Identification of Erwinia amylovora by growth morphology on agar containing copper sulfate and by capsule staining with lectin

Plant Disease

Volumn 82, Issue 2, 1998, Pages 158-164

  Bereswill, Stefan ^a   Jock, Susanne ^a   Bellemann, Peter ^a   Geider, Klaus ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

Amylovoran synthesis; Copper resistance; Plant associated bacteria

Indexed keywords

ABRUS; BACTERIA (MICROORGANISMS); COTONEASTER; ERWINIA AMYLOVORA; MALUS X DOMESTICA; PSEUDOMONAS; PSEUDOMONAS SYRINGAE; RUBUS GLAUCUS;

EID: 0031951270     PISSN: 01912917     EISSN: None     Source Type: Journal    
DOI: 10.1094/PDIS.1998.82.2.158     Document Type: Article

References (30)

1. Bellemann, P., Bereswill, S., Berger, S., and Geider, K. 1994. Visualization of capsule formation by Erwinia amylovora and assays to determine amylovoran synthesis. Int. J. Biol. Macromol. 16:290-296.
2. Bellemann, P., and Geider K. 1992. Localization of transposon insertions in pathogenicity mutants of Erwinia amylovora and their biochemical characterization. J. Gen. Microbiol. 138:931-940.
3. Bennet, R., and Billing, E. 1980. Origin of the polysaccharide component of ooze from plants infected with Erwinia amylovora. J. Gen. Microbiol. 116:341-349.
4. Bereswill, S., Bugert, P., Bruchmüller, I., and Geider, K. 1995. Identification of Erwinia amylovora by PCR with chromosomal DNA. Appl. Environ. Microbiol. 61:2636-2642.
5. Bereswill, S., Jock, S., Aldridge, P., Janse, J. D., and Geider K. Natural levan-deficient Erwinia amylovora strains. Phys. Mol. Plant Pathol. In press.
6. Bereswill, S., Pahl, A., Bellemann P., Zeller W., and Geider K. 1992. Sensitive and species-specific detection of Erwinia amylovora by PCR-analysis. Appl. Environ. Microbiol. 58:3522-3526.
7. Brulez, W., and Zeller, W. 1981. Seasonal changes of epiphytic Erwinia amylovora on ornamentals in relation to weather conditions and course of infections. Acta Home. 117:37-43.
8. Bugert, P., and Geider, K. 1995. Molecular analysis of the ams operon required for exopolysaccharide synthesis of Erwinia amylovora. Mol. Microbiol. 15:917-933.
9. Crosse, J. E., and Goodman, R. N. 1973. A selective medium for and a definitive colony characteristic of Erwinia amylovora. Phytopathology 63:1425-1426.
10. Falkenstein, H., Bellemann, P., Walter, S., Zeller, W., and Geider, K. 1988. Identification of Erwinia amylovora, the fire blight pathogen, by colony hybridization with DNA from plasmid pEA29. Appl. Environ. Microbiol. 54:2798-2802.
11. Falkenstein, H., Zeller, W., and Geider, K. 1989. The 29 kb plasmid, common in strains of Erwinia amylovora, modulates development of fireblight symptoms. J. General Microbiol. 135:2643-2650.
12. Gross, M., Geier, G., Rudolph, K., and Geider, K. 1992. Levan and levansucrase synthesized by the fire blight pathogen Erwinia amylovora. Phys. Mol. Plant Pathol. 40:371-381.
13. Guilford, P. J., Taylor, R. K., Clark, R. G., Hale, C. N., and Forster, R. L. S. 1996. PCR-phased techniques for the detection of Erwinia amylovora. Acta Hortic. 411:53-56.
14. Hodson, N., and Sigee, D. C. 1991. Copper toxicity in Erwinia amylovora: An X-Ray microanalytical study. Scanning Microsc. 5:427-438.
15. Hundle, B., Alberti, M.. Nievelstein, V., Beyer, P., Kleinig, H., Armstrong, G. A., Burke, D. H., and Hearst, J. E. 1994. Functional assignment of Erwinia herbicola Eho10 carotenoid genes expressed in Escherichia coli. Mol. & Gen. Genet. 245:406-416.
16. Ishimaru, C., and Klos, E. J. 1984. New medium for detecting Erwinia amylovora and its use in epidemiological studies. Phytopathology 74:1342-1345.
17. Kado, C. I., and Heskett, M. G. 1970. Selective media for isolation of Agrobacterium, Corynebacterium, Erwinia, Pseudomonas, and Xanthomonas. Phytopathology 60: 969-976.
18. King, E. O., Ward, M. K., and Raney, D. E. 1954, Two simple media for the demonstration of pyocyanin and fluorescin. J. Lab. Clin. Med. 44:301-307.
19. Lelliot, R. A. 1967. The diagnosis of fire blight (Erwinia amylovora) and some diseases caused by Pseudomonas syringae. Eur. Mediterr. Plant Protect. Organ. Publ., Ser. A(45-E):27-34.
20. Lin, C. P., Chen, T. A., Wells, J. M., and van der Zwet, T. 1987. Identification and detection of Erwinia amylovora with monoclonal antibodies. Phytopathology 77:376-380.
21. McManus, P. S., and Jones, A. L. 1995. Detection of Erwinia amylovora by nested PCR and PCR-dot-blot and reverse-blot hybridizations. Phytopathology 85:6.18-623.
22. Metzger, M., Bellemann, P., Bugert, P., and Geider, K. 1994. Genetics of galactose metabolism of Erwinia amylovora and its influence oh polysaccharide-synthesis and virulence of the fire blight pathogen. J. Bacteriol. 176:450-459.
23. Miller, T. D., and Schroth, M. N. 1972. Monitoring the epiphytic population of Erwinia amylovora on pear with a selective medium. Phytopathology 62:1175-1182.
24. Nimtz, M., Mort, A., Wray, V., Domke, T., Zhang, Y., Coplin, D. L., and Geider, K. 1996. Structure of stewartan, the capsular exopolysaccharide from the corn pathogen Erwinia stewartii. Carbohydr. Res. 288:189-201.
25. Nimtz, M., Mort, A., Wray, V., Domke, T., Zhang, Y., Qiu, F., Coplin, D. L., and Geider, K. 1996. Structure of amylovoran, the capsular exopolysaccharide from the fire blight pathogen Erwinia amylovora. Carbohydr. Res. 287:59-76.
26. Roberts, P. 1980. Problems encountered during immunofluorescence diagnosis of fire blight. Plant Pathol. 29:93-97.
27. Steinberger, E. M., and Beer, S. V. 1988. Creation and complementation of pathogenicity mutants of Erwinia amylovora. Mol. Plant-Microbe Interact. 1:135-144.
28. To, K. Y., Lai, E. M., Lee, L. Y., Lin, T. P., Hung, C. H., Chen, C. L., Chang, Y. S., Liu, S. T. 1994. Analysis of the gene cluster encoding carotenoid biosynthesis in Erwinia herbicola Eho13. Microbiol. 140:331-339.
29. Ullrich, M., Bereswill, S., Völksch, B., Fritsche, W., and Geider, K. 1993. Molecular characterization of field isolates of Pseudomonas syringae pv. glycinea differing in coronatine production. J. Gen. Microbiol. 139:1927-1937.
30. Van der Zwet, T., and Keil, H. L. 1979. Fire blight: A bacterial disease of rosaceous plants. Agriculture Handbook no. 510, U.S. Government Printing Office, Washington, DC.