Moloney murine leukemia virus protease expressed in bacteria is enzymatically active

Archives of Virology

Volumn 143, Issue 2, 1998, Pages 381-388

  Cannon, K ^a,b   Qin, L ^a,c   Schumann, G ^a   Boeke, J D ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b Life Technologies   (United States)

^c GENVEC INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI; MOLONEY MURINE LEUKEMIA VIRUS; PROTEIN; VIRAL PROTEASE; VIRUS REPLICATION; VIRUS TRANSCRIPTION;

ENDOPEPTIDASES; ESCHERICHIA COLI; FUSION PROTEINS, GAG-POL; MOLECULAR WEIGHT; MOLONEY MURINE LEUKEMIA VIRUS; RECOMBINANT PROTEINS;

EID: 0031941843     PISSN: 03048608     EISSN: None     Source Type: Journal    
DOI: 10.1007/s007050050294     Document Type: Article

References (20)

1. Andreánsky M, Hrusková-Heidingsfeldová O, Sedlácek J, Konvalinka J, Bláha I, Jecmen P, Horejsí M, Strop P, Fábry M (1991) High-level expression of enzymatically active bovine leukemia virus proteinase in E. coli. FEBS Lett 287: 129-132
2. Crawford S, Goff SP (1985) A deletion mutation in the 5′ part of the pol gene of Moloney Murine leukemia virus blocks proteolytic processing of the gag and pol polyproteins. J Virol 53: 899-907
3. Dickson C, Eisenman R, Fan H, Hunter E, Teich N (1984) Protein biosynthesis and assembly. In: Weiss R, Teich N, Varmus H, Coffin J (eds) RNA tumor viruses. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, pp 513-648
4. Feng Y-X, Copeland TD, Orozlan S, Rein A, Levin JG (1990) Identification of amino acids inserted during suppression of UAA and AGA termination codons at the gag-pol junction of Moloney murine leukemia virus. Proc Natl Acad Sci USA 87: 8860-8863
5. Feng Y-X, Yuan H, Rein A, Levin JG (1992) A bipartite signal for readthrough suppression in murine leukemia virus mRNA: an eight-nucleotide purine-rich sequence immediately downstream of the gag termination codon followed by an RNA pseudoknot. J Virol 66: 5127-5132
6. Flaman J-M, Frebourg T, Moreau V, Charbonnier F, Martin C, Ishioka C, Friend SH, Iggo R (1994) A rapid PCR fidelity assay. Nucleic Acids Res 22: 3259-3260
7. Hayakawa T, Misumi Y, Kobayashi M, Ohi Y, Fujisawa Y, Kakinuma A, Hatanaka M (1991) Expression of human T-cell leukemia virus type I protease in E. coli. Biochem Biophys Res Commun 181: 1281-1287
8. Higuchi R, Krummel B, Saiki R (1988) A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions. Nucleic Acids Res 16: 7351-7367
9. Kotler M, Katz RA, Skalka AM (1988) Activity of avian retroviral protease expressed in E. coli. J Virol 62: 2696-2700
10. Kotler M, Arad G, Hughes SH (1992) Human immunodeficiency virus type 1 gag-protease fusion proteins are enzymatically active. J Virol 66: 6781-6783
11. Le Grice SFJ, Ette R, Mills J, Mous J (1989) Comparison of the human immunodeficiency virus type 1 and 2 proteases by hybrid gene construction and trans-complementation. J Biol Chem 264: 14902-14908
12. Lundberg KS, Shoemaker DD, Adams MWW, Short JM, Sorge JA, Muthur EJ (1991) High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. Gene 108: 1-6
13. Marczinovits I, Boros I, El Jarrah F, Fust G, Molnár J (1993) Expression in E. coli and in vitro processing of HIV-1 p24 fusion protein. J Biotechnol 31: 225-232
14. Menéndez-Arias L, Gotte D, Oroszlan S (1993) Moloney murine leukemia virus protease: bacterial expression and characterization of the purified enzyme. Virology 196: 557-563
15. Menéndez-Arias L, Weber IT, Soss J, Harrison RW, Gotte D, Oroszlan S (1994) Kinetic and modeling studies of subsites S4-S3' of Moloney murine leukemia virus protease. J Biol Chem 269: 16795-16801
16. Natsoulis G, Seshaiah P, Federspiel MJ, Rein A, Hughes SH, Bocke JD (1995) Targeting of a nuclease to murine leukemia virus capsids inhibits viral multiplication. Proc Natl Acad Sci USA 92: 364-368
17. Oroszlan S, Luftig RB (1990) Retroviral proteinases. In: Swanstrom R Vogt PK (eds) Retroviruses-strategies of replication. Springer, New York, pp 153-185
18. Rein A, Levin JG (1992) Readthrough suppression in the mammalian type C retroviruses and what it has taught us. New Biol 4: 283-289
19. Schumann G, Qin L, Rein A, Natsoulis G, Boeke JD (1996) Therapeutic effect of Gag-nuclease fusion protein on retrovirus-infected cell cultures. J Virol 70: 4329-4337
20. Yoshioka K, Honma H, Zushi M, Knodo S, Togashi S, Miyaek T, Shiba T (1990) Virus-like particle formation of Drosophila copia through autocatalytic processing. EMBO J 9: 535-541