One-step procedure for screening recombinant plasmids by size

BioTechniques

Volumn 24, Issue 5, 1998, Pages 748-750

  Beuken, Erik ^a   Vink, Cornells ^a   Bruggeman, Cathrien A ^a  

^a MAASTRICHT UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; DNA DETERMINATION; DNA SEQUENCE; DNA TEMPLATE; RECOMBINANT PLASMID;

EID: 0031946147     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98245bm10     Document Type: Article

References (8)

1. Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl. 1989. Escherichia coli, plasmids, and bacteriophages, p. 1.6.2-1.6.3. Current Protocols in Molecular Biology. John Wiley & Sons, New York.
2. Gussow, D. and T. Clackson, 1989. Direct clone characterization from plaques and colonies by polymerase chain reaction. Nucleic Acids Res. 77:4000.
3. Liu, Z. and N.C. Mishra. 1995. Single-tube method for plasmid miniprep from large numbers of clones for direct screening by size or restriction digestion. BioTechniques 75:214- 217.
4. Mak, Y.M., R. Sornarajah and K.K. Ho. 1991. A rapid procedure for detecting recombinant plasmids using butanol extraction. BioTechniques 77:723.
5. Ng, W.-L., M. Schummer, F.D. Cirisano, R.L. Baldwin, B.Y. Karlan and L. Hood. 1996. High-throughput plasmid mini preparations facilitated by micro-mixing. Nucleic Acids Res. 24:5045-5047.
6. Tarczynski, M.C., W.J. Meyer, J.J. Min, K.A. Wood and R.J. Hellwig. 1994. Two- minute miniprep method for plasmid DNA isolation. BioTechniques 76:514-519.
7. Wang, K., I. Can and L. Hood. 1995. A microtiter plate-based high-throughput DNA purification method. Anal. Biochem. 226:85-90.
8. Xie, W.G. and M. Potts. 1989. Quick screening of plasmid deletion clones carrying inserts of desired size. Gene Anal. Tech. 6:17-20.