A membrane-associated protein, fliX, is required for an early step in Caulobacter flagellar assembly

Journal of Bacteriology

Volumn 180, Issue 8, 1998, Pages 2175-2185

  Mohr, Christian D ^a   MacKichan, Joanna K ^a   Shapiro, Lucy ^a  

^a STANFORD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE PROTEIN; PROTEIN FLIX; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CAULOBACTER CRESCENTUS; FLAGELLUM; GENE EXPRESSION REGULATION; MEMBRANE BINDING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; STRUCTURAL GENE;

CAULOBACTER; CAULOBACTER CRESCENTUS; CAULOBACTER VIBRIOIDES; NEGIBACTERIA;

EID: 0031948459     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.8.2175-2185.1998     Document Type: Article

References (58)

1. Alley, M. R., J. R. Maddock, and L. Shapiro. 1993. Requirement of the carboxyl terminus of a bacterial chemoreceptor for its targeted proteolysis. Science 259:1754-1757.
2. Anderson, D. K., N. Ohta, J. Wu, and A. Newton. 1995. Regulation of the Caulobacter crescentus rpoN gene and function of the purified sigma 54 in flagellar gene transcription. Mol. Gen. Genet. 246:697-706.
3. Bass, S., Q. Gu, and A. Christen. 1996. Multicopy suppressors of Prc mutant Escherichia coli include two HtrA (DegP) protease homologs (HhoAB), DksA, and truncated RlpA. J. Bacteriol. 178:1154-1161.
4. Benson, A. K., G. Ramakrishnan, N. Ohta, J. Feng, A. J. Ninfa, and A. Newton. 1994. The Caulobacter crescentus FlbD protein acts at ftr sequence elements both to activate and to repress transcription of cell cycle-regulated flagellar genes. Proc. Natl. Acad. Sci. USA 91:4989-4993.
5. Brun, Y. V., and L. Shapiro. 1992. A temporally controlled sigma-factor is required for polar morphogenesis and normal cell division in Caulobacter. Genes Dev. 6:2395-2408.
6. Bryan, R., D. Glaser, and L. Shapiro. 1990. A genetic regulatory hierarchy in Caulobacter development. Adv. Genet. 27:1-31.
7. Carter, P., H. Bedouelle, and G. Winter. 1985. Improved oligonucleotide site-directed mutagenesis using M13 vectors. Nucleic Acids Res. 13:4431-4443.
8. Champer, R., A. Dingwall, and L. Shapiro. 1987. Cascade regulation of Caulobacter flagellar and chemotaxis genes. J. Mol. Biol. 194:71-80.
9. Devereux, D., P. Hableri, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
10. Dingwall, A., J. D. Garman, and L. Shapiro. 1992. Organization and ordered expression of Caulobacter genes encoding flagellar basal body rod and ring proteins. J. Mol. Biol. 228:1147-1162.
11. Dingwall, A., J. W. Gober, and L. Shapiro. 1990. Identification of a Caulobacter basal body structural gene and a cis-acting site required for activation of transcription. J. Bacteriol. 172:6066-6076.
12. Dingwall, A., W. Y. Zhuang, K. Quon, and L. Shapiro. 1992. Expression of an early gene in the flagellar regulatory hierarchy is sensitive to an interruption in DNA replication. J. Bacteriol. 174:1760-1768.
13. Domian, I. J., K. C. Quon, and L. Shapiro. 1997. Cell type-specific phosphorylation and proteolysis of a transcriptional regulator controls the G1-to-S transition in a bacterial cell cycle. Cell 90:415-424.
14. Ely, B. 1991. Genetics of Caulobacter crescentus. Methods Enzymol. 204:372-384.
15. Ely, B., and R. H. Croft. 1982. Transposon mutagenesis in Caulobacter crescentus. J. Bacteriol. 149:620-625.
16. Evinger, M., and N. Agabian. 1977. Envelope-associated nucleoid from Caulobacter crescentus stalked and swarmer cells. J. Bacteriol. 132:294-301.
17. Fleischmann, R. D., M. D. Adams, O. White, R. A. Clayton, E. F. Kirkness, A. R. Kerlavage, C. J. Bull, J. Tomb, B. A. Dougherty, J. M. Merrick, K. McKenney, G. Sutton, W. Fitzhugh, C. Fields, J. D. Gocayne, J. Scott, R. Shirley, L. Liu, A. Glodek, J. M. Kelley, et al. 1995. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496-512.
18. Francis, N. R., G. E. Sosinsky, D. Thomas, and D. J. DeRosier. 1994. Isolation, characterization, and structure of bacterial flagellat motors containing the switch complex. J. Mol. Biol. 235:1261-1270.
19. Gober, J. W., C. H. Boyd, M. Jarvis, E. K. Mangan, M. F. Rizzo, and J. A. Wingrove. 1995. Temporal and spatial regulation of fliP, an early flagellar gene of Caulobacter crescentus that is required for motility and normal cell division. J. Bacteriol. 177:3656-3667.
20. Gober, J. W., and L. Shapiro. 1992. A developmentally regulated Caulobacter flagellar promoter is activated by 3' enhancer and IHF binding elements. Mol. Biol. Cell 3:913-926.
21. Hahnenberger, K. M., and L. Shapiro. 1987. Identification of a gene cluster involved in flagellar basal body biogenesis in Caulobacter crescentus. J. Mol. Biol. 194:91-103.
22. Jenal, U., and L. Shapiro. 1996. Cell cycle-controlled proteolysis of a flagellar motor protein that is asymmetrically distributed in the Caulobacter predivisional cell. EMBO J. 15:2393-2406.
23. Jenal, U., J. White, and L. Shapiro. 1994. Caulobacter flagellar function, but not assembly, requires FliL, a non-polarly localized membrane protein present in all cell types. J. Mol. Biol. 243:227-244.
24. Johnson, R. C., and B. Ely. 1979. Analysis of nonmotile mutants of the dimorphic bacterium Caulobacter crescentus. J. Bacteriol. 137:627-634.
25. Kang, P. J., and E. A. Craig. 1990. Identification and characterization of a new Escherichia coli gene that is a dosage-dependent suppressor of a dnaK deletion mutation. J. Bacteriol. 172:2055-2064.
26. Khambaty, F. M., and B. Ely. 1992. Molecular genetics of theflgI region and its role in flagellum biosynthesis in Caulobacter crescentus. J. Bacteriol. 174: 4101-4109.
27. Kokjohn, T. A., and R. Miller. 1987. Characterization of the Pseudomonas aeruginosa recA analog and its protein product: rec-102 is a mutant allele of the P. aeruginosa PAO recA gene. J. Bacteriol. 169:1499-1508.
28. Macnab, R. 1996. Flagella and motility, p. 123-145. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed. ASM Press, Washington, D.C.
29. Marques, M. V., and J. Gober. 1995. Activation of a temporally regulated Caulobacter promoter by upstream and downstream sequence elements. Mol. Microbiol. 16:279-289.
30. Miller, J. H. 1972. Experiments in molecular genetics, p. 352-355. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
31. Mohr, C. Unpublished data.
32. Mohr, C. D., U. Jenal, and L. Shapiro. 1996. Flagellar assembly in Caulobacter crescentus: a basal body P-ring null mutation affects stability of the L-ring protein. J. Bacteriol. 178:675-682.
33. Mullin, D., S. Minnich, L. S. Chen, and A. Newton. 1987. A set of positively regulated flagellar gene promoters in Caulobacter crescentus with sequence homology to the nif gene promoters of Klebsiella pneumonias. J. Mol. Biol. 195:939-943.
34. Newton, A., N. Ohta, G. Ramakrishnan, D. Mullin, and G. Raymon. 1989. Genetic switching in the flagellar gene hierarchy of Caulobacter requires negative as well as positive regulation of transcription. Proc. Natl. Acad. Sci. USA 86:6651-6655.
35. Ohkubo, S., and K. Yamaguchi. 1997. A suppressor of mutations in the region adjacent to iterons of pSC101 ori. J. Bacteriol. 179:2089-2091.
36. Ohta, N., L. S. Chen, D. A. Mullin, and A. Newton. 1991. Timing of flagellar gene expression in the Caulobacter cell cycle is determined by a transcriptional cascade of positive regulatory genes. J. Bacteriol. 173:1514-1522.
37. O'Neill, E. A., R. H. Hynes, and R. A. Bender. 1985. Recombination deficient mutant of Caulobacter crescentus. Mol. Gen. Genet. 198:275-278.
38. Pugsley, A. P. 1993. The complete general secretory pathway in gram-negative bacteria. Microbiol. Rev. 57:50-108.
39. Quon, K. C., G. T. Marczynski. and L. Shapiro. 1996. Cell cycle control by an essential bacterial two-component signal transduction protein. Cell 84: 83-93.
40. Ramakrishnan, G., and A. Newton. 1990. FlbD of Caulobacter crescentus is a homologue of the NtrC (NRI) protein and activates sigma 54-dependent flagellar gene promoters. Proc. Natl. Acad. Sci. USA 87:2369-2373.
41. Ramakrishnan, G., J. L. Zhao, and A. Newton. 1991. The cell cycle-regulated flagellar gene flbF of Caulobacter crescentus is homologous to a virulence locus (lcrD) of Yersinia pestis. J. Bacteriol. 173:7283-7292.
42. Reisenauer, A. Unpublished data.
43. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
44. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
45. Shapiro, L. 1995. The bacterial flagellum: from genetic network to complex architecture. Cell 80:525-527.
46. Simon, R., U. Piefer, and A. Puhler. 1993. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Bio/Technology 1:784-790.
47. Stephens, C., C. Mohr, C. Boyd, J. Maddock, J. Gober, and L. Shapiro. 1997. Identification of the fliI and fliJ components of the Caulobacter flagellar type III protein secretion system. J. Bacteriol. 179:5355-5365.
48. Stephens, C., A. Reisenauer, R. Wright, and L. Shapiro. 1996. A cell cycle-regulated bacterial DNA methyltransferase is essential for viability. Proc. Natl. Acad. Sci. USA 93:1210-1214.
49. Stephens, C. M., and L. Shapiro. 1993. An unusual promoter controls cell-cycle regulation and dependence on DNA replication of the Caulobacter fliLM early flagellar operon. Mol. Microbiol. 9:1169-1179.
50. Wingrove, J. A., and J. Gober. 1996. Identification of an asymmetrically localized sensor histidine kinase responsible for temporally and spatially regulated transcription. Science 274:597-601.
51. Wingrove, J. A., and J. W. Gober. 1994. A sigma 54 transcriptional activator also functions as a pole-specific represser in Caulobacter. Genes Dev. 8:1839-1852.
52. Wingrove, J. A., E. K. Mangan, and J. W. Gober. 1993. Spatial and temporal phosphorylation of a transcriptional activator regulates pole-specific gene expression in Caulobacter. Genes Dev. 7:1979-1992.
53. Wu, J., A. K. Benson, and A. Newton. 1995. Global regulation of a sigma 54-dependent flagellar gene family in Caulobacter crescentus by the transcriptional activator FlbD. J. Bacteriol. 177:3241-3250.
54. Wu, J., and A. Newton. 1997. Regulation of the Caulobacter flagellar gene hierarchy; not just for motility. Mol. Microbiol. 24:233-239.
55. Xu, H., A. Dingwall, and L. Shapiro. 1989. Negative transcriptional regulation in the Caulobacter flagellar hierarchy. Proc. Natl. Acad. Sci. USA 86: 6656-6660.
56. Yamanaka, K., T. Mitani, T. Ogura, H. Niki, and S. Hiraga. 1994. Cloning, sequencing, and characterization of multicopy suppressors of a mukB mutation in Escherichia coli. Mol. Microbiol. 13:301-312.
57. Yu, J., and L. Shapiro. 1992. Early Caulobacter crescentus genes fliL and fliM are required for flagellar gene expression and normal cell division. J. Bacteriol. 174:3327-3338.
58. Zhuang, W. Y., and L. Shapiro. 1995. Caulobacter FliQ and FliR membrane proteins, required for flagellar biogenesis and cell division, belong to a family of virulence factor export proteins. J. Bacteriol. 177:343-356.