP (lnRNP) particles by automated electron tomography. J. Mol. Biol. 267:570-583.
8. Yitzhaki, S., E. Miriami, J. Sperling and R. Sperling. 1996. Phosphorylated Ser/Arg-rich proteins: limiting factors in the assembly of 200S large nuclear ribonucleoprotein particles. Proc. Natl. Acad. Sci. USA 93:8830- 8835.
9. Zahler, A.M., W.S. Lane, J.A. Stolk and M.B. Roth. 1992. SR proteins: a conserved family of pre-mRNA splicing factors. Genes Dev. 6:837-847.
10. Ziegler, J., T. Vogt, O. Miersch and D. Strack. 1997. Concentration of dilute protein solutions prior to sodium dodecyl sulfate polyacrylamide gel electrophoresis. Anal. Biochem. 250:257-260.