A new method for intense staining of myelin

Journal of Histochemistry and Cytochemistry

Volumn 46, Issue 4, 1998, Pages 541-545

  McNally, Karen J ^a   Peters, Alan ^a  

^a BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

DAB reaction; Horse serum; Myelin; Silver intensification

Indexed keywords

4 DIMETHYLAMINOAZOBENZENE; MYELIN; SILVER;

ANIMAL TISSUE; ARTICLE; BRAIN; CAT; ELECTRON MICROSCOPY; GRAY MATTER; HORSE; IMMERSION; MONKEY; MYELIN SHEATH; MYELINATED NERVE; NONHUMAN; PHOTOGRAPHY; PRIORITY JOURNAL; RAT; SERUM; SPINAL CORD; STAINING; TECHNIQUE;

ANIMALIA; EQUUS CABALLUS; FELIS CATUS;

EID: 0031945607     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215549804600415     Document Type: Article

References (10)

1. Fairén A, Peters A, Saldanha J (1977) A new procedure for examining Golgi impregnated neurons by light and electron microscopy. J Neurocytol 6:311-337
2. Gallyas F, Görcs T, Merchenthaler I (1982) High-grade intensification of the end-product of the diaminobenzidine reaction for peroxidase histochemistry. J Histochem Cytochem 30:183-184
3. Gerrits PO, Brekelmans-Bartels M, Mast L, 's-Gravenmade EJ, Horobin RW, Holstege G (1992) Staining myelin and myelin-like degradation products in the spinal cords of chronic experimental allergic encephalomyelitis (Cr-EAE) rats using Sudan Black B staining of glycol methacrylate-embedded material. J Neurosci Methods 45:99-105
4. Görcs TJ, Léránth C, MacLusky NJ (1986) The use of gold-substituted silver-intensified diaminobenzidine (DAB) and non-intensified DAB for simultaneous electron microscopic immunoperoxidase labeling of tyrosine hydroxylase and glutamic acid decarboxylase immunoreactivity in the rat medial preoptic area. J Histochem Cytochem 34:1439-1447
5. Klüver H, Barrera E (1953) A method for the combined staining of cells and fibers in the nervous system. J Neuropathol Exp Neurol 12:400-403
6. Lillie RD (1965) Histologic Technic and Practical Histochemistry. 3rd ed. New York, McGraw-Hill
7. Peters A, Cifuentes JM, Sethares C (1997) The organization of pyramidal cells in area 18 of the rhesus monkey. Cerebral Cortex 7:405-421
8. Peters A, Sethares C (1996) Myelinated axons and the pyramidal cell modules in monkey primary visual cortex. J Comp Neurol 365:232-255
9. Schmued LC (1990) A rapid, sensitive histochemical stain for myelin in frozen brain sections. J Histochem Cytochem 38:717-720
10. Weigert C (1884) Ausführliche Beschreibung der in no. 2 dieser Zeitschrift erwähnten neuen Färbungsmethode für das Central-nervensystem. Fortschr Deutsch Med 2:190-192