Rapid zymogram for lipase

BioTechniques

Volumn 24, Issue 5, 1998, Pages 754-756

  Yadav, Raman P ^a   Saxena, Rajendra K ^a   Gupta, Rani ^a   Sheba Davidson, W ^a  

^a UNIVERSITY OF DELHI   (India)

Author keywords

[No Author keywords available]

Indexed keywords

TRIACYLGLYCEROL LIPASE;

ARTICLE; ELECTROPHORESIS; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME PURIFICATION; POLYACRYLAMIDE GEL ELECTROPHORESIS;

EID: 0031947222     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98245bm13     Document Type: Article

References (11)

1. Alford, J.A. and E.E. Steinle. 1967. A double layered plate method for the detection of mi- crobial lipolysis. J. Appl, Bacteriol. 30:488- 494.
2. Ghosh, P.K., R.K. Saxena, R. Gupta, R.P. Yadav and S. Davidson. 1996. Microbial lipases: production and applications. Sci. Prog. 79:119-157.
3. Gilbert, E.J., A. Cornish and C.W. Jones. 1991. Purification and properties of extracellular lipase from Pseudomonas aeruginosa EF2. J. Gen. Microbiol. 737:2223-2229.
4. Laemmli, U.K. 1970. Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227:680-685.
5. Macrae, A.R. and R.C. Hammond. 1985. Present and future applications of Upases. Biotechnol. Genet. Eng. Rev. 3:193-217.
6. Nahas, E. 1988. Control of lipase production by Rhizopus oligosporus under various growth conditions. J. Gen. Microbiol. 734:227-233.
7. Parmar, V.S., H.N. Pati, R.P. Yadav, A. Kumar, K.S. Bisht, R. Gupta, S. Davidson, Poonam and R.K. Saxena. 1998. Utility of a novel lipase from Aspergillus terreus in deacetylation reactions. Biocatal. Biotransform. 76:17-25.
8. Verger, R., C. Riviere, H. Moreau, Y. Gargouri, E. Rogalska, S. Nury, A. Moulin, F. Ferrato et al. 1991. Enzyme kinetics of lipolysis, p. 105-116. In L. Alberghina, R.D. Schmid and R. Verger (Eds.), Lipases: Structure, Mechanism and Genetic Engineering. VCH Publishers, New York.
9. Yadav, R.P., R.K. Saxena, R. Gupta and S. Davidson. 1997. Production of biosurfactant from sugar alcohols and natural triglycerides by Aspergillus terreus lipase. J. Sci. Indus. Res. 56:479-482.
10. Yadav, R.P., R.K. Saxena, R. Gupta and S. Davidson. Lipase production from Aspergillus and Penicillium spp. Folia Microbiol. (In press)
11. Yadav, R.P., R.K. Saxena, R. Gupta and S. Davidson. Purification and characterization of a regiospecific lipase from Aspergillus terreus. Biotechnol. Appl. Biochem. (In press)